Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

Non-viral integrating systems, PhiC31 phage integrase (ϕC31), and Sleeping Beauty transposase (SB), provide an effective method for ex vivo gene delivery into cells. Here, we used a plasmid-encoding GFP and neomycin phosphotransferase along with recognition sequences for both ϕC31 and SB integratin...

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Main Authors: Andrew Wilber, Fernando Ulloa Montoya, Luke Hammer, Branden S. Moriarity, Aron M. Geurts, David A. Largaespada, Catherine M. Verfaillie, R. Scott McIvor, Uma Lakshmipathy
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.4061/2011/717069
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