Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study
[Objective:] To study the effects of a Chinese traditional medicine icariin (ICA) on the cell activity and osteogenic differentiation of preosteoblasts in mice calvarium (MC3T3-E1). [Methods:] The optimal intervention concentration of ICA was screened and MC3T3-E1 cells were divided into blank contr...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-08-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.003 |
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| author | QIAN Hongjing LIU Yulian ZHANG Yunpeng SHI Yiming HUANG Guanghui ZHOU Yeran LUO Guangming |
| author_facet | QIAN Hongjing LIU Yulian ZHANG Yunpeng SHI Yiming HUANG Guanghui ZHOU Yeran LUO Guangming |
| author_sort | QIAN Hongjing |
| collection | DOAJ |
| description | [Objective:] To study the effects of a Chinese traditional medicine icariin (ICA) on the cell activity and osteogenic differentiation of preosteoblasts in mice calvarium (MC3T3-E1). [Methods:] The optimal intervention concentration of ICA was screened and MC3T3-E1 cells were divided into blank control group and experimental group with different concentrations of ICA. MC3T3-E1 cells were treated with ICA complete medium containing 10-9, 10-8, 10-7, 10-6 and 10-5 mol/L. On days 2, 4 and 6, the proliferation of MC3T3-E1 cells was detected by CCK8 kit, and the ALP activity was detected by alkaline phosphatase (ALP) kit on days 3, 6 and 9; MC3T3-E1 cells were treated with 10-7 mol/L ICA complete medium for 21 days. Alizarin red (AR) staining was used to determine the formation of calcium nodules. [Results:] Compared with the blank control group, the drug treatment group significantly inhibited the proliferation of MC3T3-E1 cells, but promoted the ALP activity of MC3T3-E1 cells; however, different concentrations of ICA had no significant effect on the formation of calcium nodules in cultured cells. [Conclusion:] Under our experimental conditions, ICA may inhibit the proliferation of MC3T3-E1 cells and promote osteogenic differentiation of MC3T3-E1 cells. |
| format | Article |
| id | doaj-art-dba2bc6abbd54c08bb91590059cb5f8f |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2023-08-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-dba2bc6abbd54c08bb91590059cb5f8f2025-08-25T06:10:14ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792023-08-0133421922310.12439/kqhm.1005-4979.2023.04.003Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental studyQIAN Hongjing0LIU Yulian1ZHANG Yunpeng2SHI Yiming3HUANG Guanghui4ZHOU Yeran5LUO Guangming6School and Hospital of Stomatology, Kunming Medical University, Yunnan Key Laboratory of Stomatology, Kunming 650106, Yunnan Province, ChinaSchool and Hospital of Stomatology, Kunming Medical University, Yunnan Key Laboratory of Stomatology, Kunming 650106, Yunnan Province, ChinaSchool and Hospital of Stomatology, Kunming Medical University, Yunnan Key Laboratory of Stomatology, Kunming 650106, Yunnan Province, ChinaSchool and Hospital of Stomatology, Kunming Medical University, Yunnan Key Laboratory of Stomatology, Kunming 650106, Yunnan Province, ChinaSchool and Hospital of Stomatology, Kunming Medical University, Yunnan Key Laboratory of Stomatology, Kunming 650106, Yunnan Province, ChinaSchool and Hospital of Stomatology, Kunming Medical University, Yunnan Key Laboratory of Stomatology, Kunming 650106, Yunnan Province, ChinaSchool and Hospital of Stomatology, Kunming Medical University, Yunnan Key Laboratory of Stomatology, Kunming 650106, Yunnan Province, China[Objective:] To study the effects of a Chinese traditional medicine icariin (ICA) on the cell activity and osteogenic differentiation of preosteoblasts in mice calvarium (MC3T3-E1). [Methods:] The optimal intervention concentration of ICA was screened and MC3T3-E1 cells were divided into blank control group and experimental group with different concentrations of ICA. MC3T3-E1 cells were treated with ICA complete medium containing 10-9, 10-8, 10-7, 10-6 and 10-5 mol/L. On days 2, 4 and 6, the proliferation of MC3T3-E1 cells was detected by CCK8 kit, and the ALP activity was detected by alkaline phosphatase (ALP) kit on days 3, 6 and 9; MC3T3-E1 cells were treated with 10-7 mol/L ICA complete medium for 21 days. Alizarin red (AR) staining was used to determine the formation of calcium nodules. [Results:] Compared with the blank control group, the drug treatment group significantly inhibited the proliferation of MC3T3-E1 cells, but promoted the ALP activity of MC3T3-E1 cells; however, different concentrations of ICA had no significant effect on the formation of calcium nodules in cultured cells. [Conclusion:] Under our experimental conditions, ICA may inhibit the proliferation of MC3T3-E1 cells and promote osteogenic differentiation of MC3T3-E1 cells.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.003icariinmc3t3-e1 cellsproliferationosteogenic differentiation |
| spellingShingle | QIAN Hongjing LIU Yulian ZHANG Yunpeng SHI Yiming HUANG Guanghui ZHOU Yeran LUO Guangming Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study Kouqiang hemian waike zazhi icariin mc3t3-e1 cells proliferation osteogenic differentiation |
| title | Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study |
| title_full | Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study |
| title_fullStr | Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study |
| title_full_unstemmed | Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study |
| title_short | Icariin on the proliferation and osteogenic differentiation of MC3T3-E1: An experimental study |
| title_sort | icariin on the proliferation and osteogenic differentiation of mc3t3 e1 an experimental study |
| topic | icariin mc3t3-e1 cells proliferation osteogenic differentiation |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.003 |
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