Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)

Aluminum(Al) toxicity is a major constraint affecting crop growth in acidic soils across the globe. Excessive Al levels in such soils not only negatively affect crop growth but also have significant implications for human health. This study aimed to explore the feasibility of increasing tolerance to...

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Main Authors: Yingming Feng, Yuxin Zheng, Wei Nong, Xingyun Chen, Zeyan Wang, Peng Zeng, Xuewen Li, Shabala Sergey, Lei Shi, Min Yu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-03-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1516663/full
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author Yingming Feng
Yingming Feng
Yuxin Zheng
Wei Nong
Xingyun Chen
Xingyun Chen
Zeyan Wang
Peng Zeng
Xuewen Li
Shabala Sergey
Shabala Sergey
Lei Shi
Min Yu
author_facet Yingming Feng
Yingming Feng
Yuxin Zheng
Wei Nong
Xingyun Chen
Xingyun Chen
Zeyan Wang
Peng Zeng
Xuewen Li
Shabala Sergey
Shabala Sergey
Lei Shi
Min Yu
author_sort Yingming Feng
collection DOAJ
description Aluminum(Al) toxicity is a major constraint affecting crop growth in acidic soils across the globe. Excessive Al levels in such soils not only negatively affect crop growth but also have significant implications for human health. This study aimed to explore the feasibility of increasing tolerance to Al stress by creating biomineralization structures in plant roots by nano-silica, and to explore the physiological basis silicon-mediated alleviation of Al toxicity in plants. The polyethylenimine was used to induce nano-silica to form biomineralization structures on the surface of root tip and root border cells in pea (Pisum sativum) plants. The results showed that under Al stress conditions, the deposition of nano-silica on the cell wall of pea root border cells induced by polyethyleneimine effectively increased cell viability and reduced reactive oxygen species(ROS) production by 44%, thus slowing down the programmed cell death. Such deposition also resulted in more Al ions(Al3+) absorbed by the surface of the root tip, thus preventing Al3+ from entering the root tip and alleviating the toxic effects of Al on cell metabolism. It is concluded that polyethylenimine- induced nano-silica deposition on the cell wall endows pea root cells with Al tolerance, thus enhancing crop growth and reducing toxic Al load, contributing to food safety and human health.
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issn 1664-462X
language English
publishDate 2025-03-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Plant Science
spelling doaj-art-dbc98d31ec6c4a9786bae1c0c130d1232025-08-20T02:47:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-03-011610.3389/fpls.2025.15166631516663Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)Yingming Feng0Yingming Feng1Yuxin Zheng2Wei Nong3Xingyun Chen4Xingyun Chen5Zeyan Wang6Peng Zeng7Xuewen Li8Shabala Sergey9Shabala Sergey10Lei Shi11Min Yu12National Key Lab of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, Wuhan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaSchool of Biological Science, University of Western Australia, Crawley, WA, AustraliaDepartment of Educational Information Technology, Foshan University, Foshan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaSchool of Biological Science, University of Western Australia, Crawley, WA, AustraliaNational Key Lab of Crop Genetic Improvement, Microelement Research Centre, Huazhong Agricultural University, Wuhan, ChinaInternational Research Center for Environmental Membrane Biology, School of Agricultural and Bioengineering, Foshan University, Foshan, ChinaAluminum(Al) toxicity is a major constraint affecting crop growth in acidic soils across the globe. Excessive Al levels in such soils not only negatively affect crop growth but also have significant implications for human health. This study aimed to explore the feasibility of increasing tolerance to Al stress by creating biomineralization structures in plant roots by nano-silica, and to explore the physiological basis silicon-mediated alleviation of Al toxicity in plants. The polyethylenimine was used to induce nano-silica to form biomineralization structures on the surface of root tip and root border cells in pea (Pisum sativum) plants. The results showed that under Al stress conditions, the deposition of nano-silica on the cell wall of pea root border cells induced by polyethyleneimine effectively increased cell viability and reduced reactive oxygen species(ROS) production by 44%, thus slowing down the programmed cell death. Such deposition also resulted in more Al ions(Al3+) absorbed by the surface of the root tip, thus preventing Al3+ from entering the root tip and alleviating the toxic effects of Al on cell metabolism. It is concluded that polyethylenimine- induced nano-silica deposition on the cell wall endows pea root cells with Al tolerance, thus enhancing crop growth and reducing toxic Al load, contributing to food safety and human health.https://www.frontiersin.org/articles/10.3389/fpls.2025.1516663/fullpeapolyethyleneiminenano-silicaaluminum toxicityroot tipsroot border cells
spellingShingle Yingming Feng
Yingming Feng
Yuxin Zheng
Wei Nong
Xingyun Chen
Xingyun Chen
Zeyan Wang
Peng Zeng
Xuewen Li
Shabala Sergey
Shabala Sergey
Lei Shi
Min Yu
Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)
Frontiers in Plant Science
pea
polyethyleneimine
nano-silica
aluminum toxicity
root tips
root border cells
title Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)
title_full Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)
title_fullStr Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)
title_full_unstemmed Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)
title_short Physiological basis of nano-silica deposition-related improvement in aluminum tolerance in pea (Pisum sativum)
title_sort physiological basis of nano silica deposition related improvement in aluminum tolerance in pea pisum sativum
topic pea
polyethyleneimine
nano-silica
aluminum toxicity
root tips
root border cells
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1516663/full
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