Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?

This study investigated whether the suspension method (tenderstretch, TS or Achilles tendon, AT) can improve the quality of horsemeat by analyzing longissimus dorsi (LD) and semitendinosus (ST) muscles. A total of 25 horse carcasses were considered experimental units and split longitudinally, with o...

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Main Authors: Ana Kaić, Barbara Luštrek, Silvester Žgur, Klemen Potočnik
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/14/23/3540
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author Ana Kaić
Barbara Luštrek
Silvester Žgur
Klemen Potočnik
author_facet Ana Kaić
Barbara Luštrek
Silvester Žgur
Klemen Potočnik
author_sort Ana Kaić
collection DOAJ
description This study investigated whether the suspension method (tenderstretch, TS or Achilles tendon, AT) can improve the quality of horsemeat by analyzing longissimus dorsi (LD) and semitendinosus (ST) muscles. A total of 25 horse carcasses were considered experimental units and split longitudinally, with one half suspended using the TS method and the other half using the AT method, which enabled a direct comparison within the carcass. After 7 days of aging under commercial processing conditions, the LD and ST muscles were analyzed for pH, color (L*, a*, b*), water-holding capacity (drip loss, thawing loss, cooking loss), tenderness (Warner–Bratzler shear force), and sarcomere length. Statistical analysis was performed using the MIXED procedure in SAS, with Bonferroni correction applied for post hoc comparisons. Significant differences were found between the muscles: LD had higher tenderness (39.28 N vs. 49.77 N, <i>p</i> = 0.0011), lower cooking loss (23.56% vs. 27.04%, <i>p</i> = 0.0002), and higher thawing loss (12.38% vs. 9.72%, <i>p</i> = 0.0021) compared to ST muscle, which had a lighter color (L* = 41.90 vs. 37.73, <i>p</i> < 0.0001) and longer sarcomeres (2.22 μm vs. 1.74 μm, <i>p</i> < 0.0001). While the TS suspension method significantly increased sarcomere length (2.05 μm vs. 1.92 μm, <i>p</i> = 0.0020), it did not lead to significant improvements in other quality attributes such as pH, water-holding capacity, or tenderness. The results indicate that although the TS method affects muscle structure by elongating sarcomeres (with an average difference of 0.13 μm), it does not significantly improve the overall quality of the horsemeat compared to the AT method after 7 days of aging. A combination of factors beyond suspension methods, such as optimizing aging periods or considering additional processing techniques, may therefore be required to improve horsemeat quality. This study provides insights into the specific attributes of LD and ST muscles and their response to suspension methods and contributes to a better understanding of optimizing horsemeat quality for commercial purposes.
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spelling doaj-art-dba1459f5ad642909a1a15f2cec7e2802025-08-20T02:38:35ZengMDPI AGAnimals2076-26152024-12-011423354010.3390/ani14233540Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?Ana Kaić0Barbara Luštrek1Silvester Žgur2Klemen Potočnik3Department of Animal Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, CroatiaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaThis study investigated whether the suspension method (tenderstretch, TS or Achilles tendon, AT) can improve the quality of horsemeat by analyzing longissimus dorsi (LD) and semitendinosus (ST) muscles. A total of 25 horse carcasses were considered experimental units and split longitudinally, with one half suspended using the TS method and the other half using the AT method, which enabled a direct comparison within the carcass. After 7 days of aging under commercial processing conditions, the LD and ST muscles were analyzed for pH, color (L*, a*, b*), water-holding capacity (drip loss, thawing loss, cooking loss), tenderness (Warner–Bratzler shear force), and sarcomere length. Statistical analysis was performed using the MIXED procedure in SAS, with Bonferroni correction applied for post hoc comparisons. Significant differences were found between the muscles: LD had higher tenderness (39.28 N vs. 49.77 N, <i>p</i> = 0.0011), lower cooking loss (23.56% vs. 27.04%, <i>p</i> = 0.0002), and higher thawing loss (12.38% vs. 9.72%, <i>p</i> = 0.0021) compared to ST muscle, which had a lighter color (L* = 41.90 vs. 37.73, <i>p</i> < 0.0001) and longer sarcomeres (2.22 μm vs. 1.74 μm, <i>p</i> < 0.0001). While the TS suspension method significantly increased sarcomere length (2.05 μm vs. 1.92 μm, <i>p</i> = 0.0020), it did not lead to significant improvements in other quality attributes such as pH, water-holding capacity, or tenderness. The results indicate that although the TS method affects muscle structure by elongating sarcomeres (with an average difference of 0.13 μm), it does not significantly improve the overall quality of the horsemeat compared to the AT method after 7 days of aging. A combination of factors beyond suspension methods, such as optimizing aging periods or considering additional processing techniques, may therefore be required to improve horsemeat quality. This study provides insights into the specific attributes of LD and ST muscles and their response to suspension methods and contributes to a better understanding of optimizing horsemeat quality for commercial purposes.https://www.mdpi.com/2076-2615/14/23/3540hanging methodhorsemeat qualitymuscles
spellingShingle Ana Kaić
Barbara Luštrek
Silvester Žgur
Klemen Potočnik
Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?
Animals
hanging method
horse
meat quality
muscles
title Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?
title_full Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?
title_fullStr Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?
title_full_unstemmed Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?
title_short Can the Suspension Method (Tenderstretch vs. Achilles Tendon) Enhance Horsemeat Quality?
title_sort can the suspension method tenderstretch vs achilles tendon enhance horsemeat quality
topic hanging method
horse
meat quality
muscles
url https://www.mdpi.com/2076-2615/14/23/3540
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AT klemenpotocnik canthesuspensionmethodtenderstretchvsachillestendonenhancehorsemeatquality