The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat
Abstract Background Starch, a major component of wheat (Triticum aestivum L.) grain, plays a crucial role in determining processing quality. Granule-bound starch synthase I (GBSSI), the enzyme primarily responsible for elongating α-1,4-glucan chains into linear amylose molecules, is a key determinan...
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2025-05-01
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| Online Access: | https://doi.org/10.1186/s12864-025-11630-w |
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| author | Sujon Kumar Yulong Li Jia Zheng Jing Liu Qiang Xu Yazhou Zhang Huaping Tang Pengfei Qi Mei Deng Jian Ma Guoyue Chen Yuming Wei Youliang Zheng Qiantao Jiang |
| author_facet | Sujon Kumar Yulong Li Jia Zheng Jing Liu Qiang Xu Yazhou Zhang Huaping Tang Pengfei Qi Mei Deng Jian Ma Guoyue Chen Yuming Wei Youliang Zheng Qiantao Jiang |
| author_sort | Sujon Kumar |
| collection | DOAJ |
| description | Abstract Background Starch, a major component of wheat (Triticum aestivum L.) grain, plays a crucial role in determining processing quality. Granule-bound starch synthase I (GBSSI), the enzyme primarily responsible for elongating α-1,4-glucan chains into linear amylose molecules, is a key determinant of starch quality. In this study, a mutant population of the wheat cultivar SM126, a high-quality variety form Sichuan, China, was generated using ethyl methanesulfonate (EMS) mutagenesis. This research investigates the effects of GBSSI inactivation on starch structure and functionality. Results A waxy mutant (Wx-Abd) was identified by screening an M4 seed library with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of grain endosperm flour. DNA sequencing revealed a single nucleotide polymorphism (SNP) in the fourth exon, causing a premature stop codon and inactivation of the Wx-Abd allele. In previous work, the Wx-abD mutant was identified in the M2 generation, and crossing the M2-31 line with the M4-6165 line produced four distinct Wx protein subunits in the SM126 background. Comparisons between the Wx-abd line and the wild-type SM126 (Wx-AbD) showed significant differences in starch properties. The Wx-abd line exhibited reduced Wx gene expression, a distinct surface depression on starch granules, and a higher proportion of B-type starch granules. Notably, it exhibited significantly lower amylose content (7.02%) compared to SM126 (22.32%), along with a reduction in total starch content. Additionally, the Wx-abd line showed a higher gelatinization temperature. Conclusion Inactivation of GBSSI in the Wx-abd line resulted in altered starch structure, particularly a decrease in amylose content and changes in granule morphology. These findings suggest that the Wx-abd line represents a valuable genetic resource for wheat breeding programs focused on improving starch quality for food production, with its high agronomic performance making it suitable for further breeding applications. |
| format | Article |
| id | doaj-art-dc5e67f06fc94252b2c7f1633e2d72c8 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-05-01 |
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| series | BMC Genomics |
| spelling | doaj-art-dc5e67f06fc94252b2c7f1633e2d72c82025-08-20T03:48:15ZengBMCBMC Genomics1471-21642025-05-0126111510.1186/s12864-025-11630-wThe impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheatSujon Kumar0Yulong Li1Jia Zheng2Jing Liu3Qiang Xu4Yazhou Zhang5Huaping Tang6Pengfei Qi7Mei Deng8Jian Ma9Guoyue Chen10Yuming Wei11Youliang Zheng12Qiantao Jiang13State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityKey Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry, Wuliangye Group Co., Ltd.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural UniversityAbstract Background Starch, a major component of wheat (Triticum aestivum L.) grain, plays a crucial role in determining processing quality. Granule-bound starch synthase I (GBSSI), the enzyme primarily responsible for elongating α-1,4-glucan chains into linear amylose molecules, is a key determinant of starch quality. In this study, a mutant population of the wheat cultivar SM126, a high-quality variety form Sichuan, China, was generated using ethyl methanesulfonate (EMS) mutagenesis. This research investigates the effects of GBSSI inactivation on starch structure and functionality. Results A waxy mutant (Wx-Abd) was identified by screening an M4 seed library with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of grain endosperm flour. DNA sequencing revealed a single nucleotide polymorphism (SNP) in the fourth exon, causing a premature stop codon and inactivation of the Wx-Abd allele. In previous work, the Wx-abD mutant was identified in the M2 generation, and crossing the M2-31 line with the M4-6165 line produced four distinct Wx protein subunits in the SM126 background. Comparisons between the Wx-abd line and the wild-type SM126 (Wx-AbD) showed significant differences in starch properties. The Wx-abd line exhibited reduced Wx gene expression, a distinct surface depression on starch granules, and a higher proportion of B-type starch granules. Notably, it exhibited significantly lower amylose content (7.02%) compared to SM126 (22.32%), along with a reduction in total starch content. Additionally, the Wx-abd line showed a higher gelatinization temperature. Conclusion Inactivation of GBSSI in the Wx-abd line resulted in altered starch structure, particularly a decrease in amylose content and changes in granule morphology. These findings suggest that the Wx-abd line represents a valuable genetic resource for wheat breeding programs focused on improving starch quality for food production, with its high agronomic performance making it suitable for further breeding applications.https://doi.org/10.1186/s12864-025-11630-wWaxy wheatGBSSIGene expressionStarch propertiesThermodynamic properties |
| spellingShingle | Sujon Kumar Yulong Li Jia Zheng Jing Liu Qiang Xu Yazhou Zhang Huaping Tang Pengfei Qi Mei Deng Jian Ma Guoyue Chen Yuming Wei Youliang Zheng Qiantao Jiang The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat BMC Genomics Waxy wheat GBSSI Gene expression Starch properties Thermodynamic properties |
| title | The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat |
| title_full | The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat |
| title_fullStr | The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat |
| title_full_unstemmed | The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat |
| title_short | The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat |
| title_sort | impact of gbssi inactivation on starch structure and functionality in ems induced mutant lines of wheat |
| topic | Waxy wheat GBSSI Gene expression Starch properties Thermodynamic properties |
| url | https://doi.org/10.1186/s12864-025-11630-w |
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