Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic Conditions
Ethanol and xylitol are valuable bioproducts synthesized by non-conventional yeasts from lignocellulosic sugars. However, their biosynthesis requires distinct cultivation conditions. This study evaluated the production of ethanol and xylitol by <i>Clavispora lusitaniae</i> using sacchari...
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2025-06-01
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| author | David Guzmán-Hernández Ana C. Ramos-Valdivia Héctor Mario Poggi-Varaldo Josefina Barrera-Cortés Eliseo Cristiani-Urbina Teresa Ponce-Noyola |
| author_facet | David Guzmán-Hernández Ana C. Ramos-Valdivia Héctor Mario Poggi-Varaldo Josefina Barrera-Cortés Eliseo Cristiani-Urbina Teresa Ponce-Noyola |
| author_sort | David Guzmán-Hernández |
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| description | Ethanol and xylitol are valuable bioproducts synthesized by non-conventional yeasts from lignocellulosic sugars. However, their biosynthesis requires distinct cultivation conditions. This study evaluated the production of ethanol and xylitol by <i>Clavispora lusitaniae</i> using saccharified sugarcane bagasse (SSCB) under three aeration conditions: microaerobic (C1), anaerobic (C2), and a combination of anaerobic followed by a microaerobic phase (C3). Ethanol production was maximum under anaerobic conditions (C2), followed by combined anaerobic–microaerobic conditions (C3). Meanwhile, xylitol production was most efficient under microaerobic conditions (C1). Notably, anaerobic conditions were ineffective for xylitol production. Enzyme activities of xylose reductase (XR) and xylitol dehydrogenase (XDH), key enzymes in xylose metabolism, were highest under microaerobic conditions with activities of 2.88 U/mg and 1.72 U/mg, respectively, after 48 h of culture. Gene expression analysis of <i>XYL</i>1 and <i>XYL</i>2 correlated with the corresponding enzyme activities (XR) and (XDH) with increased levels of 32.38 and 7.88 fold, respectively, compared to the control in C1. These findings suggest that <i>C. lusitaniae</i> co-produces ethanol efficiently under anaerobic conditions, while xylitol biosynthesis is optimized under microaerobic conditions when using xylose-rich saccharified lignocellulosic substrates. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-06-01 |
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| series | Fermentation |
| spelling | doaj-art-dba3357e195a471d88eb027769ed219c2025-08-20T03:27:22ZengMDPI AGFermentation2311-56372025-06-0111634410.3390/fermentation11060344Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic ConditionsDavid Guzmán-Hernández0Ana C. Ramos-Valdivia1Héctor Mario Poggi-Varaldo2Josefina Barrera-Cortés3Eliseo Cristiani-Urbina4Teresa Ponce-Noyola5Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Ciudad de México 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Ciudad de México 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Ciudad de México 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Ciudad de México 07360, MexicoDepartamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Ciudad de México 07738, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Ciudad de México 07360, MexicoEthanol and xylitol are valuable bioproducts synthesized by non-conventional yeasts from lignocellulosic sugars. However, their biosynthesis requires distinct cultivation conditions. This study evaluated the production of ethanol and xylitol by <i>Clavispora lusitaniae</i> using saccharified sugarcane bagasse (SSCB) under three aeration conditions: microaerobic (C1), anaerobic (C2), and a combination of anaerobic followed by a microaerobic phase (C3). Ethanol production was maximum under anaerobic conditions (C2), followed by combined anaerobic–microaerobic conditions (C3). Meanwhile, xylitol production was most efficient under microaerobic conditions (C1). Notably, anaerobic conditions were ineffective for xylitol production. Enzyme activities of xylose reductase (XR) and xylitol dehydrogenase (XDH), key enzymes in xylose metabolism, were highest under microaerobic conditions with activities of 2.88 U/mg and 1.72 U/mg, respectively, after 48 h of culture. Gene expression analysis of <i>XYL</i>1 and <i>XYL</i>2 correlated with the corresponding enzyme activities (XR) and (XDH) with increased levels of 32.38 and 7.88 fold, respectively, compared to the control in C1. These findings suggest that <i>C. lusitaniae</i> co-produces ethanol efficiently under anaerobic conditions, while xylitol biosynthesis is optimized under microaerobic conditions when using xylose-rich saccharified lignocellulosic substrates.https://www.mdpi.com/2311-5637/11/6/344<i>Clavispora lusitaniae</i>xylitolethanolxylose reductasexylitol dehydrogenase |
| spellingShingle | David Guzmán-Hernández Ana C. Ramos-Valdivia Héctor Mario Poggi-Varaldo Josefina Barrera-Cortés Eliseo Cristiani-Urbina Teresa Ponce-Noyola Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic Conditions Fermentation <i>Clavispora lusitaniae</i> xylitol ethanol xylose reductase xylitol dehydrogenase |
| title | Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic Conditions |
| title_full | Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic Conditions |
| title_fullStr | Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic Conditions |
| title_full_unstemmed | Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic Conditions |
| title_short | Ethanol and Xylitol Co-Production by <i>Clavispora lusitaniae</i> Growing on Saccharified Sugar Cane Bagasse in Anaerobic/Microaerobic Conditions |
| title_sort | ethanol and xylitol co production by i clavispora lusitaniae i growing on saccharified sugar cane bagasse in anaerobic microaerobic conditions |
| topic | <i>Clavispora lusitaniae</i> xylitol ethanol xylose reductase xylitol dehydrogenase |
| url | https://www.mdpi.com/2311-5637/11/6/344 |
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