Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions
This field experiment was established in Eastern Cameroon to examine the effect of selected rhizobial inoculation on N2-fixation and growth of Pueraria phaseoloides. Treatments consisted of noninoculated and Bradyrhizobium yuanmingense S3-4-inoculated Pueraria with three replications each. Ipomoea b...
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Wiley
2016-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2016/7026859 |
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| author | Papa Saliou Sarr Judith Wase Okon Didier Aime Boyogueno Begoude Shigeru Araki Zachée Ambang Makoto Shibata Shinya Funakawa |
| author_facet | Papa Saliou Sarr Judith Wase Okon Didier Aime Boyogueno Begoude Shigeru Araki Zachée Ambang Makoto Shibata Shinya Funakawa |
| author_sort | Papa Saliou Sarr |
| collection | DOAJ |
| description | This field experiment was established in Eastern Cameroon to examine the effect of selected rhizobial inoculation on N2-fixation and growth of Pueraria phaseoloides. Treatments consisted of noninoculated and Bradyrhizobium yuanmingense S3-4-inoculated Pueraria with three replications each. Ipomoea batatas as a non-N2-fixing reference was interspersed in each Pueraria plot. All the twelve plots received 2 gN/m2 of 15N ammonium sulfate 10% atom excess. At harvest, dry matter yields and the nitrogen derived from atmospheric N2-fixation (%Ndfa) of inoculated Pueraria were significantly (P<0.05) higher (81% and 10.83%, resp.) than those of noninoculated Pueraria. The inoculation enhanced nodule dry weight 2.44-fold. Consequently, the harvested N significantly (P<0.05) increased by 83% in inoculated Pueraria, resulting from the increase in N2-fixation and soil N uptake. A loss of 55 to 60% of the N fertilizer was reported, and 36 to 40% of it was immobilized in soil. Here, we demonstrated that both N2-fixing potential of P. phaseoloides and soil N uptake are improved through field inoculations using efficient bradyrhizobial species. In practice, the inoculation contributes to maximize N input in soils by the cover crop’s biomass and represent a good strategy to improve soil fertility for subsequent cultivation. |
| format | Article |
| id | doaj-art-dc15ae6859d74dfca3c3dffb9550e26b |
| institution | DOAJ |
| issn | 2090-908X |
| language | English |
| publishDate | 2016-01-01 |
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| spelling | doaj-art-dc15ae6859d74dfca3c3dffb9550e26b2025-08-20T03:23:57ZengWileyScientifica2090-908X2016-01-01201610.1155/2016/70268597026859Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field ConditionsPapa Saliou Sarr0Judith Wase Okon1Didier Aime Boyogueno Begoude2Shigeru Araki3Zachée Ambang4Makoto Shibata5Shinya Funakawa6Center for African Area Studies, Graduate School of Asian and African Area Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, JapanDepartment of Plant Biology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonRegional Biocontrol and Applied Microbiology Laboratory, Institute of Agricultural Research for Development (IRAD), P.O. Box 2067, Yaoundé, CameroonCenter for African Area Studies, Graduate School of Asian and African Area Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, JapanDepartment of Plant Biology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonGraduate School of Agriculture, Soil Science Laboratory, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, JapanGraduate School of Agriculture, Soil Science Laboratory, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, JapanThis field experiment was established in Eastern Cameroon to examine the effect of selected rhizobial inoculation on N2-fixation and growth of Pueraria phaseoloides. Treatments consisted of noninoculated and Bradyrhizobium yuanmingense S3-4-inoculated Pueraria with three replications each. Ipomoea batatas as a non-N2-fixing reference was interspersed in each Pueraria plot. All the twelve plots received 2 gN/m2 of 15N ammonium sulfate 10% atom excess. At harvest, dry matter yields and the nitrogen derived from atmospheric N2-fixation (%Ndfa) of inoculated Pueraria were significantly (P<0.05) higher (81% and 10.83%, resp.) than those of noninoculated Pueraria. The inoculation enhanced nodule dry weight 2.44-fold. Consequently, the harvested N significantly (P<0.05) increased by 83% in inoculated Pueraria, resulting from the increase in N2-fixation and soil N uptake. A loss of 55 to 60% of the N fertilizer was reported, and 36 to 40% of it was immobilized in soil. Here, we demonstrated that both N2-fixing potential of P. phaseoloides and soil N uptake are improved through field inoculations using efficient bradyrhizobial species. In practice, the inoculation contributes to maximize N input in soils by the cover crop’s biomass and represent a good strategy to improve soil fertility for subsequent cultivation.http://dx.doi.org/10.1155/2016/7026859 |
| spellingShingle | Papa Saliou Sarr Judith Wase Okon Didier Aime Boyogueno Begoude Shigeru Araki Zachée Ambang Makoto Shibata Shinya Funakawa Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions Scientifica |
| title | Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions |
| title_full | Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions |
| title_fullStr | Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions |
| title_full_unstemmed | Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions |
| title_short | Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions |
| title_sort | symbiotic n2 fixation estimated by the 15n tracer technique and growth of pueraria phaseoloides roxb benth inoculated with bradyrhizobium strain in field conditions |
| url | http://dx.doi.org/10.1155/2016/7026859 |
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