Customizable large-scale HPLC fraction collection using low-cost 3D printing
High-performance liquid chromatography (HPLC) is an invaluable technique that has been used for many decades for the separation of various molecules. The reproducible collection of eluates from these systems has been significantly improved via its automation by fraction collection systems. Current c...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | HardwareX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468067224001068 |
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| author | William J. Crandall Marco Caputo Lewis Marquez Zachery R. Jarrell Cassandra L. Quave |
| author_facet | William J. Crandall Marco Caputo Lewis Marquez Zachery R. Jarrell Cassandra L. Quave |
| author_sort | William J. Crandall |
| collection | DOAJ |
| description | High-performance liquid chromatography (HPLC) is an invaluable technique that has been used for many decades for the separation of various molecules. The reproducible collection of eluates from these systems has been significantly improved via its automation by fraction collection systems. Current commercially available fraction collectors are not easily customizable, incompatible with other platforms, and come with a large cost barrier making them inaccessible to many researchers. Here we present the efficient construction of a low-cost customizable fraction collector that can easily be paired to any HPLC system. Notably, it supports significantly larger volumes for collection than commercial alternatives. Using a hobbyist-grade three-dimensional (3D) printer (Creality Ender 3 Pro) and aluminum extrusions, the fraction collector can be built for less than $280 USD. An additional graphical user interface (GUI) enables simple programming of the collection methods, requiring no coding experience to operate the collector. The presented fraction collector can be highly customized and use collection vessels as large as 470 mL (80x), facilitating repeated collection at a preparatory scale. The use of this platform will increase the reproducibility of scalable and iterative fraction collection methods while removing the cost barrier and allowing for a high degree of customizability. |
| format | Article |
| id | doaj-art-db3ca2200dbc4ffdb7131ba6dd72cf4e |
| institution | DOAJ |
| issn | 2468-0672 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj-art-db3ca2200dbc4ffdb7131ba6dd72cf4e2025-08-20T02:39:51ZengElsevierHardwareX2468-06722025-03-0121e0061210.1016/j.ohx.2024.e00612Customizable large-scale HPLC fraction collection using low-cost 3D printingWilliam J. Crandall0Marco Caputo1Lewis Marquez2Zachery R. Jarrell3Cassandra L. Quave4Molecular and Systems Pharmacology Program, Emory University, Atlanta, GA, USADepartment of Dermatology, Emory University School of Medicine, Atlanta, GA, USAMolecular and Systems Pharmacology Program, Emory University, Atlanta, GA, USA; Jones Center at Ichauway, Newton, GA, USADivision of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Emory University, Atlanta, GA 30322, USAMolecular and Systems Pharmacology Program, Emory University, Atlanta, GA, USA; Department of Dermatology, Emory University School of Medicine, Atlanta, GA, USA; Corresponding author.High-performance liquid chromatography (HPLC) is an invaluable technique that has been used for many decades for the separation of various molecules. The reproducible collection of eluates from these systems has been significantly improved via its automation by fraction collection systems. Current commercially available fraction collectors are not easily customizable, incompatible with other platforms, and come with a large cost barrier making them inaccessible to many researchers. Here we present the efficient construction of a low-cost customizable fraction collector that can easily be paired to any HPLC system. Notably, it supports significantly larger volumes for collection than commercial alternatives. Using a hobbyist-grade three-dimensional (3D) printer (Creality Ender 3 Pro) and aluminum extrusions, the fraction collector can be built for less than $280 USD. An additional graphical user interface (GUI) enables simple programming of the collection methods, requiring no coding experience to operate the collector. The presented fraction collector can be highly customized and use collection vessels as large as 470 mL (80x), facilitating repeated collection at a preparatory scale. The use of this platform will increase the reproducibility of scalable and iterative fraction collection methods while removing the cost barrier and allowing for a high degree of customizability.http://www.sciencedirect.com/science/article/pii/S2468067224001068HPLCFraction CollectorLow-Cost3D PrintingSample Collection |
| spellingShingle | William J. Crandall Marco Caputo Lewis Marquez Zachery R. Jarrell Cassandra L. Quave Customizable large-scale HPLC fraction collection using low-cost 3D printing HardwareX HPLC Fraction Collector Low-Cost 3D Printing Sample Collection |
| title | Customizable large-scale HPLC fraction collection using low-cost 3D printing |
| title_full | Customizable large-scale HPLC fraction collection using low-cost 3D printing |
| title_fullStr | Customizable large-scale HPLC fraction collection using low-cost 3D printing |
| title_full_unstemmed | Customizable large-scale HPLC fraction collection using low-cost 3D printing |
| title_short | Customizable large-scale HPLC fraction collection using low-cost 3D printing |
| title_sort | customizable large scale hplc fraction collection using low cost 3d printing |
| topic | HPLC Fraction Collector Low-Cost 3D Printing Sample Collection |
| url | http://www.sciencedirect.com/science/article/pii/S2468067224001068 |
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