A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort
Background: The number of sperm washes to maximize outcomes for intra-uterine insemination has not been well investigated. Therefore, we undertook to compare the pregnancy and live birth rates of triple sperm washing and double sperm washing for density gradient preparation for intrauterine insemina...
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| Format: | Article |
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IMR Press
2022-11-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/49/11/10.31083/j.ceog4911250 |
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| author | Canan Soyer-Calıskan Kaan Hatirnaz Samettin Celik Alper Başbuğ Ebru S. Hatirnaz Safak Hatirnaz Michael H. Dahan |
| author_facet | Canan Soyer-Calıskan Kaan Hatirnaz Samettin Celik Alper Başbuğ Ebru S. Hatirnaz Safak Hatirnaz Michael H. Dahan |
| author_sort | Canan Soyer-Calıskan |
| collection | DOAJ |
| description | Background: The number of sperm washes to maximize outcomes for intra-uterine insemination has not been well investigated. Therefore, we undertook to compare the pregnancy and live birth rates of triple sperm washing and double sperm washing for density gradient preparation for intrauterine insemination (IUI) cycles. Methods: A retrospective cohort study including 279 couples (136 couples with triple sperm washing and 143 couples with double sperm washing) with a diagnosis of unexplained infertility and mild male subfertility who had IUI cycles between April 2015 and April 2017 were evaluated. After overnight incubation of the sperm, subjects underwent either traditional double sperm washing or Triple sperm washing which consists of use of a third gradient and spinning procedure to the conventional double gradient sperm washing in order to obtain a higher quantity of motile sperm. Results: Total sperm count after triple washing was higher than double sperm washing (98.25 ± 62.06 vs. 81.08 ± 31.57; p = 0.003). Positive β-hCG test and live birth per cycle were higher in triple sperm washing (25.8% vs. 13.3%, p = 0.009; 18.4% vs. 9.8%, p = 0.039; respectively) than in the double sperm washing group. Conclusions: The use of motile sperms obtained from triple sperm washing may increase the rates of pregnancy and live birth in IUI cycles of women with unexplained and mild male factor infertility. A prospective randomized study should be undertaken to confirm the results. |
| format | Article |
| id | doaj-art-db58da9ec7414fc29b8ccd9378563064 |
| institution | OA Journals |
| issn | 0390-6663 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-db58da9ec7414fc29b8ccd93785630642025-08-20T02:21:17ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632022-11-01491125010.31083/j.ceog4911250S0390-6663(22)01892-9A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective CohortCanan Soyer-Calıskan0Kaan Hatirnaz1Samettin Celik2Alper Başbuğ3Ebru S. Hatirnaz4Safak Hatirnaz5Michael H. Dahan6Maternity Hospital, Samsun Teaching and Education Hospital, 55090 Samsun, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Science, Ondokuzmayıs University, 55270 Samsun, TurkeyMaternity Hospital, Samsun Teaching and Education Hospital, 55090 Samsun, TurkeyDepartment of Obsterics and Gynecology, Duzce University, 81620 Düzce, TurkeyIVF Center, Medicana Hospital, 34520 Samsun, TurkeyIVF Center, Medicana Hospital, 34520 Samsun, TurkeyDepartment of Obstetrics and Gynecology, School of Medicine, Mc Gill University, Montreal, QC H3A 0G4, CanadaBackground: The number of sperm washes to maximize outcomes for intra-uterine insemination has not been well investigated. Therefore, we undertook to compare the pregnancy and live birth rates of triple sperm washing and double sperm washing for density gradient preparation for intrauterine insemination (IUI) cycles. Methods: A retrospective cohort study including 279 couples (136 couples with triple sperm washing and 143 couples with double sperm washing) with a diagnosis of unexplained infertility and mild male subfertility who had IUI cycles between April 2015 and April 2017 were evaluated. After overnight incubation of the sperm, subjects underwent either traditional double sperm washing or Triple sperm washing which consists of use of a third gradient and spinning procedure to the conventional double gradient sperm washing in order to obtain a higher quantity of motile sperm. Results: Total sperm count after triple washing was higher than double sperm washing (98.25 ± 62.06 vs. 81.08 ± 31.57; p = 0.003). Positive β-hCG test and live birth per cycle were higher in triple sperm washing (25.8% vs. 13.3%, p = 0.009; 18.4% vs. 9.8%, p = 0.039; respectively) than in the double sperm washing group. Conclusions: The use of motile sperms obtained from triple sperm washing may increase the rates of pregnancy and live birth in IUI cycles of women with unexplained and mild male factor infertility. A prospective randomized study should be undertaken to confirm the results.https://www.imrpress.com/journal/CEOG/49/11/10.31083/j.ceog4911250intrauterine inseminationlive birthpregnancyinfertility |
| spellingShingle | Canan Soyer-Calıskan Kaan Hatirnaz Samettin Celik Alper Başbuğ Ebru S. Hatirnaz Safak Hatirnaz Michael H. Dahan A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort Clinical and Experimental Obstetrics & Gynecology intrauterine insemination live birth pregnancy infertility |
| title | A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort |
| title_full | A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort |
| title_fullStr | A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort |
| title_full_unstemmed | A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort |
| title_short | A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort |
| title_sort | comparison of triple and double sperm washing for density gradient preparation in intrauterine insemination cycles when overnight incubation of specimens occurred a retrospective cohort |
| topic | intrauterine insemination live birth pregnancy infertility |
| url | https://www.imrpress.com/journal/CEOG/49/11/10.31083/j.ceog4911250 |
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