Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential
Abstract Background Cardiolipin (CL) is a signature phospholipid of mitochondria that maintains the integrity of mitochondrial membrane and supports proper mitochondrial function. Alterations in CL level and composition can impair or, conversely, improve mitochondrial function and bioenergetics, bot...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | Lipids in Health and Disease |
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| Online Access: | https://doi.org/10.1186/s12944-025-02499-5 |
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| author | Pavels Dimitrijevs Ilona Freiliba Andrejs Pčolkins Marcis Leja Pavel Arsenyan |
| author_facet | Pavels Dimitrijevs Ilona Freiliba Andrejs Pčolkins Marcis Leja Pavel Arsenyan |
| author_sort | Pavels Dimitrijevs |
| collection | DOAJ |
| description | Abstract Background Cardiolipin (CL) is a signature phospholipid of mitochondria that maintains the integrity of mitochondrial membrane and supports proper mitochondrial function. Alterations in CL level and composition can impair or, conversely, improve mitochondrial function and bioenergetics, both of which are critical for cancer metabolism. However, conflicting reports on CL levels across different cancer types and limited research using human patient samples limit our understanding of its diagnostic potential. Methods This cross-sectional study explores CL concentrations in gastric and colon cancer tissues using a CL-specific fluorescent probe MitoCLue and compares them to adjacent healthy tissues. Results In gastric cancer, CL levels showed no significant differences between tumor and healthy tissues, suggesting that metabolic shifts in gastric cancer do not affect total CL content. In contrast, colon cancer tissues exhibited a significant 33% increase in CL levels, indicating mitochondrial adaptation and/or increase in mitochondrial mass in colon cancer. No associations were found between CL levels and patient demographic factors; although a weak correlation with body mass index was noted. Conclusion We successfully applied MitoCLue to quantitatively assess the total CL level in healthy and tumor tissues from patients with gastric or colon cancer. The distinct CL levels in gastric and colon cancer suggest that there are cancer-type specific mitochondrial adaptations, reflecting unique bioenergetic demands and metabolic reprogramming pathways. While a 33% increase in CL levels was observed in colon cancer tissues compared to healthy adjacent tissues, this modest variation may limit its utility as a standalone biomarker. |
| format | Article |
| id | doaj-art-dbd94064dee24b118e2043619693f1bb |
| institution | OA Journals |
| issn | 1476-511X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj-art-dbd94064dee24b118e2043619693f1bb2025-08-20T02:16:45ZengBMCLipids in Health and Disease1476-511X2025-02-0124111310.1186/s12944-025-02499-5Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potentialPavels Dimitrijevs0Ilona Freiliba1Andrejs Pčolkins2Marcis Leja3Pavel Arsenyan4Latvian Institute of Organic SynthesisInstitute of Clinical and Preventive Medicine, University of LatviaInstitute of Clinical and Preventive Medicine, University of LatviaInstitute of Clinical and Preventive Medicine, University of LatviaLatvian Institute of Organic SynthesisAbstract Background Cardiolipin (CL) is a signature phospholipid of mitochondria that maintains the integrity of mitochondrial membrane and supports proper mitochondrial function. Alterations in CL level and composition can impair or, conversely, improve mitochondrial function and bioenergetics, both of which are critical for cancer metabolism. However, conflicting reports on CL levels across different cancer types and limited research using human patient samples limit our understanding of its diagnostic potential. Methods This cross-sectional study explores CL concentrations in gastric and colon cancer tissues using a CL-specific fluorescent probe MitoCLue and compares them to adjacent healthy tissues. Results In gastric cancer, CL levels showed no significant differences between tumor and healthy tissues, suggesting that metabolic shifts in gastric cancer do not affect total CL content. In contrast, colon cancer tissues exhibited a significant 33% increase in CL levels, indicating mitochondrial adaptation and/or increase in mitochondrial mass in colon cancer. No associations were found between CL levels and patient demographic factors; although a weak correlation with body mass index was noted. Conclusion We successfully applied MitoCLue to quantitatively assess the total CL level in healthy and tumor tissues from patients with gastric or colon cancer. The distinct CL levels in gastric and colon cancer suggest that there are cancer-type specific mitochondrial adaptations, reflecting unique bioenergetic demands and metabolic reprogramming pathways. While a 33% increase in CL levels was observed in colon cancer tissues compared to healthy adjacent tissues, this modest variation may limit its utility as a standalone biomarker.https://doi.org/10.1186/s12944-025-02499-5Colon cancerGastric cancerCardiolipinBiomarkerDiagnostic |
| spellingShingle | Pavels Dimitrijevs Ilona Freiliba Andrejs Pčolkins Marcis Leja Pavel Arsenyan Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential Lipids in Health and Disease Colon cancer Gastric cancer Cardiolipin Biomarker Diagnostic |
| title | Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential |
| title_full | Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential |
| title_fullStr | Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential |
| title_full_unstemmed | Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential |
| title_short | Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential |
| title_sort | total cardiolipin levels in gastric and colon cancer evaluating the prognostic potential |
| topic | Colon cancer Gastric cancer Cardiolipin Biomarker Diagnostic |
| url | https://doi.org/10.1186/s12944-025-02499-5 |
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