Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard.

Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard.

We have developed a very simple method, termed absorbance summation (AS), for comparing protein concentrations between samples in ELISA assays without a standard. This method sums the observed absorbance values from all dilutions to obtain one data point for each sample to be used for comparison. AS...

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Bibliographic Details
Main Authors: Holly Hartman, Yuge Wang, Harry W Schroeder, Xiangqin Cui
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198528/1/pone.0198528.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T113251Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=19003708b7942361a90aa7588a4ed7e24dca0beaea403d0898722e3bbdb4b7647c684100c0799d9bab5d3aeb724b6c89fb1786a8d1fc3ab6f9f905de12a8681d2db03b89e217cc11821f8fcd54d522975b3e3919502a715631f9459284db5f995975cf96062fe3cf87373af564e234bb3786af67c5ddb675f983848359aaaaecd380608d81baa41705d7772baf170d3b85164d273af41c18c40c60d2320e583ee11a2548aaef219d57061fc790b208803f7b0b66575cd5bc3ebdb82f91cad6c977b38475e0d65e5f928ffe8bef755cab9fced393d1d92c33c86cd9ae03f23dc542dc57fa12984097c7398ddb82383e8790f243afe4a866b9a6e079856a4eb369
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