Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids
Abstract There have been several reports of skin manifestations in patients with coronavirus disease 2019 (COVID-19). However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected on the skin surface, including the sebum, of these patients. In this s...
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Nature Portfolio
2024-10-01
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| Online Access: | https://doi.org/10.1038/s41598-024-77862-0 |
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| author | Tetsuya Kuwano Takayuki Kanno Minoru Tobiume Yuichiro Hirata Harutaka Katano Michiko Koga Hiroyuki Nagai Takeya Tsutsumi Noritada Yoshikawa Hiroshi Yotsuyanagi Satoshi Kutsuna Yusuke Miyazato Noriko Kinoshita-Iwamoto Norio Ohmagari Taiichiro Kobayashi Kazuaki Fukushima Masaru Tanaka Akifumi Imamura Yui Ueda Maeko Iwamura Naoto Takada Takayoshi Inoue Tetsuro Matano Ai Kawana-Tachikawa Tadaki Suzuki |
| author_facet | Tetsuya Kuwano Takayuki Kanno Minoru Tobiume Yuichiro Hirata Harutaka Katano Michiko Koga Hiroyuki Nagai Takeya Tsutsumi Noritada Yoshikawa Hiroshi Yotsuyanagi Satoshi Kutsuna Yusuke Miyazato Noriko Kinoshita-Iwamoto Norio Ohmagari Taiichiro Kobayashi Kazuaki Fukushima Masaru Tanaka Akifumi Imamura Yui Ueda Maeko Iwamura Naoto Takada Takayoshi Inoue Tetsuro Matano Ai Kawana-Tachikawa Tadaki Suzuki |
| author_sort | Tetsuya Kuwano |
| collection | DOAJ |
| description | Abstract There have been several reports of skin manifestations in patients with coronavirus disease 2019 (COVID-19). However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected on the skin surface, including the sebum, of these patients. In this study, SARS-CoV-2 RNA was detected using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay of skin surface lipids (SSLs) collected using an oil-blotting film from the faces of hospitalized patients with COVID-19. Human transcriptome analysis was also performed using the same samples. In facial SSLs of patients with COVID-19, the RT-PCR positivity rate was 84.6% (11/13 samples) within 5 days and 30.4% (7/23 samples) by 6–10 days of symptom onset. In the transcriptome analysis, the most characteristic SSL-RNA profile was the upregulation of interferon-stimulated gene (ISG)-related genes, such as ISG15, IFITM1, and MX1. This study presents an alternative technique using SSLs for non-invasive SARS-CoV-2 RNA detection and simultaneous analysis of human molecular pathogenesis in patients with COVID-19. |
| format | Article |
| id | doaj-art-dc046c1a8d294e0e859853be8a8c1d8e |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-dc046c1a8d294e0e859853be8a8c1d8e2025-08-20T02:18:28ZengNature PortfolioScientific Reports2045-23222024-10-011411810.1038/s41598-024-77862-0Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipidsTetsuya Kuwano0Takayuki Kanno1Minoru Tobiume2Yuichiro Hirata3Harutaka Katano4Michiko Koga5Hiroyuki Nagai6Takeya Tsutsumi7Noritada Yoshikawa8Hiroshi Yotsuyanagi9Satoshi Kutsuna10Yusuke Miyazato11Noriko Kinoshita-Iwamoto12Norio Ohmagari13Taiichiro Kobayashi14Kazuaki Fukushima15Masaru Tanaka16Akifumi Imamura17Yui Ueda18Maeko Iwamura19Naoto Takada20Takayoshi Inoue21Tetsuro Matano22Ai Kawana-Tachikawa23Tadaki Suzuki24Biological Science Research, Kao CorporationDepartment of Pathology, National Institute of Infectious DiseasesDepartment of Pathology, National Institute of Infectious DiseasesDepartment of Pathology, National Institute of Infectious DiseasesDepartment of Pathology, National Institute of Infectious DiseasesDivision of Infectious Diseases, Advanced Clinical Research Center, University of TokyoDepartment of Infectious Diseases and Applied Immunology, Hospital of Institute of Medical Science, University of TokyoDivision of Infectious Diseases, Advanced Clinical Research Center, University of TokyoDepartment of Allergy and Rheumatology, Institute of Medical Science, University of TokyoDivision of Infectious Diseases, Advanced Clinical Research Center, University of TokyoDisease Control and Prevention Center, National Center for Global Health and MedicineDisease Control and Prevention Center, National Center for Global Health and MedicineDisease Control and Prevention Center, National Center for Global Health and MedicineDisease Control and Prevention Center, National Center for Global Health and MedicineDepartment of Infectious Diseases, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome HospitalDepartment of Infectious Diseases, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome HospitalDepartment of Infectious Diseases, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome HospitalDepartment of Infectious Diseases, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome HospitalBiological Science Research, Kao CorporationBiological Science Research, Kao CorporationBiological Science Research, Kao CorporationBiological Science Research, Kao CorporationAIDS Research Center, National Institute of Infectious DiseasesAIDS Research Center, National Institute of Infectious DiseasesDepartment of Pathology, National Institute of Infectious DiseasesAbstract There have been several reports of skin manifestations in patients with coronavirus disease 2019 (COVID-19). However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected on the skin surface, including the sebum, of these patients. In this study, SARS-CoV-2 RNA was detected using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay of skin surface lipids (SSLs) collected using an oil-blotting film from the faces of hospitalized patients with COVID-19. Human transcriptome analysis was also performed using the same samples. In facial SSLs of patients with COVID-19, the RT-PCR positivity rate was 84.6% (11/13 samples) within 5 days and 30.4% (7/23 samples) by 6–10 days of symptom onset. In the transcriptome analysis, the most characteristic SSL-RNA profile was the upregulation of interferon-stimulated gene (ISG)-related genes, such as ISG15, IFITM1, and MX1. This study presents an alternative technique using SSLs for non-invasive SARS-CoV-2 RNA detection and simultaneous analysis of human molecular pathogenesis in patients with COVID-19.https://doi.org/10.1038/s41598-024-77862-0SARS-CoV-2COVID-19SkinSebumTranscriptomeSkin surface lipid |
| spellingShingle | Tetsuya Kuwano Takayuki Kanno Minoru Tobiume Yuichiro Hirata Harutaka Katano Michiko Koga Hiroyuki Nagai Takeya Tsutsumi Noritada Yoshikawa Hiroshi Yotsuyanagi Satoshi Kutsuna Yusuke Miyazato Noriko Kinoshita-Iwamoto Norio Ohmagari Taiichiro Kobayashi Kazuaki Fukushima Masaru Tanaka Akifumi Imamura Yui Ueda Maeko Iwamura Naoto Takada Takayoshi Inoue Tetsuro Matano Ai Kawana-Tachikawa Tadaki Suzuki Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids Scientific Reports SARS-CoV-2 COVID-19 Skin Sebum Transcriptome Skin surface lipid |
| title | Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids |
| title_full | Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids |
| title_fullStr | Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids |
| title_full_unstemmed | Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids |
| title_short | Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids |
| title_sort | non invasive sars cov 2 rna detection and human transcriptome analysis using skin surface lipids |
| topic | SARS-CoV-2 COVID-19 Skin Sebum Transcriptome Skin surface lipid |
| url | https://doi.org/10.1038/s41598-024-77862-0 |
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