EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae

The in vitro probiotic capacity of a native strain of Saccharomyces cerevisiae (A) was evaluated and comparedwith a commercial strain (B) used as a probiotic. The effect of the concentration of sugarcane molasses (10, 20and 30% (w/v)) on the biomass production was investigated and kinetic parameters...

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Bibliographic Details
Main Authors: Ángela Ortiz, Joanna Reuto, Erika Fajardo, Sandra Sarmiento, Andrea Aguirre, Gustavo Arbeláez, David Gómez, Balkys Quevedo-Hidalgo
Format: Article
Language:English
Published: Pontificia Universidad Javeriana 2008-09-01
Series:Universitas Scientiarum
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Online Access:http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1418/880
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Summary:The in vitro probiotic capacity of a native strain of Saccharomyces cerevisiae (A) was evaluated and comparedwith a commercial strain (B) used as a probiotic. The effect of the concentration of sugarcane molasses (10, 20and 30% (w/v)) on the biomass production was investigated and kinetic parameters were determined. The bestmolasses concentration was 20% (w/v) and differences in biomass production on molasses medium betweenstrain A (28 g/L) and control strain B (3 g/L) were observed. In vitro tests such as tolerance to bile salts, pH andgastric juices were carried out, and no differences in growth between strain A and B were found. Cholesterolreduction on presence of bile salts after 12 hours of incubation was of 54% for strain A and 58% for strain B.Both strains showed adherence to Caco-2 cells. Results reveal that strain A possesses in vitro probiotic propertiesthat can be verified with further in vivo studies to confirm its suitability as probiotic in animal nutrition.
ISSN:0122-7483
2027-1352