Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard.
We have developed a very simple method, termed absorbance summation (AS), for comparing protein concentrations between samples in ELISA assays without a standard. This method sums the observed absorbance values from all dilutions to obtain one data point for each sample to be used for comparison. AS...
Saved in:
| _version_ | 1850230400057606144 |
|---|---|
| author | Holly Hartman Yuge Wang Harry W Schroeder Xiangqin Cui |
| author_facet | Holly Hartman Yuge Wang Harry W Schroeder Xiangqin Cui |
| author_sort | Holly Hartman |
| collection | DOAJ |
| description | We have developed a very simple method, termed absorbance summation (AS), for comparing protein concentrations between samples in ELISA assays without a standard. This method sums the observed absorbance values from all dilutions to obtain one data point for each sample to be used for comparison. AS is less computationally intensive than fitting sigmoidal curves, and it avoids the difficulty of parameter estimation for samples with absorbance values lying primarily at the lower tail of the curve. Our simulation studies showed that it performs much better than the sigmoidal curve fitting method and the conventional endpoint titer method. The power of this simple method is as high as the formal curve fitting followed by the estimation of area under the curve (AUC). |
| format | Article |
| id | doaj-art-dbbd708319cc4352be3ac6208bf697b6 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-dbbd708319cc4352be3ac6208bf697b62025-08-20T02:03:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019852810.1371/journal.pone.0198528Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard.Holly HartmanYuge WangHarry W SchroederXiangqin CuiWe have developed a very simple method, termed absorbance summation (AS), for comparing protein concentrations between samples in ELISA assays without a standard. This method sums the observed absorbance values from all dilutions to obtain one data point for each sample to be used for comparison. AS is less computationally intensive than fitting sigmoidal curves, and it avoids the difficulty of parameter estimation for samples with absorbance values lying primarily at the lower tail of the curve. Our simulation studies showed that it performs much better than the sigmoidal curve fitting method and the conventional endpoint titer method. The power of this simple method is as high as the formal curve fitting followed by the estimation of area under the curve (AUC).https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198528/1/pone.0198528.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T113251Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| spellingShingle | Holly Hartman Yuge Wang Harry W Schroeder Xiangqin Cui Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard. PLoS ONE |
| title | Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard. |
| title_full | Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard. |
| title_fullStr | Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard. |
| title_full_unstemmed | Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard. |
| title_short | Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard. |
| title_sort | absorbance summation a novel approach for analyzing high throughput elisa data in the absence of a standard |
| url | https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198528/1/pone.0198528.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T113251Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| work_keys_str_mv | AT hollyhartman absorbancesummationanovelapproachforanalyzinghighthroughputelisadataintheabsenceofastandard AT yugewang absorbancesummationanovelapproachforanalyzinghighthroughputelisadataintheabsenceofastandard AT harrywschroeder absorbancesummationanovelapproachforanalyzinghighthroughputelisadataintheabsenceofastandard AT xiangqincui absorbancesummationanovelapproachforanalyzinghighthroughputelisadataintheabsenceofastandard |