Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy
This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial in...
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| Format: | Article |
| Language: | English |
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University of Belgrade, Technical Faculty, Bor
2012-01-01
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| Series: | Journal of Mining and Metallurgy. Section B: Metallurgy |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-5339/2012/1450-53391200016M.pdf |
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| author | Milić Jelena S. Beškoski Vladimir P. Ranđelović Danijela V. Stojanović Jelica Vrvić Miroslav M. |
| author_facet | Milić Jelena S. Beškoski Vladimir P. Ranđelović Danijela V. Stojanović Jelica Vrvić Miroslav M. |
| author_sort | Milić Jelena S. |
| collection | DOAJ |
| description | This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial interaction between the microorganisms and the surface minerals of an oil shale and to evaluate bacterial effects in the first hours of the bioleaching process. Acidithiobacillus ferrooxidans was attached to the shale surface already after 2 h, and after 48 h, numerous cells covered the surface with a biofilm. After 5 day incubation with At. ferrooxidans, AFM imaging revealed ellipsoid etched pits that represent footprints left by detached cells. Combining AFM surface imaging and leaching analysis following bacterial colonisation of oil shale layers demonstrates that an initial attachment to the surface is necessary for the leaching and that later on, once a sufficient concentration of Fe2+ ions in the solution is achieved, cells detach to become free cells, and leaching occurs primarily by the Fe3+. This experiment confirmed that microorganisms isolated from sites in which a particular substrate is found will demonstrate stronger binding to that substrate. |
| format | Article |
| id | doaj-art-dbd72a331a9e427e929580bbaf7c158a |
| institution | Kabale University |
| issn | 1450-5339 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | University of Belgrade, Technical Faculty, Bor |
| record_format | Article |
| series | Journal of Mining and Metallurgy. Section B: Metallurgy |
| spelling | doaj-art-dbd72a331a9e427e929580bbaf7c158a2025-02-02T03:09:05ZengUniversity of Belgrade, Technical Faculty, BorJournal of Mining and Metallurgy. Section B: Metallurgy1450-53392012-01-0148220721710.2298/JMMB110923016MVisualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopyMilić Jelena S.Beškoski Vladimir P.Ranđelović Danijela V.Stojanović JelicaVrvić Miroslav M.This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial interaction between the microorganisms and the surface minerals of an oil shale and to evaluate bacterial effects in the first hours of the bioleaching process. Acidithiobacillus ferrooxidans was attached to the shale surface already after 2 h, and after 48 h, numerous cells covered the surface with a biofilm. After 5 day incubation with At. ferrooxidans, AFM imaging revealed ellipsoid etched pits that represent footprints left by detached cells. Combining AFM surface imaging and leaching analysis following bacterial colonisation of oil shale layers demonstrates that an initial attachment to the surface is necessary for the leaching and that later on, once a sufficient concentration of Fe2+ ions in the solution is achieved, cells detach to become free cells, and leaching occurs primarily by the Fe3+. This experiment confirmed that microorganisms isolated from sites in which a particular substrate is found will demonstrate stronger binding to that substrate.http://www.doiserbia.nb.rs/img/doi/1450-5339/2012/1450-53391200016M.pdfatomic force microscopyAcidithiobacillus ferrooxidansbioleachingoil shale |
| spellingShingle | Milić Jelena S. Beškoski Vladimir P. Ranđelović Danijela V. Stojanović Jelica Vrvić Miroslav M. Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy Journal of Mining and Metallurgy. Section B: Metallurgy atomic force microscopy Acidithiobacillus ferrooxidans bioleaching oil shale |
| title | Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy |
| title_full | Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy |
| title_fullStr | Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy |
| title_full_unstemmed | Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy |
| title_short | Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy |
| title_sort | visualisation of the interaction between acidithiobacillus ferrooxidans and oil shale by atomic force microscopy |
| topic | atomic force microscopy Acidithiobacillus ferrooxidans bioleaching oil shale |
| url | http://www.doiserbia.nb.rs/img/doi/1450-5339/2012/1450-53391200016M.pdf |
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