A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves
Abstract Trichome patterning in Arabidopsis serves as a model system for de novo pattern formation in plants. It is thought to typify the theoretical activator–inhibitor mechanism, although this hypothesis has never been challenged by a combined experimental and theoretical approach. By integrating...
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| Language: | English |
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Springer Nature
2008-09-01
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| Series: | Molecular Systems Biology |
| Online Access: | https://doi.org/10.1038/msb.2008.54 |
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| _version_ | 1849225800599994368 |
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| author | Simona Digiuni Swen Schellmann Florian Geier Bettina Greese Martina Pesch Katja Wester Burcu Dartan Valerie Mach Bhylahalli Purushottam Srinivas Jens Timmer Christian Fleck Martin Hulskamp |
| author_facet | Simona Digiuni Swen Schellmann Florian Geier Bettina Greese Martina Pesch Katja Wester Burcu Dartan Valerie Mach Bhylahalli Purushottam Srinivas Jens Timmer Christian Fleck Martin Hulskamp |
| author_sort | Simona Digiuni |
| collection | DOAJ |
| description | Abstract Trichome patterning in Arabidopsis serves as a model system for de novo pattern formation in plants. It is thought to typify the theoretical activator–inhibitor mechanism, although this hypothesis has never been challenged by a combined experimental and theoretical approach. By integrating the key genetic and molecular data of the trichome patterning system, we developed a new theoretical model that allows the direct testing of the effect of experimental interventions and in the prediction of patterning phenotypes. We show experimentally that the trichome inhibitor TRIPTYCHON is transcriptionally activated by the known positive regulators GLABRA1 and GLABRA3. Further, we demonstrate by particle bombardment of protein fusions with GFP that TRIPTYCHON and CAPRICE but not GLABRA1 and GLABRA3 can move between cells. Finally, theoretical considerations suggest promoter swapping and basal overexpression experiments by means of which we are able to discriminate three biologically meaningful variants of the trichome patterning model. Our study demonstrates that the mutual interplay between theory and experiment can reveal a new level of understanding of how biochemical mechanisms can drive biological patterning processes. |
| format | Article |
| id | doaj-art-dc0f6179975a4a13bf9c1cfa3a3349cf |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2008-09-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-dc0f6179975a4a13bf9c1cfa3a3349cf2025-08-24T12:01:30ZengSpringer NatureMolecular Systems Biology1744-42922008-09-014111110.1038/msb.2008.54A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leavesSimona Digiuni0Swen Schellmann1Florian Geier2Bettina Greese3Martina Pesch4Katja Wester5Burcu Dartan6Valerie Mach7Bhylahalli Purushottam Srinivas8Jens Timmer9Christian Fleck10Martin Hulskamp11Department of Botany III, Botanical Institute, University of CologneDepartment of Botany III, Botanical Institute, University of CologneDepartment of Mathematics and Physics, University of FreiburgDepartment of Mathematics and Physics, University of FreiburgDepartment of Botany III, Botanical Institute, University of CologneDepartment of Botany III, Botanical Institute, University of CologneDepartment of Botany III, Botanical Institute, University of CologneDepartment of Botany III, Botanical Institute, University of CologneDepartment of Botany III, Botanical Institute, University of CologneDepartment of Mathematics and Physics, University of FreiburgDepartment of Mathematics and Physics, University of FreiburgDepartment of Botany III, Botanical Institute, University of CologneAbstract Trichome patterning in Arabidopsis serves as a model system for de novo pattern formation in plants. It is thought to typify the theoretical activator–inhibitor mechanism, although this hypothesis has never been challenged by a combined experimental and theoretical approach. By integrating the key genetic and molecular data of the trichome patterning system, we developed a new theoretical model that allows the direct testing of the effect of experimental interventions and in the prediction of patterning phenotypes. We show experimentally that the trichome inhibitor TRIPTYCHON is transcriptionally activated by the known positive regulators GLABRA1 and GLABRA3. Further, we demonstrate by particle bombardment of protein fusions with GFP that TRIPTYCHON and CAPRICE but not GLABRA1 and GLABRA3 can move between cells. Finally, theoretical considerations suggest promoter swapping and basal overexpression experiments by means of which we are able to discriminate three biologically meaningful variants of the trichome patterning model. Our study demonstrates that the mutual interplay between theory and experiment can reveal a new level of understanding of how biochemical mechanisms can drive biological patterning processes.https://doi.org/10.1038/msb.2008.54 |
| spellingShingle | Simona Digiuni Swen Schellmann Florian Geier Bettina Greese Martina Pesch Katja Wester Burcu Dartan Valerie Mach Bhylahalli Purushottam Srinivas Jens Timmer Christian Fleck Martin Hulskamp A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves Molecular Systems Biology |
| title | A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves |
| title_full | A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves |
| title_fullStr | A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves |
| title_full_unstemmed | A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves |
| title_short | A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves |
| title_sort | competitive complex formation mechanism underlies trichome patterning on arabidopsis leaves |
| url | https://doi.org/10.1038/msb.2008.54 |
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