Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens
IntroductionChinese medicinal herbs play important roles in anti-inflammatory, antioxidant, and antibacterial activities. However, the effects of Chinese herb ultrafine powder (CHUP) on laying hens still need to be elucidated. Therefore, this study aimed to evaluate the effects of dietary CHUP suppl...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| author | Jue Gui Jue Gui Md Abul Kalam Azad Wenchao Lin Wenchao Lin Chengwen Meng Chengwen Meng Xin Hu Yadong Cui Wei Lan Jianhua He Xiangfeng Kong Xiangfeng Kong Xiangfeng Kong |
| author_facet | Jue Gui Jue Gui Md Abul Kalam Azad Wenchao Lin Wenchao Lin Chengwen Meng Chengwen Meng Xin Hu Yadong Cui Wei Lan Jianhua He Xiangfeng Kong Xiangfeng Kong Xiangfeng Kong |
| author_sort | Jue Gui |
| collection | DOAJ |
| description | IntroductionChinese medicinal herbs play important roles in anti-inflammatory, antioxidant, and antibacterial activities. However, the effects of Chinese herb ultrafine powder (CHUP) on laying hens still need to be elucidated. Therefore, this study aimed to evaluate the effects of dietary CHUP supplementation on jejunal morphology, physical barrier function, and microbiota in laying hens.MethodsA total of 576 Xinyang black-feather laying hens (300 days old) were randomly assigned into eight groups, with eight replicates per group and nine hens per replicate. The hens were fed a basal diet (control group) and a basal diet supplemented with 0.5% Leonuri herba (LH group), 0.25% Ligustri lucidi fructus (LF group), 0.25% Taraxaci herba (TH group), 0.5% LH + 0.25% LF (LH-LF group), 0.5% LH + 0.25% TH (LH-TH group), 0.25% LF + 0.25% TH (LF-TH group), and 0.5% LH + 0.25% LF + 0.25% TH (LH-LF-TH group), respectively, for 120 days.ResultsThe results showed that dietary LH-LF and LH-LF-TH supplementation increased (p < 0.05) the jejunal villus height to crypt depth ratio of laying hens. Dietary LF-TH supplementation up-regulated jejunal claudin-5 expression, while LH supplementation up-regulated jejunal claudin-1 expression and increased the jejunal abundances of potentially beneficial bacteria related to short-chain fatty acids and bacteriocins production, such as Blautia, Carnobacterium, Clostridiales, and Erysipelotrichales (p < 0.05). In addition, dietary LH supplementation enriched (p < 0.05) the tetracycline biosynthesis, butirosin/neomycin biosynthesis, and D-arginine/D-ornithine metabolism, whereas steroid biosynthesis and limonene/pinene degradation were enriched (p < 0.05) in the LH-LF and LH-LF-TH groups. Moreover, Spearman’s correlation analysis revealed the potential correlation between the abundance of the jejunal microbiota and jejunal morphology and the physical barrier function of laying hens.DiscussionCollectively, these findings suggest that dietary CHUP supplementation could enhance the beneficial bacteria abundance, physical barrier function, and metabolic function associated with short-chain fatty acids and bacteriocins production. Moreover, combined supplementation of dietary CHUP showed better effects than the sole CHUP supplementation. |
| format | Article |
| id | doaj-art-db8f42fc3ec1401096f15feba793b2b6 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2023-05-01 |
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| spelling | doaj-art-db8f42fc3ec1401096f15feba793b2b62025-08-20T03:09:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11858061185806Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hensJue Gui0Jue Gui1Md Abul Kalam Azad2Wenchao Lin3Wenchao Lin4Chengwen Meng5Chengwen Meng6Xin Hu7Yadong Cui8Wei Lan9Jianhua He10Xiangfeng Kong11Xiangfeng Kong12Xiangfeng Kong13Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaSchool of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaSchool of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, ChinaSchool of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, ChinaSchool of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, ChinaSchool of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaSchool of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaIntroductionChinese medicinal herbs play important roles in anti-inflammatory, antioxidant, and antibacterial activities. However, the effects of Chinese herb ultrafine powder (CHUP) on laying hens still need to be elucidated. Therefore, this study aimed to evaluate the effects of dietary CHUP supplementation on jejunal morphology, physical barrier function, and microbiota in laying hens.MethodsA total of 576 Xinyang black-feather laying hens (300 days old) were randomly assigned into eight groups, with eight replicates per group and nine hens per replicate. The hens were fed a basal diet (control group) and a basal diet supplemented with 0.5% Leonuri herba (LH group), 0.25% Ligustri lucidi fructus (LF group), 0.25% Taraxaci herba (TH group), 0.5% LH + 0.25% LF (LH-LF group), 0.5% LH + 0.25% TH (LH-TH group), 0.25% LF + 0.25% TH (LF-TH group), and 0.5% LH + 0.25% LF + 0.25% TH (LH-LF-TH group), respectively, for 120 days.ResultsThe results showed that dietary LH-LF and LH-LF-TH supplementation increased (p < 0.05) the jejunal villus height to crypt depth ratio of laying hens. Dietary LF-TH supplementation up-regulated jejunal claudin-5 expression, while LH supplementation up-regulated jejunal claudin-1 expression and increased the jejunal abundances of potentially beneficial bacteria related to short-chain fatty acids and bacteriocins production, such as Blautia, Carnobacterium, Clostridiales, and Erysipelotrichales (p < 0.05). In addition, dietary LH supplementation enriched (p < 0.05) the tetracycline biosynthesis, butirosin/neomycin biosynthesis, and D-arginine/D-ornithine metabolism, whereas steroid biosynthesis and limonene/pinene degradation were enriched (p < 0.05) in the LH-LF and LH-LF-TH groups. Moreover, Spearman’s correlation analysis revealed the potential correlation between the abundance of the jejunal microbiota and jejunal morphology and the physical barrier function of laying hens.DiscussionCollectively, these findings suggest that dietary CHUP supplementation could enhance the beneficial bacteria abundance, physical barrier function, and metabolic function associated with short-chain fatty acids and bacteriocins production. Moreover, combined supplementation of dietary CHUP showed better effects than the sole CHUP supplementation.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1185806/fullChinese herbintestinal barrier functionmicrobiotaultrafine powderXinyang black-feather hens |
| spellingShingle | Jue Gui Jue Gui Md Abul Kalam Azad Wenchao Lin Wenchao Lin Chengwen Meng Chengwen Meng Xin Hu Yadong Cui Wei Lan Jianhua He Xiangfeng Kong Xiangfeng Kong Xiangfeng Kong Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens Frontiers in Microbiology Chinese herb intestinal barrier function microbiota ultrafine powder Xinyang black-feather hens |
| title | Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens |
| title_full | Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens |
| title_fullStr | Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens |
| title_full_unstemmed | Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens |
| title_short | Dietary supplementation with Chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens |
| title_sort | dietary supplementation with chinese herb ultrafine powder improves intestinal morphology and physical barrier function by altering jejunal microbiota in laying hens |
| topic | Chinese herb intestinal barrier function microbiota ultrafine powder Xinyang black-feather hens |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1185806/full |
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