Cloning and transformation of rice gene Os992 and its sensitivity to glucose
A rice (Oryza sativa) transcription factor Os922 gene of AP2/EREBP species was cloned by PCR from rice cDNA library, and constructed CoU-Os922 overexpression vector. The vector was transformed into rice Xiushui11 calli by Agrobacterium-mediated method under light co-culture conditions, and obtained...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2007-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0602 |
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| Summary: | A rice (Oryza sativa) transcription factor Os922 gene of AP2/EREBP species was cloned by PCR from rice cDNA library, and constructed CoU-Os922 overexpression vector. The vector was transformed into rice Xiushui11 calli by Agrobacterium-mediated method under light co-culture conditions, and obtained transgenic plants. Analyzing hygromycin-resistant plants by PCR and Southern blot method confirmed that Os922 was integrated into rice genome. The expression of Os922 in T<sub>1</sub> transgenic plants showed the root development was restrained by glucose, and this sensitivity to glucose suggested that Os922 may be relative to the sugar signal transduction. |
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| ISSN: | 1008-9209 2097-5155 |