From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial Health
The aim of this study is to investigate the therapeutic effect of <i>Poria cocos</i> polysaccharide (PCP) on bovine endometritis. Initially, an inflammation model was induced using LPS-treated bovine endometrial epithelial cells (BEND) to identify the differentially expressed genes (DEGs...
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2025-02-01
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| author | Yongxi Huang Pupu Yan Jun Zhu Yinuo Gong Man Liu Haishan Cheng Tilin Yi Fuxian Zhang Xiaolin Yang Yingbing Su Liwei Guo |
| author_facet | Yongxi Huang Pupu Yan Jun Zhu Yinuo Gong Man Liu Haishan Cheng Tilin Yi Fuxian Zhang Xiaolin Yang Yingbing Su Liwei Guo |
| author_sort | Yongxi Huang |
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| description | The aim of this study is to investigate the therapeutic effect of <i>Poria cocos</i> polysaccharide (PCP) on bovine endometritis. Initially, an inflammation model was induced using LPS-treated bovine endometrial epithelial cells (BEND) to identify the differentially expressed genes (DEGs) between the control and LPS groups by transcriptome sequencing, and GO functional annotation and KEGG enrichment analysis were performed. Subsequently, the mechanism of PCP treatment for endometritis was further evaluated using protein immunoblotting and real-time fluorescence quantitative analysis. Finally, the efficacy of PCP in treating endometritis was evaluated using a rat model of endometritis established with a mixed bacterial infection. The results show that transcriptome sequencing identified 4367 DEGs, with enrichment analysis highlighting the primary influences on the cell cycle and apoptosis signaling pathways. Following treatment of BEND with LPS resulted in cell apoptosis and inflammatory response. However, the introduction of PCP intervention significantly inhibited the progression of apoptosis and inflammation. Animal test results indicate that PCP significantly decreases the levels of serum inflammatory in rats suffering from endometritis and enhances antioxidant capacity. Furthermore, it effectively improved uterine swelling and tissue vacuolization caused by bacterial infection. These findings suggest that PCP could alleviate endometritis by modulating the inflammatory response and suppressing cell apoptosis. <i>Poria cocos</i> polysaccharides demonstrate significant potential for applications in immune modulation, anti-inflammatory responses, and antioxidant activities. Their high safety profile makes them suitable candidates as alternative therapeutic agents for the treatment of endometritis in the veterinary field. |
| format | Article |
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| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-db2934a20c7d4c36837496cf92bf4cd32025-08-20T02:11:04ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-02-0147313910.3390/cimb47030139From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial HealthYongxi Huang0Pupu Yan1Jun Zhu2Yinuo Gong3Man Liu4Haishan Cheng5Tilin Yi6Fuxian Zhang7Xiaolin Yang8Yingbing Su9Liwei Guo10College of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou 434025, ChinaThe aim of this study is to investigate the therapeutic effect of <i>Poria cocos</i> polysaccharide (PCP) on bovine endometritis. Initially, an inflammation model was induced using LPS-treated bovine endometrial epithelial cells (BEND) to identify the differentially expressed genes (DEGs) between the control and LPS groups by transcriptome sequencing, and GO functional annotation and KEGG enrichment analysis were performed. Subsequently, the mechanism of PCP treatment for endometritis was further evaluated using protein immunoblotting and real-time fluorescence quantitative analysis. Finally, the efficacy of PCP in treating endometritis was evaluated using a rat model of endometritis established with a mixed bacterial infection. The results show that transcriptome sequencing identified 4367 DEGs, with enrichment analysis highlighting the primary influences on the cell cycle and apoptosis signaling pathways. Following treatment of BEND with LPS resulted in cell apoptosis and inflammatory response. However, the introduction of PCP intervention significantly inhibited the progression of apoptosis and inflammation. Animal test results indicate that PCP significantly decreases the levels of serum inflammatory in rats suffering from endometritis and enhances antioxidant capacity. Furthermore, it effectively improved uterine swelling and tissue vacuolization caused by bacterial infection. These findings suggest that PCP could alleviate endometritis by modulating the inflammatory response and suppressing cell apoptosis. <i>Poria cocos</i> polysaccharides demonstrate significant potential for applications in immune modulation, anti-inflammatory responses, and antioxidant activities. Their high safety profile makes them suitable candidates as alternative therapeutic agents for the treatment of endometritis in the veterinary field.https://www.mdpi.com/1467-3045/47/3/139<i>Poria cocos</i> polysaccharideendometritisNF?B signaling pathwayapoptosistranscriptome analysis |
| spellingShingle | Yongxi Huang Pupu Yan Jun Zhu Yinuo Gong Man Liu Haishan Cheng Tilin Yi Fuxian Zhang Xiaolin Yang Yingbing Su Liwei Guo From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial Health Current Issues in Molecular Biology <i>Poria cocos</i> polysaccharide endometritis NF?B signaling pathway apoptosis transcriptome analysis |
| title | From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial Health |
| title_full | From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial Health |
| title_fullStr | From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial Health |
| title_full_unstemmed | From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial Health |
| title_short | From Genes to Healing: The Protective Mechanisms of <i>Poria cocos</i> Polysaccharide in Endometrial Health |
| title_sort | from genes to healing the protective mechanisms of i poria cocos i polysaccharide in endometrial health |
| topic | <i>Poria cocos</i> polysaccharide endometritis NF?B signaling pathway apoptosis transcriptome analysis |
| url | https://www.mdpi.com/1467-3045/47/3/139 |
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