Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty

Percutaneous vertebroplasty (PVP) is widely used in the treatment of painful osteoporotic vertebral compression fractures with the injection of PMMA cement, and the controversy for PMMA damage to the osteoporotic bone tissue and to affect the fractures repairing never stops. 72 old female rabbits, e...

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Main Authors: ZhenMing Hu, Gang Zhao, LiJun Wang, Bo Pu, Jie Hao, HanChang Lao, XiaoJun Zhang, Qiang Gan, Wei Jiang
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2013/109784
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author ZhenMing Hu
Gang Zhao
LiJun Wang
Bo Pu
Jie Hao
HanChang Lao
XiaoJun Zhang
Qiang Gan
Wei Jiang
author_facet ZhenMing Hu
Gang Zhao
LiJun Wang
Bo Pu
Jie Hao
HanChang Lao
XiaoJun Zhang
Qiang Gan
Wei Jiang
author_sort ZhenMing Hu
collection DOAJ
description Percutaneous vertebroplasty (PVP) is widely used in the treatment of painful osteoporotic vertebral compression fractures with the injection of PMMA cement, and the controversy for PMMA damage to the osteoporotic bone tissue and to affect the fractures repairing never stops. 72 old female rabbits, each age 3.0~3.5 y, rabbits were assigned randomly to two groups of thirty-six each; PMMA cement were injected into vertebral body in rabbits via mimic PVP, sacrificed at 1 h, 24 h, 3 d, 7 d, 4 w, and 12 w. The expression VEGF and collagen type I, the tissue response, and repair reaction in the interface between PMMA and bone tissue were observed dynamically with RT-PCR and western blot technique; the osteocalcin expression were studied by immunohistochemistry. Compared with the control group, the expression of collagen I increased at 1 hour and was higher from 24 h to 3 d. From 4 weeks to 12 weeks after injection of PMMA. The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4–12 weeks. The osteocalcin expression continued to increase during 4 to 12 week. PMMA would not cause local bone permanent necrosis, and interface injury repairing cycle could be prolonged in a vertebroplasty.
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spelling doaj-art-db701c0670c3458e8a2f308cea5b7c6c2025-08-20T03:24:20ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/109784109784Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous VertebroplastyZhenMing Hu0Gang Zhao1LiJun Wang2Bo Pu3Jie Hao4HanChang Lao5XiaoJun Zhang6Qiang Gan7Wei Jiang8Department of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Orthopaedic Surgery, The Second Affiliated Hospital of Kunming Medical College, 1 Ma Yuan, Kunming, Yunnan 650101, ChinaDepartment of Orthopaedic Surgery, Dazu District Hospital of Chongqing, 138 West Longgang Road, Dazu District, Chongqing 402360, ChinaDepartment of Orthopaedic Surgery, The Second Affiliated Hospital of Kunming Medical College, 1 Ma Yuan, Kunming, Yunnan 650101, ChinaDepartment of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Orthopaedic Surgery, The Second Affiliated Hospital of Kunming Medical College, 1 Ma Yuan, Kunming, Yunnan 650101, ChinaDepartment of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, ChinaPercutaneous vertebroplasty (PVP) is widely used in the treatment of painful osteoporotic vertebral compression fractures with the injection of PMMA cement, and the controversy for PMMA damage to the osteoporotic bone tissue and to affect the fractures repairing never stops. 72 old female rabbits, each age 3.0~3.5 y, rabbits were assigned randomly to two groups of thirty-six each; PMMA cement were injected into vertebral body in rabbits via mimic PVP, sacrificed at 1 h, 24 h, 3 d, 7 d, 4 w, and 12 w. The expression VEGF and collagen type I, the tissue response, and repair reaction in the interface between PMMA and bone tissue were observed dynamically with RT-PCR and western blot technique; the osteocalcin expression were studied by immunohistochemistry. Compared with the control group, the expression of collagen I increased at 1 hour and was higher from 24 h to 3 d. From 4 weeks to 12 weeks after injection of PMMA. The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4–12 weeks. The osteocalcin expression continued to increase during 4 to 12 week. PMMA would not cause local bone permanent necrosis, and interface injury repairing cycle could be prolonged in a vertebroplasty.http://dx.doi.org/10.1155/2013/109784
spellingShingle ZhenMing Hu
Gang Zhao
LiJun Wang
Bo Pu
Jie Hao
HanChang Lao
XiaoJun Zhang
Qiang Gan
Wei Jiang
Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty
International Journal of Endocrinology
title Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty
title_full Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty
title_fullStr Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty
title_full_unstemmed Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty
title_short Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty
title_sort related biological research in the interface between bone cement and bone after percutaneous vertebroplasty
url http://dx.doi.org/10.1155/2013/109784
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