Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light
Background: Photothermal therapy (PTT) is one of the effective and non-invasive strategies which hold great promise for improving the treatment of cancer cells. PTT is based on activating a photosensitizer by infrared light irradiation and producing heat and reactive species and apoptosis in the tum...
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| Format: | Article |
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Shiraz University of Medical Sciences
2024-12-01
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| Series: | Journal of Biomedical Physics and Engineering |
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| Online Access: | https://jbpe.sums.ac.ir/article_50038_e8532a4b4c77ee3e8959d3a85a5989a0.pdf |
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| author | Naghmeh Sattarahmady Zahra Kayani Hossein Heli Parsa Faghani-Eskandarkolaei Hanieh Haghighi |
| author_facet | Naghmeh Sattarahmady Zahra Kayani Hossein Heli Parsa Faghani-Eskandarkolaei Hanieh Haghighi |
| author_sort | Naghmeh Sattarahmady |
| collection | DOAJ |
| description | Background: Photothermal therapy (PTT) is one of the effective and non-invasive strategies which hold great promise for improving the treatment of cancer cells. PTT is based on activating a photosensitizer by infrared light irradiation and producing heat and reactive species and apoptosis in the tumor area.Objective: The aim of this study was to investigate the effect of photothermal/chemotherapy on melanoma cancer cells using poly (2-amino phenol)/gold (P2AO/AuNPs) and doxorubicin (DOX).Material and Methods: In this experimental study, nanoparticles of P2AO/AuNPs were synthesized, and their mixture with DOX was applied as a photosensitizer for photothermal/chemotherapy of a C540 (B16-F10) melanoma cell line.Results: P2AO/AuNPs generated heat and cytotoxic responsive oxygen species (ROS) upon 808-nm light irradiation with simultaneous intensifying DOX therapeutic effect under domination of synergism effects between light irradiation, P2AO/AuNPs, and doxorubicin. Cell treatment with both P2AO/AuNPs and DOX resulted in a considerable increase in necroptotic cells to 61% with a significant decrease in the living cells (39%). Conclusion: P2AO/AuNPs provided a platform for light absorption and intensifying DOX therapeutic effect. This study approved the applicability of a new photothermal/chemotherapy by domination of synergistic effects attained by combination of laser light, P2AO, AuNPs, and DOX. |
| format | Article |
| id | doaj-art-dc285ca74e69498d94dc9540fbee45c1 |
| institution | OA Journals |
| issn | 2251-7200 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Biomedical Physics and Engineering |
| spelling | doaj-art-dc285ca74e69498d94dc9540fbee45c12025-08-20T02:35:00ZengShiraz University of Medical SciencesJournal of Biomedical Physics and Engineering2251-72002024-12-0114654756010.31661/jbpe.v0i0.2312-169350038Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm LightNaghmeh Sattarahmady0Zahra Kayani1Hossein Heli2Parsa Faghani-Eskandarkolaei3Hanieh Haghighi4Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranNanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranNanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Medical Physics and Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranNanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranBackground: Photothermal therapy (PTT) is one of the effective and non-invasive strategies which hold great promise for improving the treatment of cancer cells. PTT is based on activating a photosensitizer by infrared light irradiation and producing heat and reactive species and apoptosis in the tumor area.Objective: The aim of this study was to investigate the effect of photothermal/chemotherapy on melanoma cancer cells using poly (2-amino phenol)/gold (P2AO/AuNPs) and doxorubicin (DOX).Material and Methods: In this experimental study, nanoparticles of P2AO/AuNPs were synthesized, and their mixture with DOX was applied as a photosensitizer for photothermal/chemotherapy of a C540 (B16-F10) melanoma cell line.Results: P2AO/AuNPs generated heat and cytotoxic responsive oxygen species (ROS) upon 808-nm light irradiation with simultaneous intensifying DOX therapeutic effect under domination of synergism effects between light irradiation, P2AO/AuNPs, and doxorubicin. Cell treatment with both P2AO/AuNPs and DOX resulted in a considerable increase in necroptotic cells to 61% with a significant decrease in the living cells (39%). Conclusion: P2AO/AuNPs provided a platform for light absorption and intensifying DOX therapeutic effect. This study approved the applicability of a new photothermal/chemotherapy by domination of synergistic effects attained by combination of laser light, P2AO, AuNPs, and DOX.https://jbpe.sums.ac.ir/article_50038_e8532a4b4c77ee3e8959d3a85a5989a0.pdfmetal nanoparticlesphototherapymalignant melanomaphotoablationapoptosisdoxorubicin |
| spellingShingle | Naghmeh Sattarahmady Zahra Kayani Hossein Heli Parsa Faghani-Eskandarkolaei Hanieh Haghighi Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light Journal of Biomedical Physics and Engineering metal nanoparticles phototherapy malignant melanoma photoablation apoptosis doxorubicin |
| title | Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light |
| title_full | Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light |
| title_fullStr | Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light |
| title_full_unstemmed | Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light |
| title_short | Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light |
| title_sort | photosensitizing activity of nanoparticles of poly 2 amino phenol gold for intensified doxorubicin therapeutic effect on melanoma cancer cells under synergism effect of 808 nm light |
| topic | metal nanoparticles phototherapy malignant melanoma photoablation apoptosis doxorubicin |
| url | https://jbpe.sums.ac.ir/article_50038_e8532a4b4c77ee3e8959d3a85a5989a0.pdf |
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