The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells
The aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor β-1 (TGF β1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with γ-interferon. The effects of IL-10 alone,...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359891315 |
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| author | M. J. Zimmerman G. R. Radford-Smith D. P. Jewell |
| author_facet | M. J. Zimmerman G. R. Radford-Smith D. P. Jewell |
| author_sort | M. J. Zimmerman |
| collection | DOAJ |
| description | The aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor β-1 (TGF β1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with γ-interferon. The effects of IL-10 alone, TGF β1 alone, and IL-10 and TGF β1 in combination were studied. HLA-DR expression was assessed using flow cytometric analysis. γ-Interferon induced HLA-DR expression in a dose-dependent fashion. In the absence of γ-interferon, neither IL-10 nor TGF β1 induced HLA-DR expression. In isolation, neither IL10 nor TGF β1 downregulated HLA-DR expression. When IL-10 and TGF β1 were added in combination, small (6-30%) statistically significant reductions in HLA-DR expression were seen. The biological significance is unclear. |
| format | Article |
| id | doaj-art-db7cd60af0c54c57a536f24a2d766b23 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-db7cd60af0c54c57a536f24a2d766b232025-02-03T01:03:23ZengWileyMediators of Inflammation0962-93511466-18611998-01-017171110.1080/09629359891315The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cellsM. J. Zimmerman0G. R. Radford-Smith1D. P. Jewell2Gastroenterology Department, Royal Perth Hospital, Wellington Street , Perth 6001, Western Australia, AustraliaDepartment of Gastroenterology, Royal Brisbane Hospital, Herston Road, Brisbane 4029, Queensland, AustraliaGastroenterology Unit, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, UKThe aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor β-1 (TGF β1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with γ-interferon. The effects of IL-10 alone, TGF β1 alone, and IL-10 and TGF β1 in combination were studied. HLA-DR expression was assessed using flow cytometric analysis. γ-Interferon induced HLA-DR expression in a dose-dependent fashion. In the absence of γ-interferon, neither IL-10 nor TGF β1 induced HLA-DR expression. In isolation, neither IL10 nor TGF β1 downregulated HLA-DR expression. When IL-10 and TGF β1 were added in combination, small (6-30%) statistically significant reductions in HLA-DR expression were seen. The biological significance is unclear.http://dx.doi.org/10.1080/09629359891315InterLeukin-10Transforming Growth Factor β1γ-InterferonHLA-DRHT29 Cell Culture. |
| spellingShingle | M. J. Zimmerman G. R. Radford-Smith D. P. Jewell The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells Mediators of Inflammation InterLeukin-10 Transforming Growth Factor β1 γ-Interferon HLA-DR HT29 Cell Culture. |
| title | The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells |
| title_full | The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells |
| title_fullStr | The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells |
| title_full_unstemmed | The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells |
| title_short | The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells |
| title_sort | effect of interleukin 10 and transforming growth factor β 1 on hla dr expression in colonic epithelial cells |
| topic | InterLeukin-10 Transforming Growth Factor β1 γ-Interferon HLA-DR HT29 Cell Culture. |
| url | http://dx.doi.org/10.1080/09629359891315 |
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