Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace
Apple pomace (AP) is rich in xyloglucan, serves as a substrate to produce xylogluco-oligosaccharides with various functional activities. The hydrolysis of AP by novel xyloglucanases is conducive to the high-value utilization of AP. In this study, a novel GH74 xyloglucanase gene (PbXEG74B) from Paeni...
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The editorial department of Science and Technology of Food Industry
2025-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024060437 |
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| author | Jiaxin TIAN Hang YANG Yuqi ZHAO Qiaojuan YAN Yanxiao LI Zhengqiang JIANG |
| author_facet | Jiaxin TIAN Hang YANG Yuqi ZHAO Qiaojuan YAN Yanxiao LI Zhengqiang JIANG |
| author_sort | Jiaxin TIAN |
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| description | Apple pomace (AP) is rich in xyloglucan, serves as a substrate to produce xylogluco-oligosaccharides with various functional activities. The hydrolysis of AP by novel xyloglucanases is conducive to the high-value utilization of AP. In this study, a novel GH74 xyloglucanase gene (PbXEG74B) from Paenibacillus borealis was cloned and expressed in Escherichia coli BL21 (DE3). The enzyme was characterized and further used to hydrolyze AP for xylogluco-oligosaccharides production. It was expressed in E. coli with an expression level of 18.0 U/mL. The optimal conditions of PbXEG74B were pH5.5 and 55 ℃. It showed good stability in the pH range of 4.5~8.5 and temperatures up to 40 ℃. The enzyme hydrolyzed xyloglucan to produce xylogluco-oligosaccharides with degrees of polymerization (DP) of 2~9. AP was pretreated with deep eutectic solvents (DESs) and hydrolyzed by PbXEG74B to produce xylogluco-oligosaccharides. Under optimal conditions, the yield of xylogluco-oligosaccharides with DP 2~9 was 2.79 g/100 g AP, containing 67.0%, 11.5%, and 21.5% of xylogluco-oligosaccharides with DP 2~4, 5~6, and 7~9, respectively. PbXEG74B with good enzymatic and hydrolytic properties shows great potential in the high value utilization of AP. |
| format | Article |
| id | doaj-art-dbe8ba4e1b074342a357a47de6e5a8a1 |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-dbe8ba4e1b074342a357a47de6e5a8a12025-08-20T01:55:34ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-01461114215010.13386/j.issn1002-0306.20240604372024060437-11Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple PomaceJiaxin TIAN0Hang YANG1Yuqi ZHAO2Qiaojuan YAN3Yanxiao LI4Zhengqiang JIANG5Key Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaApple pomace (AP) is rich in xyloglucan, serves as a substrate to produce xylogluco-oligosaccharides with various functional activities. The hydrolysis of AP by novel xyloglucanases is conducive to the high-value utilization of AP. In this study, a novel GH74 xyloglucanase gene (PbXEG74B) from Paenibacillus borealis was cloned and expressed in Escherichia coli BL21 (DE3). The enzyme was characterized and further used to hydrolyze AP for xylogluco-oligosaccharides production. It was expressed in E. coli with an expression level of 18.0 U/mL. The optimal conditions of PbXEG74B were pH5.5 and 55 ℃. It showed good stability in the pH range of 4.5~8.5 and temperatures up to 40 ℃. The enzyme hydrolyzed xyloglucan to produce xylogluco-oligosaccharides with degrees of polymerization (DP) of 2~9. AP was pretreated with deep eutectic solvents (DESs) and hydrolyzed by PbXEG74B to produce xylogluco-oligosaccharides. Under optimal conditions, the yield of xylogluco-oligosaccharides with DP 2~9 was 2.79 g/100 g AP, containing 67.0%, 11.5%, and 21.5% of xylogluco-oligosaccharides with DP 2~4, 5~6, and 7~9, respectively. PbXEG74B with good enzymatic and hydrolytic properties shows great potential in the high value utilization of AP.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024060437paenibacillus borealisxyloglucanaseapple pomacexylogluco-oligosaccharidedeep eutectic solvents |
| spellingShingle | Jiaxin TIAN Hang YANG Yuqi ZHAO Qiaojuan YAN Yanxiao LI Zhengqiang JIANG Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace Shipin gongye ke-ji paenibacillus borealis xyloglucanase apple pomace xylogluco-oligosaccharide deep eutectic solvents |
| title | Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace |
| title_full | Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace |
| title_fullStr | Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace |
| title_full_unstemmed | Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace |
| title_short | Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace |
| title_sort | expression and characterization of xyloglucanase from paenibacillus borealis and its application in the production of xylogluco oligosaccharides from apple pomace |
| topic | paenibacillus borealis xyloglucanase apple pomace xylogluco-oligosaccharide deep eutectic solvents |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024060437 |
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