Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation
Chronic wound infections are attributed to delayed tissue repair, which remains a major clinical challenge in long-term health care. Particularly, infections with antibiotic resistance have more serious effects on health, often resulting in unsuccessful treatments. Thus, antimicrobial peptide (AMP)-...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | International Journal of Pharmaceutics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259015672400063X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846119441802199040 |
|---|---|
| author | Sirikwan Sangboonruang Natthawat Semakul Kiattikhun Manokruang Nuttawut Khammata Kanyaluck Jantakee Katanchalee Mai-Ngam Satrawut Charoenla Phadungkiat Khamnoi Kanokwan Saengsawang Usanee Wattananandkul Sorasak Intorasoot Khajornsak Tragoolpua |
| author_facet | Sirikwan Sangboonruang Natthawat Semakul Kiattikhun Manokruang Nuttawut Khammata Kanyaluck Jantakee Katanchalee Mai-Ngam Satrawut Charoenla Phadungkiat Khamnoi Kanokwan Saengsawang Usanee Wattananandkul Sorasak Intorasoot Khajornsak Tragoolpua |
| author_sort | Sirikwan Sangboonruang |
| collection | DOAJ |
| description | Chronic wound infections are attributed to delayed tissue repair, which remains a major clinical challenge in long-term health care. Particularly, infections with antibiotic resistance have more serious effects on health, often resulting in unsuccessful treatments. Thus, antimicrobial peptide (AMP)-based therapy holds promise as a potential therapeutic approach to overcoming drug resistance. Conventional wound dressing is a passive strategy for wound care that is not capable of eradicating pathogens and promoting tissue repair. In this study, we aim to construct an advanced wound dressing; a thermo-responsive hydrogel incorporated with lactoferricin (Lfcin) niosome (Lfcin-Nio/hydrogel) for bacterial pathogen treatment. The Lfcin-loaded niosome (Lfcin-Nio) has a particle size of 396.91 ± 20.96 nm, 0.38 ± 0.01 of PdI, −10.5 ± 0.3 mV of ζ potential, and 72.30 ± 7.05 % Lfcin entrapment efficiency. Lfcin-Nio exhibited broad antibacterial activity on both drug-susceptible and drug-resistant strains, and also on bacteria residing in the biofilm matrix. The Lfcin-Nio/hydrogel was fabricated from 0.5 % w/v poloxamer 188–20 % w/v poloxamer 407, and supplemented with Lfcin-Nio and epidermal growth factor (EGF). The physical properties of Lfcin-Nio/hydrogels showed elasticity, swelling ability, and strong injectability with responsiveness to 33–37 °C temperatures. The biological properties of Lfcin-Nio/hydrogels exhibited a bactericidal effect against drug-resistant strains of S. aureus and P. aeruginosa, and showed less toxicity to the human skin fibroblast. It also promoted the healing of scratches by 55 % within 6 h, compared to the wound closure rate of 20 % in the cell control. The inflammatory response of the Lfcin-Nio/hydrogel-treated cells was reduced via suppression of IL-1β and COX-2 mRNA expressions. From this study, Lfcin-Nio/hydrogels can be suggested as a modern wound dressing that possesses multifunctional and beneficial properties for the management of chronic wound infections. |
| format | Article |
| id | doaj-art-dc39a9262eaa4e12a79505605ae50a13 |
| institution | Kabale University |
| issn | 2590-1567 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Pharmaceutics: X |
| spelling | doaj-art-dc39a9262eaa4e12a79505605ae50a132024-12-17T05:00:39ZengElsevierInternational Journal of Pharmaceutics: X2590-15672024-12-018100291Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammationSirikwan Sangboonruang0Natthawat Semakul1Kiattikhun Manokruang2Nuttawut Khammata3Kanyaluck Jantakee4Katanchalee Mai-Ngam5Satrawut Charoenla6Phadungkiat Khamnoi7Kanokwan Saengsawang8Usanee Wattananandkul9Sorasak Intorasoot10Khajornsak Tragoolpua11Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Office of Research Administration, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandNational Metal and Materials Technology Center, National Science and Technology Development Agency, Pathumthani 12120, ThailandNational Metal and Materials Technology Center, National Science and Technology Development Agency, Pathumthani 12120, ThailandDiagnostic Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Medical Technology, Lampang Hospital, Lampang 52000, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Corresponding author.Chronic wound infections are attributed to delayed tissue repair, which remains a major clinical challenge in long-term health care. Particularly, infections with antibiotic resistance have more serious effects on health, often resulting in unsuccessful treatments. Thus, antimicrobial peptide (AMP)-based therapy holds promise as a potential therapeutic approach to overcoming drug resistance. Conventional wound dressing is a passive strategy for wound care that is not capable of eradicating pathogens and promoting tissue repair. In this study, we aim to construct an advanced wound dressing; a thermo-responsive hydrogel incorporated with lactoferricin (Lfcin) niosome (Lfcin-Nio/hydrogel) for bacterial pathogen treatment. The Lfcin-loaded niosome (Lfcin-Nio) has a particle size of 396.91 ± 20.96 nm, 0.38 ± 0.01 of PdI, −10.5 ± 0.3 mV of ζ potential, and 72.30 ± 7.05 % Lfcin entrapment efficiency. Lfcin-Nio exhibited broad antibacterial activity on both drug-susceptible and drug-resistant strains, and also on bacteria residing in the biofilm matrix. The Lfcin-Nio/hydrogel was fabricated from 0.5 % w/v poloxamer 188–20 % w/v poloxamer 407, and supplemented with Lfcin-Nio and epidermal growth factor (EGF). The physical properties of Lfcin-Nio/hydrogels showed elasticity, swelling ability, and strong injectability with responsiveness to 33–37 °C temperatures. The biological properties of Lfcin-Nio/hydrogels exhibited a bactericidal effect against drug-resistant strains of S. aureus and P. aeruginosa, and showed less toxicity to the human skin fibroblast. It also promoted the healing of scratches by 55 % within 6 h, compared to the wound closure rate of 20 % in the cell control. The inflammatory response of the Lfcin-Nio/hydrogel-treated cells was reduced via suppression of IL-1β and COX-2 mRNA expressions. From this study, Lfcin-Nio/hydrogels can be suggested as a modern wound dressing that possesses multifunctional and beneficial properties for the management of chronic wound infections.http://www.sciencedirect.com/science/article/pii/S259015672400063XLfcin-NioThermo-responsive hydrogelAnti-bacterial activityWound healingAnti-inflammation |
| spellingShingle | Sirikwan Sangboonruang Natthawat Semakul Kiattikhun Manokruang Nuttawut Khammata Kanyaluck Jantakee Katanchalee Mai-Ngam Satrawut Charoenla Phadungkiat Khamnoi Kanokwan Saengsawang Usanee Wattananandkul Sorasak Intorasoot Khajornsak Tragoolpua Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation International Journal of Pharmaceutics: X Lfcin-Nio Thermo-responsive hydrogel Anti-bacterial activity Wound healing Anti-inflammation |
| title | Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation |
| title_full | Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation |
| title_fullStr | Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation |
| title_full_unstemmed | Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation |
| title_short | Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation |
| title_sort | multifunctional poloxamer based thermo responsive hydrogel loaded with human lactoferricin niosomes in vitro study on anti bacterial activity accelerate wound healing and anti inflammation |
| topic | Lfcin-Nio Thermo-responsive hydrogel Anti-bacterial activity Wound healing Anti-inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S259015672400063X |
| work_keys_str_mv | AT sirikwansangboonruang multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT natthawatsemakul multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT kiattikhunmanokruang multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT nuttawutkhammata multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT kanyaluckjantakee multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT katanchaleemaingam multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT satrawutcharoenla multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT phadungkiatkhamnoi multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT kanokwansaengsawang multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT usaneewattananandkul multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT sorasakintorasoot multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation AT khajornsaktragoolpua multifunctionalpoloxamerbasedthermoresponsivehydrogelloadedwithhumanlactoferricinniosomesinvitrostudyonantibacterialactivityacceleratewoundhealingandantiinflammation |