Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communications

Abstract Effective communication between the mammalian hippocampus and neocortex is essential to certain cognitive‐behavioral tasks critical to survival in a changing environment. Notably, functional synchrony between local field potentials (LFPs) of the ventral hippocampus (vHPC) and the medial pre...

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Main Authors: Kristofer J. Schoepfer, Yiqi Xu, Aaron A. Wilber, Wei Wu, Mohamed Kabbaj
Format: Article
Language:English
Published: Wiley 2020-11-01
Series:Physiological Reports
Subjects:
Online Access:https://doi.org/10.14814/phy2.14646
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author Kristofer J. Schoepfer
Yiqi Xu
Aaron A. Wilber
Wei Wu
Mohamed Kabbaj
author_facet Kristofer J. Schoepfer
Yiqi Xu
Aaron A. Wilber
Wei Wu
Mohamed Kabbaj
author_sort Kristofer J. Schoepfer
collection DOAJ
description Abstract Effective communication between the mammalian hippocampus and neocortex is essential to certain cognitive‐behavioral tasks critical to survival in a changing environment. Notably, functional synchrony between local field potentials (LFPs) of the ventral hippocampus (vHPC) and the medial prefrontal cortex (mPFC) within the theta band (4–12 Hz) underlies innate avoidance behavior during approach‐avoidance conflict tasks in male rodents. However, the physiology of vHPC‐mPFC communications in females remains unestablished. Furthermore, little is known about how mPFC subdivisions functionally interact in the theta band with hippocampal subdivisions in both sexes in the absence of task demand. Given the established roles of biological sex and gonadal hormone status on innate avoidance behaviors and neuronal excitability, here, we characterize the effects of biological sex and female estrous stage on hippocampal‐prefrontal (HPC‐mPFC) theta signaling in freely moving female and male rats. LFPs from vHPC, dorsal hippocampus (dHPC), mPFC‐prelimbic (PrL), and mPFC‐infralimbic (IL) were simultaneously recorded during spontaneous exploration of a familiar arena. Data suggest that theta phase and power in vHPC preferentially synchronize with PrL; conversely, dHPC and IL preferentially synchronize. Males displayed greater vHPC‐PrL theta synchrony than females, despite similar regional frequency band power and inter‐regional coherence. Additionally, several significant estrous‐linked changes in HPC‐mPFC theta dynamics were observed. These findings support the hypothesis that HPC‐mPFC theta signaling is sensitive to both biological sex and female estrous stage. These findings establish novel research avenues concerning sex as a biological variable and effects of gonadal hormone status on HPC‐mPFC network activity as it pertains to threat evaluation biomarkers.
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spelling doaj-art-db44d34ba9444e8fbda0015d7c7779622025-08-20T02:29:38ZengWileyPhysiological Reports2051-817X2020-11-01822n/an/a10.14814/phy2.14646Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communicationsKristofer J. Schoepfer0Yiqi Xu1Aaron A. Wilber2Wei Wu3Mohamed Kabbaj4Department of Biomedical Sciences Florida State University College of Medicine Tallahassee FL USADepartment of Statistics Florida State University Tallahassee FL USADepartment of Psychology Florida State University Tallahassee FL USADepartment of Statistics Florida State University Tallahassee FL USADepartment of Biomedical Sciences Florida State University College of Medicine Tallahassee FL USAAbstract Effective communication between the mammalian hippocampus and neocortex is essential to certain cognitive‐behavioral tasks critical to survival in a changing environment. Notably, functional synchrony between local field potentials (LFPs) of the ventral hippocampus (vHPC) and the medial prefrontal cortex (mPFC) within the theta band (4–12 Hz) underlies innate avoidance behavior during approach‐avoidance conflict tasks in male rodents. However, the physiology of vHPC‐mPFC communications in females remains unestablished. Furthermore, little is known about how mPFC subdivisions functionally interact in the theta band with hippocampal subdivisions in both sexes in the absence of task demand. Given the established roles of biological sex and gonadal hormone status on innate avoidance behaviors and neuronal excitability, here, we characterize the effects of biological sex and female estrous stage on hippocampal‐prefrontal (HPC‐mPFC) theta signaling in freely moving female and male rats. LFPs from vHPC, dorsal hippocampus (dHPC), mPFC‐prelimbic (PrL), and mPFC‐infralimbic (IL) were simultaneously recorded during spontaneous exploration of a familiar arena. Data suggest that theta phase and power in vHPC preferentially synchronize with PrL; conversely, dHPC and IL preferentially synchronize. Males displayed greater vHPC‐PrL theta synchrony than females, despite similar regional frequency band power and inter‐regional coherence. Additionally, several significant estrous‐linked changes in HPC‐mPFC theta dynamics were observed. These findings support the hypothesis that HPC‐mPFC theta signaling is sensitive to both biological sex and female estrous stage. These findings establish novel research avenues concerning sex as a biological variable and effects of gonadal hormone status on HPC‐mPFC network activity as it pertains to threat evaluation biomarkers.https://doi.org/10.14814/phy2.14646estroussex differencestheta oscillations
spellingShingle Kristofer J. Schoepfer
Yiqi Xu
Aaron A. Wilber
Wei Wu
Mohamed Kabbaj
Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communications
Physiological Reports
estrous
sex differences
theta oscillations
title Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communications
title_full Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communications
title_fullStr Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communications
title_full_unstemmed Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communications
title_short Sex differences and effects of the estrous stage on hippocampal‐prefrontal theta communications
title_sort sex differences and effects of the estrous stage on hippocampal prefrontal theta communications
topic estrous
sex differences
theta oscillations
url https://doi.org/10.14814/phy2.14646
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