Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, i...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/969404 |
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| author | Nancy Bueno Figueiredo Sílvia Helena Cestari Sandro José Conde Renata Azevedo Melo Luvizotto Maria Teresa De Sibio Denise Perone Maria Lúcia Hirata Katayama Dirce Maria Carraro Helena Paula Brentani Maria Mitzi Brentani Célia Regina Nogueira |
| author_facet | Nancy Bueno Figueiredo Sílvia Helena Cestari Sandro José Conde Renata Azevedo Melo Luvizotto Maria Teresa De Sibio Denise Perone Maria Lúcia Hirata Katayama Dirce Maria Carraro Helena Paula Brentani Maria Mitzi Brentani Célia Regina Nogueira |
| author_sort | Nancy Bueno Figueiredo |
| collection | DOAJ |
| description | It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E2), and suppress genes (TGF-beta) normally inhibited by E2. Since T3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E2 and T3. Several genes were modulated by both E2 and T3 in MCF-7 cells (Student’s t-test, P<0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E2 and T3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E2 and T3. |
| format | Article |
| id | doaj-art-db658c279ada4456a37550e06fa6c16e |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-db658c279ada4456a37550e06fa6c16e2025-08-20T03:24:22ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/969404969404Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell LineNancy Bueno Figueiredo0Sílvia Helena Cestari1Sandro José Conde2Renata Azevedo Melo Luvizotto3Maria Teresa De Sibio4Denise Perone5Maria Lúcia Hirata Katayama6Dirce Maria Carraro7Helena Paula Brentani8Maria Mitzi Brentani9Célia Regina Nogueira10Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, BrazilDepartment of Radiology, Medicine School, University of São Paulo, USP, 01246-903 São Paulo, SP, BrazilResearch Center, AC Camargo Hospital, 01509-900 São Paulo, SP, BrazilResearch Center, AC Camargo Hospital, 01509-900 São Paulo, SP, BrazilResearch Center, AC Camargo Hospital, 01509-900 São Paulo, SP, BrazilDepartment of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, BrazilIt has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E2), and suppress genes (TGF-beta) normally inhibited by E2. Since T3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E2 and T3. Several genes were modulated by both E2 and T3 in MCF-7 cells (Student’s t-test, P<0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E2 and T3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E2 and T3.http://dx.doi.org/10.1155/2014/969404 |
| spellingShingle | Nancy Bueno Figueiredo Sílvia Helena Cestari Sandro José Conde Renata Azevedo Melo Luvizotto Maria Teresa De Sibio Denise Perone Maria Lúcia Hirata Katayama Dirce Maria Carraro Helena Paula Brentani Maria Mitzi Brentani Célia Regina Nogueira Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line The Scientific World Journal |
| title | Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line |
| title_full | Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line |
| title_fullStr | Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line |
| title_full_unstemmed | Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line |
| title_short | Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line |
| title_sort | estrogen responsive genes overlap with triiodothyronine responsive genes in a breast carcinoma cell line |
| url | http://dx.doi.org/10.1155/2014/969404 |
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