Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioides
Anthracnose is one of the most destructive diseases and is widely prevalent in world. Due to the limited understanding of the resistance mechanisms, the insufficient of tea anthracnose control cause yield and quality losses. This study established a lesion coefficient calculation model using ImageJ...
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Elsevier
2025-06-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X25001307 |
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| author | Jing-Jing Ye Han-Qing Gao Yun-Long Shi Xin-Yi Lin Yue-Rong Liang Jian-Liang Lu Jian-Hui Ye Xin-Qiang Zheng |
| author_facet | Jing-Jing Ye Han-Qing Gao Yun-Long Shi Xin-Yi Lin Yue-Rong Liang Jian-Liang Lu Jian-Hui Ye Xin-Qiang Zheng |
| author_sort | Jing-Jing Ye |
| collection | DOAJ |
| description | Anthracnose is one of the most destructive diseases and is widely prevalent in world. Due to the limited understanding of the resistance mechanisms, the insufficient of tea anthracnose control cause yield and quality losses. This study established a lesion coefficient calculation model using ImageJ software and identified a resistant variety (Camellia. sinensis var. laochashan) and a susceptible variety (Camellia. sinensis var. zhenong402). Using time-resolved dual RNA-seq technology, the differential expression genes (DEGs) of two tea varieties and Colletotrichum gloeosporioides during the infection period (0 h, 12 h, 30 h, 72 h, and 120 h) were figured. Genes associated with appressorium formation, enzymes, mycotoxins, and common fungal extracellular membrane (CFEM) effector proteins may be involved in the infection process of anthracnose. Conversely, the DEGs related to the cell wall components, phytohormones, signal transduction, transcription factors, secondary metabolites, and pathogenesis-related proteins (PRs) may function in the disease resistance of tea plants. Weighted Gene Co-expression Network Analysis (WGCNA) revealed modules of positively/negatively correlated genes that may contribute to defense in resistant variety. Parallel Reaction Monitoring (PRM) quantification of representative PRs (PR1, CHIT, TLP, and PAL) clarified their potential roles in the defense response of tea plants. The findings of this study provide foundational insights into tea plant anthracnose resistance mechanisms and innovative disease management approaches. |
| format | Article |
| id | doaj-art-db9736d37c384122a87e6b155f1c45bf |
| institution | DOAJ |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-db9736d37c384122a87e6b155f1c45bf2025-08-20T02:56:55ZengElsevierPlant Stress2667-064X2025-06-011610086510.1016/j.stress.2025.100865Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioidesJing-Jing Ye0Han-Qing Gao1Yun-Long Shi2Xin-Yi Lin3Yue-Rong Liang4Jian-Liang Lu5Jian-Hui Ye6Xin-Qiang Zheng7Tea Research Institute, Zhejiang University, Zhejiang 310058, ChinaTea Research Institute, Zhejiang University, Zhejiang 310058, ChinaTea Research Institute, Zhejiang University, Zhejiang 310058, China; Huzhou High-Level Talent Service Center, Zhejiang 313000, ChinaTea Research Institute, Zhejiang University, Zhejiang 310058, ChinaTea Research Institute, Zhejiang University, Zhejiang 310058, ChinaTea Research Institute, Zhejiang University, Zhejiang 310058, ChinaTea Research Institute, Zhejiang University, Zhejiang 310058, ChinaTea Research Institute, Zhejiang University, Zhejiang 310058, China; Corresponding author.Anthracnose is one of the most destructive diseases and is widely prevalent in world. Due to the limited understanding of the resistance mechanisms, the insufficient of tea anthracnose control cause yield and quality losses. This study established a lesion coefficient calculation model using ImageJ software and identified a resistant variety (Camellia. sinensis var. laochashan) and a susceptible variety (Camellia. sinensis var. zhenong402). Using time-resolved dual RNA-seq technology, the differential expression genes (DEGs) of two tea varieties and Colletotrichum gloeosporioides during the infection period (0 h, 12 h, 30 h, 72 h, and 120 h) were figured. Genes associated with appressorium formation, enzymes, mycotoxins, and common fungal extracellular membrane (CFEM) effector proteins may be involved in the infection process of anthracnose. Conversely, the DEGs related to the cell wall components, phytohormones, signal transduction, transcription factors, secondary metabolites, and pathogenesis-related proteins (PRs) may function in the disease resistance of tea plants. Weighted Gene Co-expression Network Analysis (WGCNA) revealed modules of positively/negatively correlated genes that may contribute to defense in resistant variety. Parallel Reaction Monitoring (PRM) quantification of representative PRs (PR1, CHIT, TLP, and PAL) clarified their potential roles in the defense response of tea plants. The findings of this study provide foundational insights into tea plant anthracnose resistance mechanisms and innovative disease management approaches.http://www.sciencedirect.com/science/article/pii/S2667064X25001307TeaAnthracnoseResistanceTime-resolved dual transcriptomicsPRs |
| spellingShingle | Jing-Jing Ye Han-Qing Gao Yun-Long Shi Xin-Yi Lin Yue-Rong Liang Jian-Liang Lu Jian-Hui Ye Xin-Qiang Zheng Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioides Plant Stress Tea Anthracnose Resistance Time-resolved dual transcriptomics PRs |
| title | Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioides |
| title_full | Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioides |
| title_fullStr | Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioides |
| title_full_unstemmed | Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioides |
| title_short | Time-resolved dual transcriptome profiling of interactions between tea plants (Camellia sinensis (L.) O. Kuntze) and its anthracnose pathogen Colletotrichum gloeosporioides |
| title_sort | time resolved dual transcriptome profiling of interactions between tea plants camellia sinensis l o kuntze and its anthracnose pathogen colletotrichum gloeosporioides |
| topic | Tea Anthracnose Resistance Time-resolved dual transcriptomics PRs |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X25001307 |
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