A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula
Abstract The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04547-2 |
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| author | Nicolás Vidal-Vázquez Ismael Hernández-Núñez Pablo Carballo-Pacoret Sarah Salisbury Paula R. Villamayor Francisca Hervas-Sotomayor Xuefei Yuan Francesco Lamanna Céline Schneider Julia Schmidt Sylvie Mazan Henrik Kaessmann Fátima Adrio Diego Robledo Antón Barreiro-Iglesias Eva Candal |
| author_facet | Nicolás Vidal-Vázquez Ismael Hernández-Núñez Pablo Carballo-Pacoret Sarah Salisbury Paula R. Villamayor Francisca Hervas-Sotomayor Xuefei Yuan Francesco Lamanna Céline Schneider Julia Schmidt Sylvie Mazan Henrik Kaessmann Fátima Adrio Diego Robledo Antón Barreiro-Iglesias Eva Candal |
| author_sort | Nicolás Vidal-Vázquez |
| collection | DOAJ |
| description | Abstract The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing new insights on retinal evolution and development. However, similar data in cartilaginous fishes, the sister group to all other extant jawed vertebrates, are still lacking. Here, we present a single-nucleus RNA sequencing atlas of the postnatal retina of the catshark Scyliorhinus canicula, consisting of the expression profiles for 17,438 individual cells from three female, juvenile catshark specimens. Unsupervised clustering revealed 22 distinct cell types comprising all major retinal cell classes, as well as retinal progenitor cells (whose presence reflects the persistence of proliferative activity in postnatal stages in sharks) and oligodendrocytes. Thus, our dataset serves as a foundation for further studies on the development and function of the catshark retina. Moreover, integration of our atlas with data from other species will allow for a better understanding of vertebrate retinal evolution. |
| format | Article |
| id | doaj-art-dbd5d905ca98457bb49ad840b4e58014 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-dbd5d905ca98457bb49ad840b4e580142025-02-09T12:11:25ZengNature PortfolioScientific Data2052-44632025-02-0112111110.1038/s41597-025-04547-2A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus caniculaNicolás Vidal-Vázquez0Ismael Hernández-Núñez1Pablo Carballo-Pacoret2Sarah Salisbury3Paula R. Villamayor4Francisca Hervas-Sotomayor5Xuefei Yuan6Francesco Lamanna7Céline Schneider8Julia Schmidt9Sylvie Mazan10Henrik Kaessmann11Fátima Adrio12Diego Robledo13Antón Barreiro-Iglesias14Eva Candal15Departamento de Bioloxía Funcional, Facultade de Bioloxía, Universidade de Santiago de CompostelaDepartamento de Bioloxía Funcional, Facultade de Bioloxía, Universidade de Santiago de CompostelaDepartamento de Bioloxía Funcional, Facultade de Bioloxía, Universidade de Santiago de CompostelaThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityCNRS, Sorbonne Université, Biologie Intégrative des Organismes Marins, UMR7232-BIOMCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityDepartamento de Bioloxía Funcional, Facultade de Bioloxía, Universidade de Santiago de CompostelaThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghDepartamento de Bioloxía Funcional, Facultade de Bioloxía, Universidade de Santiago de CompostelaDepartamento de Bioloxía Funcional, Facultade de Bioloxía, Universidade de Santiago de CompostelaAbstract The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing new insights on retinal evolution and development. However, similar data in cartilaginous fishes, the sister group to all other extant jawed vertebrates, are still lacking. Here, we present a single-nucleus RNA sequencing atlas of the postnatal retina of the catshark Scyliorhinus canicula, consisting of the expression profiles for 17,438 individual cells from three female, juvenile catshark specimens. Unsupervised clustering revealed 22 distinct cell types comprising all major retinal cell classes, as well as retinal progenitor cells (whose presence reflects the persistence of proliferative activity in postnatal stages in sharks) and oligodendrocytes. Thus, our dataset serves as a foundation for further studies on the development and function of the catshark retina. Moreover, integration of our atlas with data from other species will allow for a better understanding of vertebrate retinal evolution.https://doi.org/10.1038/s41597-025-04547-2 |
| spellingShingle | Nicolás Vidal-Vázquez Ismael Hernández-Núñez Pablo Carballo-Pacoret Sarah Salisbury Paula R. Villamayor Francisca Hervas-Sotomayor Xuefei Yuan Francesco Lamanna Céline Schneider Julia Schmidt Sylvie Mazan Henrik Kaessmann Fátima Adrio Diego Robledo Antón Barreiro-Iglesias Eva Candal A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula Scientific Data |
| title | A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula |
| title_full | A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula |
| title_fullStr | A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula |
| title_full_unstemmed | A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula |
| title_short | A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula |
| title_sort | single nucleus rna sequencing atlas of the postnatal retina of the shark scyliorhinus canicula |
| url | https://doi.org/10.1038/s41597-025-04547-2 |
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