Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV
Abstract Peste des petits ruminants (PPR), caused by the peste des petits ruminants virus (PPRV), is a highly contagious disease affecting ruminants. While goats and sheep are well-known hosts, PPRV has also spread to wild ruminants, and it remains unclear which ruminant species can be infected. SLA...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
|
| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-025-01489-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850094867200344064 |
|---|---|
| author | Xi Wei Kejia Lu Zhengwu Chang Hanwei Guo Qinfeng Li Binxuan Yuan Chen Liu Zengqi Yang Haijin Liu |
| author_facet | Xi Wei Kejia Lu Zhengwu Chang Hanwei Guo Qinfeng Li Binxuan Yuan Chen Liu Zengqi Yang Haijin Liu |
| author_sort | Xi Wei |
| collection | DOAJ |
| description | Abstract Peste des petits ruminants (PPR), caused by the peste des petits ruminants virus (PPRV), is a highly contagious disease affecting ruminants. While goats and sheep are well-known hosts, PPRV has also spread to wild ruminants, and it remains unclear which ruminant species can be infected. SLAM (Signaling lymphocytic activation molecule) acts as the primary receptor for PPRV, playing a crucial role in the viral infection process. Identifying which ruminant SLAMs can mediate PPRV infection is essential for understanding the potential hosts of PPRV, which is vital for effective eradication efforts. In this study, we first extracted 77 ruminant species’ SLAM sequences from ruminant genome database. Based on these sequences, we predicted the structures of ruminant SLAMs. The analysis revealed that SLAM conformation is similar across ruminant species, and the potential PPRV H protein binding domain residues were conserved among SLAMs of these 77 species. Phylogenetic analysis of SLAM grouped ruminants into six families. We then selected representative SLAMs from each ruminant family to assess their role in PPRV infection. Our findings demonstrated that ruminant SLAMs efficiently mediated PPRV infection, with enhanced viral amplification observed in cells expressing SLAM from java mouse deer (Tragulidae) and goat (Bovidae), compared to cells expressing SLAM from white tailed deer (Cervidae) and giraffe (Giraffidae). These results underscore the need to consider a broader range of potential host populations beyond goat and sheep in efforts to prevent and eradicate PPRV. |
| format | Article |
| id | doaj-art-dbe6c24218cd4687acc222be94a31aef |
| institution | DOAJ |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-dbe6c24218cd4687acc222be94a31aef2025-08-20T02:41:33ZengBMCVeterinary Research1297-97162025-03-0156111310.1186/s13567-025-01489-wGenetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRVXi Wei0Kejia Lu1Zhengwu Chang2Hanwei Guo3Qinfeng Li4Binxuan Yuan5Chen Liu6Zengqi Yang7Haijin Liu8College of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityAbstract Peste des petits ruminants (PPR), caused by the peste des petits ruminants virus (PPRV), is a highly contagious disease affecting ruminants. While goats and sheep are well-known hosts, PPRV has also spread to wild ruminants, and it remains unclear which ruminant species can be infected. SLAM (Signaling lymphocytic activation molecule) acts as the primary receptor for PPRV, playing a crucial role in the viral infection process. Identifying which ruminant SLAMs can mediate PPRV infection is essential for understanding the potential hosts of PPRV, which is vital for effective eradication efforts. In this study, we first extracted 77 ruminant species’ SLAM sequences from ruminant genome database. Based on these sequences, we predicted the structures of ruminant SLAMs. The analysis revealed that SLAM conformation is similar across ruminant species, and the potential PPRV H protein binding domain residues were conserved among SLAMs of these 77 species. Phylogenetic analysis of SLAM grouped ruminants into six families. We then selected representative SLAMs from each ruminant family to assess their role in PPRV infection. Our findings demonstrated that ruminant SLAMs efficiently mediated PPRV infection, with enhanced viral amplification observed in cells expressing SLAM from java mouse deer (Tragulidae) and goat (Bovidae), compared to cells expressing SLAM from white tailed deer (Cervidae) and giraffe (Giraffidae). These results underscore the need to consider a broader range of potential host populations beyond goat and sheep in efforts to prevent and eradicate PPRV.https://doi.org/10.1186/s13567-025-01489-wPPRVSLAMruminant animalshost range |
| spellingShingle | Xi Wei Kejia Lu Zhengwu Chang Hanwei Guo Qinfeng Li Binxuan Yuan Chen Liu Zengqi Yang Haijin Liu Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV Veterinary Research PPRV SLAM ruminant animals host range |
| title | Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV |
| title_full | Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV |
| title_fullStr | Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV |
| title_full_unstemmed | Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV |
| title_short | Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV |
| title_sort | genetic analyses and functional validation of ruminant slams reveal potential hosts for pprv |
| topic | PPRV SLAM ruminant animals host range |
| url | https://doi.org/10.1186/s13567-025-01489-w |
| work_keys_str_mv | AT xiwei geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT kejialu geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT zhengwuchang geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT hanweiguo geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT qinfengli geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT binxuanyuan geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT chenliu geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT zengqiyang geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv AT haijinliu geneticanalysesandfunctionalvalidationofruminantslamsrevealpotentialhostsforpprv |