Lactoferrin Induces Osteoblast Growth through IGF-1R
Objectives. To investigate the role of the IGF-1R by which lactoferrin induces osteoblast growth. Methods. Osteoblast received 5 d lactoferrin intervention at a concentration of 0.1, 1, 10, 100, and 1000 μg/mL, and the IGF-1 and IGF-1R were detected using RT-PCR and western blot. The osteoblast into...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/282806 |
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| author | Jian-Ming Hou En-Yu Chen Fan Lin Qing-Ming Lin Ying Xue Xu-Hua Lan Man Wu |
| author_facet | Jian-Ming Hou En-Yu Chen Fan Lin Qing-Ming Lin Ying Xue Xu-Hua Lan Man Wu |
| author_sort | Jian-Ming Hou |
| collection | DOAJ |
| description | Objectives. To investigate the role of the IGF-1R by which lactoferrin induces osteoblast growth. Methods. Osteoblast received 5 d lactoferrin intervention at a concentration of 0.1, 1, 10, 100, and 1000 μg/mL, and the IGF-1 and IGF-1R were detected using RT-PCR and western blot. The osteoblast into the control, 100 μg/mL lactoferrin, Neo-scramble (NS, empty vector), NS + 100 μg/mL lactoferrin, shIGF-1R and shIGF-1R + 100 μg/mL lactoferrin group. We test the apoptosis and proliferation and the level of PI3K and RAS in osteoblasts after 5 d intervention. Results. (1) 1, 10, 100, and 1000 μg/mL lactoferrin induced the expression of IGF-1 mRNA and protein. 10 μg/mL and 100 μg/mL lactoferrin induced the expression of IGF-1R mRNA and protein. (2) Lactoferrin (100 μg/mL) induced osteoblast proliferation while inhibiting apoptosis. Osteoblasts with silenced IGF-1R exhibited decreased proliferation but increased apoptosis. MMT staining and flow cytometry both indicated that there was no significant difference between the shIGF-1R group and the shIGF-1R + 100 μg/mL lactoferrin group. (3) Lactoferrin (100 μg/mL) induced PI3K and RAS phosphorylation and silence of IGF-1R resulted in decreased p-PI3K and p-RAS expression. Lactoferrin-treated shIGF-1R cells showed significantly higher level of p-PI3K and p-RAS when compared with shIGF-1R. Conclusion. Lactoferrin induced IGF-1/IGF-1R in a concentration-dependent manner. Lactoferrin promoted osteoblast proliferation while inhibiting apoptosis through IGF-1R. Lactoferrin activated PI3K and RAS phosphorylation via an IGF-1R independent pathway. |
| format | Article |
| id | doaj-art-db7792548b6c4df391f69f4a011cf3d9 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-db7792548b6c4df391f69f4a011cf3d92025-02-03T05:45:05ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/282806282806Lactoferrin Induces Osteoblast Growth through IGF-1RJian-Ming Hou0En-Yu Chen1Fan Lin2Qing-Ming Lin3Ying Xue4Xu-Hua Lan5Man Wu6Endocrinology Department, Fujian Provincial Hospital, No. 134 Dong Jie Road, Fuzhou, Fujian 350001, ChinaEndocrinology Department, Fujian Provincial Hospital, No. 134 Dong Jie Road, Fuzhou, Fujian 350001, ChinaEndocrinology Department, Fujian Provincial Hospital, No. 134 Dong Jie Road, Fuzhou, Fujian 350001, ChinaProvincial Clinical Medical College of Fujian Medical University, No. 134 Dong Jie Road, Fuzhou, Fujian 350001, ChinaProvincial Clinical Medical College of Fujian Medical University, No. 134 Dong Jie Road, Fuzhou, Fujian 350001, ChinaProvincial Clinical Medical College of Fujian Medical University, No. 134 Dong Jie Road, Fuzhou, Fujian 350001, ChinaProvincial Clinical Medical College of Fujian Medical University, No. 134 Dong Jie Road, Fuzhou, Fujian 350001, ChinaObjectives. To investigate the role of the IGF-1R by which lactoferrin induces osteoblast growth. Methods. Osteoblast received 5 d lactoferrin intervention at a concentration of 0.1, 1, 10, 100, and 1000 μg/mL, and the IGF-1 and IGF-1R were detected using RT-PCR and western blot. The osteoblast into the control, 100 μg/mL lactoferrin, Neo-scramble (NS, empty vector), NS + 100 μg/mL lactoferrin, shIGF-1R and shIGF-1R + 100 μg/mL lactoferrin group. We test the apoptosis and proliferation and the level of PI3K and RAS in osteoblasts after 5 d intervention. Results. (1) 1, 10, 100, and 1000 μg/mL lactoferrin induced the expression of IGF-1 mRNA and protein. 10 μg/mL and 100 μg/mL lactoferrin induced the expression of IGF-1R mRNA and protein. (2) Lactoferrin (100 μg/mL) induced osteoblast proliferation while inhibiting apoptosis. Osteoblasts with silenced IGF-1R exhibited decreased proliferation but increased apoptosis. MMT staining and flow cytometry both indicated that there was no significant difference between the shIGF-1R group and the shIGF-1R + 100 μg/mL lactoferrin group. (3) Lactoferrin (100 μg/mL) induced PI3K and RAS phosphorylation and silence of IGF-1R resulted in decreased p-PI3K and p-RAS expression. Lactoferrin-treated shIGF-1R cells showed significantly higher level of p-PI3K and p-RAS when compared with shIGF-1R. Conclusion. Lactoferrin induced IGF-1/IGF-1R in a concentration-dependent manner. Lactoferrin promoted osteoblast proliferation while inhibiting apoptosis through IGF-1R. Lactoferrin activated PI3K and RAS phosphorylation via an IGF-1R independent pathway.http://dx.doi.org/10.1155/2015/282806 |
| spellingShingle | Jian-Ming Hou En-Yu Chen Fan Lin Qing-Ming Lin Ying Xue Xu-Hua Lan Man Wu Lactoferrin Induces Osteoblast Growth through IGF-1R International Journal of Endocrinology |
| title | Lactoferrin Induces Osteoblast Growth through IGF-1R |
| title_full | Lactoferrin Induces Osteoblast Growth through IGF-1R |
| title_fullStr | Lactoferrin Induces Osteoblast Growth through IGF-1R |
| title_full_unstemmed | Lactoferrin Induces Osteoblast Growth through IGF-1R |
| title_short | Lactoferrin Induces Osteoblast Growth through IGF-1R |
| title_sort | lactoferrin induces osteoblast growth through igf 1r |
| url | http://dx.doi.org/10.1155/2015/282806 |
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