Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids
ObjectiveTo establish a cell bank of extrahepatic cholangiocarcinoma (ECC)-derived organoids and investigate the key factors influencing the organoids generation. Methods The tumor samples from patients with portal cholangiocarcinoma (pCCA) and distal cholangiocarcinoma (dCCA) were used to isolate c...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shanghai Chinese Clinical Medicine Press Co., Ltd.
2025-08-01
|
| Series: | Zhongguo Linchuang Yixue |
| Subjects: | |
| Online Access: | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20250081 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849223006873714688 |
|---|---|
| author | Zijun GONG Jiaying LIU Kun FAN Sheng SHEN Wenqing QIU Xuanming LUO Houbao LIU |
| author_facet | Zijun GONG Jiaying LIU Kun FAN Sheng SHEN Wenqing QIU Xuanming LUO Houbao LIU |
| author_sort | Zijun GONG |
| collection | DOAJ |
| description | ObjectiveTo establish a cell bank of extrahepatic cholangiocarcinoma (ECC)-derived organoids and investigate the key factors influencing the organoids generation. Methods The tumor samples from patients with portal cholangiocarcinoma (pCCA) and distal cholangiocarcinoma (dCCA) were used to isolate cells, and these cells were cultured using three-dimensional (3D) technique to establish ECC organoids. Histological characteristics of the organoids were evaluated and identified through hematoxylin-eosin (HE) and immunohistochemistry stainings. The success rates of organoids generation from different tumor types were compared. And clinical characteristics of patients between successful and failure culture groups were compared. Results The success rates of organoids establishment from pCCA and dCCA were all low, with 42.4% (14/33), 51.9% (14/27), respectively. The tumor was larger in successful group than that in failure group (P＜0.001); there was no statistical difference in tumor differentiation status, microvascular invasion, and perineural invasion between the two groups. ConclusionsThe successful rate of ECC-derived organoids establishment is low, and larger tumor has higher successful culture rate. |
| format | Article |
| id | doaj-art-dc00edb823a244fe8cb92adca1fbb10f |
| institution | Kabale University |
| issn | 1008-6358 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Shanghai Chinese Clinical Medicine Press Co., Ltd. |
| record_format | Article |
| series | Zhongguo Linchuang Yixue |
| spelling | doaj-art-dc00edb823a244fe8cb92adca1fbb10f2025-08-26T01:29:39ZengShanghai Chinese Clinical Medicine Press Co., Ltd.Zhongguo Linchuang Yixue1008-63582025-08-0132460460910.12025/j.issn.1008-6358.2025.2025008120250081Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoidsZijun GONG0Jiaying LIU1Kun FAN2Sheng SHEN3Wenqing QIU4Xuanming LUO5Houbao LIU6Department of Biliary Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Biliary Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of General Surgery, Shanghai Xuhui Central Hospital, Shanghai 200032, ChinaDepartment of Biliary Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of General Surgery, Shanghai Xuhui Central Hospital, Shanghai 200032, ChinaDepartment of General Surgery, Shanghai Xuhui Central Hospital, Shanghai 200032, ChinaDepartment of Biliary Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaObjectiveTo establish a cell bank of extrahepatic cholangiocarcinoma (ECC)-derived organoids and investigate the key factors influencing the organoids generation. Methods The tumor samples from patients with portal cholangiocarcinoma (pCCA) and distal cholangiocarcinoma (dCCA) were used to isolate cells, and these cells were cultured using three-dimensional (3D) technique to establish ECC organoids. Histological characteristics of the organoids were evaluated and identified through hematoxylin-eosin (HE) and immunohistochemistry stainings. The success rates of organoids generation from different tumor types were compared. And clinical characteristics of patients between successful and failure culture groups were compared. Results The success rates of organoids establishment from pCCA and dCCA were all low, with 42.4% (14/33), 51.9% (14/27), respectively. The tumor was larger in successful group than that in failure group (P＜0.001); there was no statistical difference in tumor differentiation status, microvascular invasion, and perineural invasion between the two groups. ConclusionsThe successful rate of ECC-derived organoids establishment is low, and larger tumor has higher successful culture rate.https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20250081extrahepatic cholangiocarcinomaorganoidculture success rateinfluence factor |
| spellingShingle | Zijun GONG Jiaying LIU Kun FAN Sheng SHEN Wenqing QIU Xuanming LUO Houbao LIU Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids Zhongguo Linchuang Yixue extrahepatic cholangiocarcinoma organoid culture success rate influence factor |
| title | Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids |
| title_full | Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids |
| title_fullStr | Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids |
| title_full_unstemmed | Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids |
| title_short | Establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids |
| title_sort | establishment and related factors analysis of extrahepatic cholangiocarcinoma organoids |
| topic | extrahepatic cholangiocarcinoma organoid culture success rate influence factor |
| url | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20250081 |
| work_keys_str_mv | AT zijungong establishmentandrelatedfactorsanalysisofextrahepaticcholangiocarcinomaorganoids AT jiayingliu establishmentandrelatedfactorsanalysisofextrahepaticcholangiocarcinomaorganoids AT kunfan establishmentandrelatedfactorsanalysisofextrahepaticcholangiocarcinomaorganoids AT shengshen establishmentandrelatedfactorsanalysisofextrahepaticcholangiocarcinomaorganoids AT wenqingqiu establishmentandrelatedfactorsanalysisofextrahepaticcholangiocarcinomaorganoids AT xuanmingluo establishmentandrelatedfactorsanalysisofextrahepaticcholangiocarcinomaorganoids AT houbaoliu establishmentandrelatedfactorsanalysisofextrahepaticcholangiocarcinomaorganoids |