<i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement Development
<i>Morus alba</i> L. (Moraceae), white mulberry, is an ancient, well-known source of several compounds with potent biological activities and beneficial effects on human health. In this study, the juices of three stabilised undifferentiated cell lines, calli maintained in light and dark c...
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2025-05-01
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| author | Vanessa Dalla Costa Anna Piovan Paola Brun Raffaella Filippini |
| author_facet | Vanessa Dalla Costa Anna Piovan Paola Brun Raffaella Filippini |
| author_sort | Vanessa Dalla Costa |
| collection | DOAJ |
| description | <i>Morus alba</i> L. (Moraceae), white mulberry, is an ancient, well-known source of several compounds with potent biological activities and beneficial effects on human health. In this study, the juices of three stabilised undifferentiated cell lines, calli maintained in light and dark conditions, and suspensions maintained in dark condition of <i>M. alba</i> were investigated for their phytochemical content and biological activity. The results highlighted the main presence of oxyresveratrol and resveratrol-backbone glucosides, together with benzofuran derivatives. Oxyresveratrol triglucoside was found for the first time in <i>M</i>. <i>alba</i> in vitro cultures, where it represents the main compound, accounting for almost 90 µg/mL in all the juices. The total stilbenoid content resulted significantly higher in calli juices during the logarithmic phase of the growth cycle, and cell suspension juice exhibited the statistically highest total content (313.21 µg/mL of juice). Only cell suspension juice showed ROS reduction in Caco-2 cells, whereas all the juices reduced IL-1β and TNF-α levels in Caco-2 cells stimulated with LPS. These results lay the groundwork for the future exploitation of <i>M</i>. <i>alba</i> dedifferentiated cultures as sustainable resources of stilbenoid compounds to be used in the nutraceutical, cosmetic, and pharmaceutical industries. |
| format | Article |
| id | doaj-art-dbcbe0baa0e04c0894f58fd53f573da1 |
| institution | OA Journals |
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| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-dbcbe0baa0e04c0894f58fd53f573da12025-08-20T01:49:24ZengMDPI AGMolecules1420-30492025-05-01309207310.3390/molecules30092073<i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement DevelopmentVanessa Dalla Costa0Anna Piovan1Paola Brun2Raffaella Filippini3Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo, 5, 35131 Padua, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo, 5, 35131 Padua, ItalyDepartment of Molecular Medicine, University of Padova, Via Gabelli 63, 35121 Padova, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo, 5, 35131 Padua, Italy<i>Morus alba</i> L. (Moraceae), white mulberry, is an ancient, well-known source of several compounds with potent biological activities and beneficial effects on human health. In this study, the juices of three stabilised undifferentiated cell lines, calli maintained in light and dark conditions, and suspensions maintained in dark condition of <i>M. alba</i> were investigated for their phytochemical content and biological activity. The results highlighted the main presence of oxyresveratrol and resveratrol-backbone glucosides, together with benzofuran derivatives. Oxyresveratrol triglucoside was found for the first time in <i>M</i>. <i>alba</i> in vitro cultures, where it represents the main compound, accounting for almost 90 µg/mL in all the juices. The total stilbenoid content resulted significantly higher in calli juices during the logarithmic phase of the growth cycle, and cell suspension juice exhibited the statistically highest total content (313.21 µg/mL of juice). Only cell suspension juice showed ROS reduction in Caco-2 cells, whereas all the juices reduced IL-1β and TNF-α levels in Caco-2 cells stimulated with LPS. These results lay the groundwork for the future exploitation of <i>M</i>. <i>alba</i> dedifferentiated cultures as sustainable resources of stilbenoid compounds to be used in the nutraceutical, cosmetic, and pharmaceutical industries.https://www.mdpi.com/1420-3049/30/9/2073<i>Morus alba</i>callisuspensionsstilbenoidsanti-inflammatory activityantioxidant activity |
| spellingShingle | Vanessa Dalla Costa Anna Piovan Paola Brun Raffaella Filippini <i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement Development Molecules <i>Morus alba</i> calli suspensions stilbenoids anti-inflammatory activity antioxidant activity |
| title | <i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement Development |
| title_full | <i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement Development |
| title_fullStr | <i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement Development |
| title_full_unstemmed | <i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement Development |
| title_short | <i>Morus alba</i> L. Cell Cultures as Sources of Antioxidant and Anti-Inflammatory Stilbenoids for Food Supplement Development |
| title_sort | i morus alba i l cell cultures as sources of antioxidant and anti inflammatory stilbenoids for food supplement development |
| topic | <i>Morus alba</i> calli suspensions stilbenoids anti-inflammatory activity antioxidant activity |
| url | https://www.mdpi.com/1420-3049/30/9/2073 |
| work_keys_str_mv | AT vanessadallacosta imorusalbailcellculturesassourcesofantioxidantandantiinflammatorystilbenoidsforfoodsupplementdevelopment AT annapiovan imorusalbailcellculturesassourcesofantioxidantandantiinflammatorystilbenoidsforfoodsupplementdevelopment AT paolabrun imorusalbailcellculturesassourcesofantioxidantandantiinflammatorystilbenoidsforfoodsupplementdevelopment AT raffaellafilippini imorusalbailcellculturesassourcesofantioxidantandantiinflammatorystilbenoidsforfoodsupplementdevelopment |