Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain)
A total of 96 strains of Nostocales (Cyanobacteria) were established from the phyllosphere of the laurel forests in the Canary Islands (Spain) and the Azores (Portugal) using enrichment media lacking combined nitrogen. The strains were characterized by light microscopy and SSU rRNA gene comparisons....
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2024-12-01
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| author | Nereida M. Rancel-Rodríguez Nicole Sausen Carolina P. Reyes Antera Martel Quintana Barbara Melkonian Michael Melkonian |
| author_facet | Nereida M. Rancel-Rodríguez Nicole Sausen Carolina P. Reyes Antera Martel Quintana Barbara Melkonian Michael Melkonian |
| author_sort | Nereida M. Rancel-Rodríguez |
| collection | DOAJ |
| description | A total of 96 strains of Nostocales (Cyanobacteria) were established from the phyllosphere of the laurel forests in the Canary Islands (Spain) and the Azores (Portugal) using enrichment media lacking combined nitrogen. The strains were characterized by light microscopy and SSU rRNA gene comparisons. Morphologically, most strains belonged to two different morphotypes, termed “<i>Nostoc</i>-type” and “<i>Tolypothrix</i>-type”. Molecular phylogenetic analysis of 527 SSU rRNA gene sequences of cyanobacteria (95 sequences established during this study plus 392 sequences from Nostocales and 40 sequences from non-heterocyte-forming cyanobacteria retrieved from the databases) revealed that none of the SSU rRNA gene sequences from the phyllosphere of the laurel forests was identical to a database sequence. In addition, the genetic diversity of the isolated strains was high, with 42 different genotypes (44% of the sequences) recognized. Among the new genotypes were also terrestrial members of the genus <i>Nodularia</i> as well as members of the genus <i>Brasilonema</i>. It is concluded that heterocyte-forming cyanobacteria represent a component of the phyllosphere that is still largely undersampled in subtropical/tropical forests. |
| format | Article |
| id | doaj-art-db2451e5ca024742ae73d474fbfe04ea |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-db2451e5ca024742ae73d474fbfe04ea2025-08-20T02:56:59ZengMDPI AGMicroorganisms2076-26072024-12-011212262510.3390/microorganisms12122625Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain)Nereida M. Rancel-Rodríguez0Nicole Sausen1Carolina P. Reyes2Antera Martel Quintana3Barbara Melkonian4Michael Melkonian5Departamento de Botánica, Ecología y Fisiología Vegetal, Universidad de La Laguna, 38200 San Cristóbal De La Laguna, SpainInstitute for Plant Sciences, Department of Biology, University of Cologne, Zülpicher Str. 47b, 50674 Cologne, GermanyInstituto Universitario de Bio-Orgánica “Antonio González”, Universidad de La Laguna, 38206 San Cristóbal De La Laguna, SpainBanco Español de Algas, Instituto de Oceanografía y Cambio Global, IOCAG, Universidad de Las Palmas de Gran Canaria, 35214 Telde, SpainIntegrative Bioinformatics, Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, GermanyIntegrative Bioinformatics, Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, GermanyA total of 96 strains of Nostocales (Cyanobacteria) were established from the phyllosphere of the laurel forests in the Canary Islands (Spain) and the Azores (Portugal) using enrichment media lacking combined nitrogen. The strains were characterized by light microscopy and SSU rRNA gene comparisons. Morphologically, most strains belonged to two different morphotypes, termed “<i>Nostoc</i>-type” and “<i>Tolypothrix</i>-type”. Molecular phylogenetic analysis of 527 SSU rRNA gene sequences of cyanobacteria (95 sequences established during this study plus 392 sequences from Nostocales and 40 sequences from non-heterocyte-forming cyanobacteria retrieved from the databases) revealed that none of the SSU rRNA gene sequences from the phyllosphere of the laurel forests was identical to a database sequence. In addition, the genetic diversity of the isolated strains was high, with 42 different genotypes (44% of the sequences) recognized. Among the new genotypes were also terrestrial members of the genus <i>Nodularia</i> as well as members of the genus <i>Brasilonema</i>. It is concluded that heterocyte-forming cyanobacteria represent a component of the phyllosphere that is still largely undersampled in subtropical/tropical forests.https://www.mdpi.com/2076-2607/12/12/2625biodiversityclonal cultureepiphyllousheterocyte-forming cyanobacteriarRNA sequence comparison |
| spellingShingle | Nereida M. Rancel-Rodríguez Nicole Sausen Carolina P. Reyes Antera Martel Quintana Barbara Melkonian Michael Melkonian Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain) Microorganisms biodiversity clonal culture epiphyllous heterocyte-forming cyanobacteria rRNA sequence comparison |
| title | Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain) |
| title_full | Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain) |
| title_fullStr | Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain) |
| title_full_unstemmed | Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain) |
| title_short | Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain) |
| title_sort | unexpected genetic diversity of nostocales cyanobacteria isolated from the phyllosphere of the laurel forests in the canary islands spain |
| topic | biodiversity clonal culture epiphyllous heterocyte-forming cyanobacteria rRNA sequence comparison |
| url | https://www.mdpi.com/2076-2607/12/12/2625 |
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