Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cells
Benzotriazole ultraviolet stabilizers (BUVSs) are pervasive environmental contaminants that pose significant risks to human health. This study evaluated the effects of three typical BUVSs (UV-328, UV-329, and UV-P) on human mesenchymal stem cells (hMSCs), which play crucial roles in tissue maintenan...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001010 |
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| author | Yue Zhang Hui Zhao Qianxi Feng Rui Guo Li Zhong Shengxian Liang |
| author_facet | Yue Zhang Hui Zhao Qianxi Feng Rui Guo Li Zhong Shengxian Liang |
| author_sort | Yue Zhang |
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| description | Benzotriazole ultraviolet stabilizers (BUVSs) are pervasive environmental contaminants that pose significant risks to human health. This study evaluated the effects of three typical BUVSs (UV-328, UV-329, and UV-P) on human mesenchymal stem cells (hMSCs), which play crucial roles in tissue maintenance and repair. hMSCs were exposed to BUVSs across a range of concentrations, and their maintenance and differentiation capacities were assessed. At concentrations below 50 μM, no significant cytotoxicity was observed. However, at non-cytotoxic doses, UV-P exhibited stronger effects on the differentiation of hMSCs compared to UV-328 and UV-329, significantly inhibiting adipogenesis and enhancing osteogenesis. Mechanistically, UV-P was found to significantly enrich the PPAR signaling pathway during both differentiation processes. Dual-luciferase reporter assays confirmed UV-P's interaction with PPARγ_LBD at an alternate binding site outside the canonical pocket. These findings raise concerns about the health impacts of BUVSs, particularly UV-P, and underscore the need for further investigation into their toxicological profiles. |
| format | Article |
| id | doaj-art-dbd5984e5af14e878c26fff67fa2fe83 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-dbd5984e5af14e878c26fff67fa2fe832025-02-12T05:30:16ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117765Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cellsYue Zhang0Hui Zhao1Qianxi Feng2Rui Guo3Li Zhong4Shengxian Liang5Institute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, ChinaInstitute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, ChinaInstitute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, ChinaInstitute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, ChinaInstitute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, China; Western University of Health Sciences, Pomona, CA 91766, USA; Corresponding authors at: Institute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, China.Institute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, China; Institutes of Health Central Plains, Xinxiang Medical University, Xinxiang 453003, China; Corresponding authors at: Institute of Life Sciences and Green Development, College of Life Sciences, Hebei University, Baoding 071000, China.Benzotriazole ultraviolet stabilizers (BUVSs) are pervasive environmental contaminants that pose significant risks to human health. This study evaluated the effects of three typical BUVSs (UV-328, UV-329, and UV-P) on human mesenchymal stem cells (hMSCs), which play crucial roles in tissue maintenance and repair. hMSCs were exposed to BUVSs across a range of concentrations, and their maintenance and differentiation capacities were assessed. At concentrations below 50 μM, no significant cytotoxicity was observed. However, at non-cytotoxic doses, UV-P exhibited stronger effects on the differentiation of hMSCs compared to UV-328 and UV-329, significantly inhibiting adipogenesis and enhancing osteogenesis. Mechanistically, UV-P was found to significantly enrich the PPAR signaling pathway during both differentiation processes. Dual-luciferase reporter assays confirmed UV-P's interaction with PPARγ_LBD at an alternate binding site outside the canonical pocket. These findings raise concerns about the health impacts of BUVSs, particularly UV-P, and underscore the need for further investigation into their toxicological profiles.http://www.sciencedirect.com/science/article/pii/S0147651325001010Benzotriazole ultraviolet stabilizersHuman mesenchymal stem cellsSelf-renewalAdipo-osteogenesisPPARγ |
| spellingShingle | Yue Zhang Hui Zhao Qianxi Feng Rui Guo Li Zhong Shengxian Liang Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cells Ecotoxicology and Environmental Safety Benzotriazole ultraviolet stabilizers Human mesenchymal stem cells Self-renewal Adipo-osteogenesis PPARγ |
| title | Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cells |
| title_full | Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cells |
| title_fullStr | Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cells |
| title_full_unstemmed | Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cells |
| title_short | Effects of benzotriazoles UV-328, UV-329, and UV-P on the self-renewal and adipo-osteogenic differentiation of human mesenchymal stem cells |
| title_sort | effects of benzotriazoles uv 328 uv 329 and uv p on the self renewal and adipo osteogenic differentiation of human mesenchymal stem cells |
| topic | Benzotriazole ultraviolet stabilizers Human mesenchymal stem cells Self-renewal Adipo-osteogenesis PPARγ |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001010 |
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