Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells
Mast cells are traditionally considered as key effector cells in IgE-mediated allergic diseases. However, the roles of mast cells have also been implicated in diverse physiological and pathological processes. Mast cells are distributed in various organs and tissues of various species. Some of the or...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/8439594 |
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| author | Ruoyu Wang Xiaoqin Yin Hui Zhang Jiwei Wang Lin Chen Jingwen Chen Xiaodong Han Zou Xiang Dongmei Li |
| author_facet | Ruoyu Wang Xiaoqin Yin Hui Zhang Jiwei Wang Lin Chen Jingwen Chen Xiaodong Han Zou Xiang Dongmei Li |
| author_sort | Ruoyu Wang |
| collection | DOAJ |
| description | Mast cells are traditionally considered as key effector cells in IgE-mediated allergic diseases. However, the roles of mast cells have also been implicated in diverse physiological and pathological processes. Mast cells are distributed in various organs and tissues of various species. Some of the organs and tissues, such as testis, skin, and the upper part of the respiratory tract, have a temperature that is lower than the body’s core temperature. The purpose of the present study was to investigate the effects of a lower temperature on the proliferation and degranulation of rat mast cells. Here, we demonstrate that cell growth was retarded at 35°C compared to 37°C for both rat peritoneal mast cells (RPMC) and RBL-2H3, a rat mast cell line. Furthermore, RPMC became more susceptible to degranulation at 35°C compared to 37°C. In contrast, degranulation of RBL-2H3 was not as sensitive to temperature change as RPMC. The functionality of mast cells in unique organs with a lower temperature warrants further analysis. |
| format | Article |
| id | doaj-art-db6712596f354a8084af3a413efbe19a |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-db6712596f354a8084af3a413efbe19a2025-08-20T02:21:17ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/84395948439594Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast CellsRuoyu Wang0Xiaoqin Yin1Hui Zhang2Jiwei Wang3Lin Chen4Jingwen Chen5Xiaodong Han6Zou Xiang7Dongmei Li8Immunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaImmunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaImmunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaImmunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaImmunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaImmunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaImmunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaDepartment of Microbiology and Immunology, Mucosal Immunobiology and Vaccine Research Center, Institute of Biomedicine, University of Gothenburg, 40530 Gothenburg, SwedenImmunology and Reproduction Biology Laboratory and State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, ChinaMast cells are traditionally considered as key effector cells in IgE-mediated allergic diseases. However, the roles of mast cells have also been implicated in diverse physiological and pathological processes. Mast cells are distributed in various organs and tissues of various species. Some of the organs and tissues, such as testis, skin, and the upper part of the respiratory tract, have a temperature that is lower than the body’s core temperature. The purpose of the present study was to investigate the effects of a lower temperature on the proliferation and degranulation of rat mast cells. Here, we demonstrate that cell growth was retarded at 35°C compared to 37°C for both rat peritoneal mast cells (RPMC) and RBL-2H3, a rat mast cell line. Furthermore, RPMC became more susceptible to degranulation at 35°C compared to 37°C. In contrast, degranulation of RBL-2H3 was not as sensitive to temperature change as RPMC. The functionality of mast cells in unique organs with a lower temperature warrants further analysis.http://dx.doi.org/10.1155/2016/8439594 |
| spellingShingle | Ruoyu Wang Xiaoqin Yin Hui Zhang Jiwei Wang Lin Chen Jingwen Chen Xiaodong Han Zou Xiang Dongmei Li Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells Journal of Immunology Research |
| title | Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells |
| title_full | Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells |
| title_fullStr | Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells |
| title_full_unstemmed | Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells |
| title_short | Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells |
| title_sort | effects of a moderately lower temperature on the proliferation and degranulation of rat mast cells |
| url | http://dx.doi.org/10.1155/2016/8439594 |
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