Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators
Fiber-photometric is a novel optogenetic method for recording neural activity in vivo, which allows the use of calcium indicators to observe and study the relationship between neural activity and behavior in free-ranging animals. Calcium indicators also convert changes in calcium concentration in ce...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Organogenesis |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15476278.2025.2489667 |
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| _version_ | 1849733311881019392 |
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| author | Lanxia Wu Wenxuan Sun Linjie Huang Lin Sun Jinhua Dou Guohua Lu |
| author_facet | Lanxia Wu Wenxuan Sun Linjie Huang Lin Sun Jinhua Dou Guohua Lu |
| author_sort | Lanxia Wu |
| collection | DOAJ |
| description | Fiber-photometric is a novel optogenetic method for recording neural activity in vivo, which allows the use of calcium indicators to observe and study the relationship between neural activity and behavior in free-ranging animals. Calcium indicators also convert changes in calcium concentration in cells or tissues into recordable fluorescent signals, which can then be observed using the system of fiber-photometric. To date, there is a paucity of relevant literature on the proper selection and application of fiber-photometric indicators. Therefore, this paper will detail how to correctly select and apply fiber-photometer indicators in four sections: the basic principle of optical fiber photometry, the selection of calcium fluorescent probes and viral vector systems, and the measurement of specific expression of fluorescent proteins in specific tissues. Therefore, the correct use of suitable fiber optic recording indicators will greatly assist researchers in exploring the link between neuronal activity and neuropsychiatric disorders. |
| format | Article |
| id | doaj-art-dbd4af3bd18049deb581dfe83c837e14 |
| institution | DOAJ |
| issn | 1547-6278 1555-8592 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Organogenesis |
| spelling | doaj-art-dbd4af3bd18049deb581dfe83c837e142025-08-20T03:08:05ZengTaylor & Francis GroupOrganogenesis1547-62781555-85922025-12-0121110.1080/15476278.2025.2489667Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric IndicatorsLanxia Wu0Wenxuan Sun1Linjie Huang2Lin Sun3Jinhua Dou4Guohua Lu5School of Psychology, Shandong Second Medical University, Weifang, Shandong, ChinaSchool of Clinical Medicine, Shandong Second Medical University, Weifang, Shandong, ChinaSchool of Clinical Medicine, Shandong Second Medical University, Weifang, Shandong, ChinaSchool of Psychology, Shandong Second Medical University, Weifang, Shandong, ChinaMental Health Education Center, Shandong Second Medical University, Weifang, Shandong, ChinaSchool of Psychology, Shandong Second Medical University, Weifang, Shandong, ChinaFiber-photometric is a novel optogenetic method for recording neural activity in vivo, which allows the use of calcium indicators to observe and study the relationship between neural activity and behavior in free-ranging animals. Calcium indicators also convert changes in calcium concentration in cells or tissues into recordable fluorescent signals, which can then be observed using the system of fiber-photometric. To date, there is a paucity of relevant literature on the proper selection and application of fiber-photometric indicators. Therefore, this paper will detail how to correctly select and apply fiber-photometer indicators in four sections: the basic principle of optical fiber photometry, the selection of calcium fluorescent probes and viral vector systems, and the measurement of specific expression of fluorescent proteins in specific tissues. Therefore, the correct use of suitable fiber optic recording indicators will greatly assist researchers in exploring the link between neuronal activity and neuropsychiatric disorders.https://www.tandfonline.com/doi/10.1080/15476278.2025.2489667Calcium imagingfibre photometrygenetically encoded calcium indicators(GECIs)virus selection |
| spellingShingle | Lanxia Wu Wenxuan Sun Linjie Huang Lin Sun Jinhua Dou Guohua Lu Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators Organogenesis Calcium imaging fibre photometry genetically encoded calcium indicators(GECIs) virus selection |
| title | Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators |
| title_full | Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators |
| title_fullStr | Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators |
| title_full_unstemmed | Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators |
| title_short | Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators |
| title_sort | calcium imaging in vivo how to correctly select and apply fiber optic photometric indicators |
| topic | Calcium imaging fibre photometry genetically encoded calcium indicators(GECIs) virus selection |
| url | https://www.tandfonline.com/doi/10.1080/15476278.2025.2489667 |
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