The Antioxidative Properties of Selected Herbs Estimated Using Various Assays

Antioxidants can be determined using a variety of analytical assays. The total of antioxidant properties, depending on the sum of the products of their concentrations and antioxidant powers in the sample, is usually called total antioxidant potential (TAP). The direct method developed by us, based o...

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Main Authors: Bronisław K. Głód, Mateusz Borkowski
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2023/5497076
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author Bronisław K. Głód
Mateusz Borkowski
author_facet Bronisław K. Głód
Mateusz Borkowski
author_sort Bronisław K. Głód
collection DOAJ
description Antioxidants can be determined using a variety of analytical assays. The total of antioxidant properties, depending on the sum of the products of their concentrations and antioxidant powers in the sample, is usually called total antioxidant potential (TAP). The direct method developed by us, based on the voltammetric measurements, was used to determine the TAPs of selected herbs. However, it is expected that the detection limit of this assay will be much lower when the convection current is increased and the capacitive current is eliminated. Such conditions are found in electrochemical HPLC detection. The advantage of this method is the ability to perform measurements at different potentials, which is analogous to the measurements related to various radicals in indirect methods. Measurements performed at high potentials of the working electrode allow us to test weak antioxidants. It turned out that electrochemical methods provided additional information about the antioxidant properties of the herbs. Some of them have a large part of TAP caused by weak antioxidants in high concentrations. The obtained results were correlated with the TAP values related to DPPH (2, 2-diphenyl-1-picrylhydrazyl) radicals and the total content of polyphenols in the sample.
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spelling doaj-art-dc126e7ae697485c8f41b5322b5c7cdf2025-08-20T03:23:57ZengWileyJournal of Chemistry2090-90712023-01-01202310.1155/2023/5497076The Antioxidative Properties of Selected Herbs Estimated Using Various AssaysBronisław K. Głód0Mateusz Borkowski1Siedlce University of Natural Sciences and HumanitiesSiedlce University of Natural Sciences and HumanitiesAntioxidants can be determined using a variety of analytical assays. The total of antioxidant properties, depending on the sum of the products of their concentrations and antioxidant powers in the sample, is usually called total antioxidant potential (TAP). The direct method developed by us, based on the voltammetric measurements, was used to determine the TAPs of selected herbs. However, it is expected that the detection limit of this assay will be much lower when the convection current is increased and the capacitive current is eliminated. Such conditions are found in electrochemical HPLC detection. The advantage of this method is the ability to perform measurements at different potentials, which is analogous to the measurements related to various radicals in indirect methods. Measurements performed at high potentials of the working electrode allow us to test weak antioxidants. It turned out that electrochemical methods provided additional information about the antioxidant properties of the herbs. Some of them have a large part of TAP caused by weak antioxidants in high concentrations. The obtained results were correlated with the TAP values related to DPPH (2, 2-diphenyl-1-picrylhydrazyl) radicals and the total content of polyphenols in the sample.http://dx.doi.org/10.1155/2023/5497076
spellingShingle Bronisław K. Głód
Mateusz Borkowski
The Antioxidative Properties of Selected Herbs Estimated Using Various Assays
Journal of Chemistry
title The Antioxidative Properties of Selected Herbs Estimated Using Various Assays
title_full The Antioxidative Properties of Selected Herbs Estimated Using Various Assays
title_fullStr The Antioxidative Properties of Selected Herbs Estimated Using Various Assays
title_full_unstemmed The Antioxidative Properties of Selected Herbs Estimated Using Various Assays
title_short The Antioxidative Properties of Selected Herbs Estimated Using Various Assays
title_sort antioxidative properties of selected herbs estimated using various assays
url http://dx.doi.org/10.1155/2023/5497076
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