Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in Mice
In traditional Persian medicine, Haplophyllum acutifolium (DC.) G.Don has been utilized as a treatment for central nervous system (CNS) disorders with sedative-hypnotic effects, as recommended by Avicenna. This plant is recognized for its pharmacological characteristics stemming from the existence...
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Tehran University of Medical Sciences
2025-06-01
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| Series: | Traditional and Integrative Medicine |
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| Online Access: | https://jtim.tums.ac.ir/index.php/jtim/article/view/1669 |
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| author | Seyedali Hashemi Elnaz Ghorbani Marjan Talebi Mona Khoramjouy Noushin Nikray Babak Gholamine Shamim Sahranavard Mehrdad Faizi |
| author_facet | Seyedali Hashemi Elnaz Ghorbani Marjan Talebi Mona Khoramjouy Noushin Nikray Babak Gholamine Shamim Sahranavard Mehrdad Faizi |
| author_sort | Seyedali Hashemi |
| collection | DOAJ |
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In traditional Persian medicine, Haplophyllum acutifolium (DC.) G.Don has been utilized as a treatment for central nervous system (CNS) disorders with sedative-hypnotic effects, as recommended by Avicenna. This plant is recognized for its pharmacological characteristics stemming from the existence of various bioactive compounds including alkaloids, coumarins, flavonoids, and lignans. However, a comprehensive investigation of the sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant activities of H. acutifolium extracts has yet to be conducted. The present investigation aimed to assess the sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant potential of various extracts obtained from H. acutifolium in NMRI and Swiss mice. Total of 250 mice randomly distributed into specific groups based on the test type. The sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant properties of extracts of H. acutifolium were assessed using various tests, including open field, the pentobarbital-induced sleep, elevated plus maze, forced swimming, maximal electroshock, and pentylenetetrazol tests. Aqueous, methanol and dichloromethane extracts were administrated intraperitoneally at doses of 50, 100, 200 and 400 mg/kg. Additionally, the concentration of quercetin in H. acutifolium aqueous extract was determined using reversed-phase high-performance liquid chromatography (RP-HPLC). Total phenolic (TPC) and flavonoid contents (TFC) were assessed by spectroscopic methods as well. Upon behavioral tests, it was found that the aqueous extract has the biggest sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant effects. TPC and TFC of H. acutifolium hydrolyzed aqueous extract were measured as 61.48 ± 2.34 mg GAE/g and 21.13 ± 3.15 RE/g respectively on the basis of colorimetric findings. The RP-HPLC analysis revealed the average quercetin content of 49.87 ± 0.24 mg/100 g dry matter in the aqueous extract. The findings provide a reliable approach for measuring the quantity of quercetin existing in hydrolyzed H. acutifolium aqueous extract, as well as its neuropharmacological effects.
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| format | Article |
| id | doaj-art-db4a557908d449cba18e29fd7dee6131 |
| institution | Kabale University |
| issn | 2476-5104 2476-5112 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Tehran University of Medical Sciences |
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| series | Traditional and Integrative Medicine |
| spelling | doaj-art-db4a557908d449cba18e29fd7dee61312025-08-20T03:50:25ZengTehran University of Medical SciencesTraditional and Integrative Medicine2476-51042476-51122025-06-0110210.18502/tim.v10i2.19056Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in MiceSeyedali Hashemi0Elnaz Ghorbani1Marjan Talebi2Mona Khoramjouy3Noushin Nikray4Babak Gholamine5Shamim Sahranavard6Mehrdad Faizi7Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranStudent Research Committee, Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranPhytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran In traditional Persian medicine, Haplophyllum acutifolium (DC.) G.Don has been utilized as a treatment for central nervous system (CNS) disorders with sedative-hypnotic effects, as recommended by Avicenna. This plant is recognized for its pharmacological characteristics stemming from the existence of various bioactive compounds including alkaloids, coumarins, flavonoids, and lignans. However, a comprehensive investigation of the sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant activities of H. acutifolium extracts has yet to be conducted. The present investigation aimed to assess the sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant potential of various extracts obtained from H. acutifolium in NMRI and Swiss mice. Total of 250 mice randomly distributed into specific groups based on the test type. The sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant properties of extracts of H. acutifolium were assessed using various tests, including open field, the pentobarbital-induced sleep, elevated plus maze, forced swimming, maximal electroshock, and pentylenetetrazol tests. Aqueous, methanol and dichloromethane extracts were administrated intraperitoneally at doses of 50, 100, 200 and 400 mg/kg. Additionally, the concentration of quercetin in H. acutifolium aqueous extract was determined using reversed-phase high-performance liquid chromatography (RP-HPLC). Total phenolic (TPC) and flavonoid contents (TFC) were assessed by spectroscopic methods as well. Upon behavioral tests, it was found that the aqueous extract has the biggest sedative-hypnotic, anxiolytic, antidepressant, and anticonvulsant effects. TPC and TFC of H. acutifolium hydrolyzed aqueous extract were measured as 61.48 ± 2.34 mg GAE/g and 21.13 ± 3.15 RE/g respectively on the basis of colorimetric findings. The RP-HPLC analysis revealed the average quercetin content of 49.87 ± 0.24 mg/100 g dry matter in the aqueous extract. The findings provide a reliable approach for measuring the quantity of quercetin existing in hydrolyzed H. acutifolium aqueous extract, as well as its neuropharmacological effects. https://jtim.tums.ac.ir/index.php/jtim/article/view/1669AnticonvulsantAnxiolyticHaplophyllum acutifoliumHigh-performance liquid chromatographyQuercetinSedative-hypnotic |
| spellingShingle | Seyedali Hashemi Elnaz Ghorbani Marjan Talebi Mona Khoramjouy Noushin Nikray Babak Gholamine Shamim Sahranavard Mehrdad Faizi Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in Mice Traditional and Integrative Medicine Anticonvulsant Anxiolytic Haplophyllum acutifolium High-performance liquid chromatography Quercetin Sedative-hypnotic |
| title | Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in Mice |
| title_full | Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in Mice |
| title_fullStr | Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in Mice |
| title_full_unstemmed | Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in Mice |
| title_short | Study of Antidepressant, Anxiolytic, Sedative-hypnotic, and Anticonvulsant Activities of Haplophyllum acutifolium (DC.) G.Don Aqueous Extract in Mice |
| title_sort | study of antidepressant anxiolytic sedative hypnotic and anticonvulsant activities of haplophyllum acutifolium dc g don aqueous extract in mice |
| topic | Anticonvulsant Anxiolytic Haplophyllum acutifolium High-performance liquid chromatography Quercetin Sedative-hypnotic |
| url | https://jtim.tums.ac.ir/index.php/jtim/article/view/1669 |
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