Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats
<i>Fagonia cretica</i>, a medicinal herb from the Zygophyllaceae family, is traditionally utilized to treat various conditions such as hepatitis, gynecological disorders, tumors, urinary tract issues, and diabetes. The present study aimed to evaluate the therapeutic potential of <i>...
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2025-07-01
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| author | Ayesha Qasim Hiram Calvo Jesús Jaime Moreno Escobar Zia-ud-din Akhtar |
| author_facet | Ayesha Qasim Hiram Calvo Jesús Jaime Moreno Escobar Zia-ud-din Akhtar |
| author_sort | Ayesha Qasim |
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| description | <i>Fagonia cretica</i>, a medicinal herb from the Zygophyllaceae family, is traditionally utilized to treat various conditions such as hepatitis, gynecological disorders, tumors, urinary tract issues, and diabetes. The present study aimed to evaluate the therapeutic potential of <i>Fagonia cretica</i> in treating polycystic ovarian syndrome (PCOS) induced in female rats. PCOS, a complex hormonal disorder, was experimentally induced by administering Letrozole (1 mg/kg) in combination with a high-fat diet for 21 days. The affected rats were then treated with hydro-alcoholic extracts of <i>Fagonia cretica</i> at doses of 100 mg/kg, 200 mg/kg, and 300 mg/kg for 20 days. Key biochemical parameters—including serum testosterone, insulin, fasting blood glucose, insulin resistance (HOMA-IR), cholesterol, triglycerides, and lipoprotein levels—were measured. Ultrasound imaging and histopathological analysis of ovarian tissues were also performed. The data were analyzed using computer-based statistical tools, including one-way ANOVA, Cohen’s d effect size, and Tukey’s HSD test, with graphical representations generated using Python 3.10 on the Kaggle platform. Results demonstrated a significant reduction in serum testosterone, insulin, cholesterol, and triglyceride levels (<i>p</i> < 0.05) in treated groups, along with improved ovarian morphology. These findings support the therapeutic potential of <i>Fagonia cretica</i> as a natural treatment for PCOS. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-dafd9b09ee474be3be6773a91f0e2cc82025-08-20T03:35:27ZengMDPI AGBiology2079-77372025-07-0114790310.3390/biology14070903Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female RatsAyesha Qasim0Hiram Calvo1Jesús Jaime Moreno Escobar2Zia-ud-din Akhtar3Centro de Investigación en Computación, Instituto Politécnico Nacional, Ciudad de México 07700, MexicoCentro de Investigación en Computación, Instituto Politécnico Nacional, Ciudad de México 07700, MexicoCentro de Investigación en Computación, Instituto Politécnico Nacional, Ciudad de México 07700, MexicoCentro de Investigación en Computación, Instituto Politécnico Nacional, Ciudad de México 07700, Mexico<i>Fagonia cretica</i>, a medicinal herb from the Zygophyllaceae family, is traditionally utilized to treat various conditions such as hepatitis, gynecological disorders, tumors, urinary tract issues, and diabetes. The present study aimed to evaluate the therapeutic potential of <i>Fagonia cretica</i> in treating polycystic ovarian syndrome (PCOS) induced in female rats. PCOS, a complex hormonal disorder, was experimentally induced by administering Letrozole (1 mg/kg) in combination with a high-fat diet for 21 days. The affected rats were then treated with hydro-alcoholic extracts of <i>Fagonia cretica</i> at doses of 100 mg/kg, 200 mg/kg, and 300 mg/kg for 20 days. Key biochemical parameters—including serum testosterone, insulin, fasting blood glucose, insulin resistance (HOMA-IR), cholesterol, triglycerides, and lipoprotein levels—were measured. Ultrasound imaging and histopathological analysis of ovarian tissues were also performed. The data were analyzed using computer-based statistical tools, including one-way ANOVA, Cohen’s d effect size, and Tukey’s HSD test, with graphical representations generated using Python 3.10 on the Kaggle platform. Results demonstrated a significant reduction in serum testosterone, insulin, cholesterol, and triglyceride levels (<i>p</i> < 0.05) in treated groups, along with improved ovarian morphology. These findings support the therapeutic potential of <i>Fagonia cretica</i> as a natural treatment for PCOS.https://www.mdpi.com/2079-7737/14/7/903<i>Fagonia cretica</i>PCOSletrozole induced PCOSmodulatory effectin vivo investigationhydro-alcoholic |
| spellingShingle | Ayesha Qasim Hiram Calvo Jesús Jaime Moreno Escobar Zia-ud-din Akhtar Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats Biology <i>Fagonia cretica</i> PCOS letrozole induced PCOS modulatory effect in vivo investigation hydro-alcoholic |
| title | Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats |
| title_full | Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats |
| title_fullStr | Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats |
| title_full_unstemmed | Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats |
| title_short | Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of <i>Fagonia cretica</i> Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats |
| title_sort | integrating computational analysis of in vivo investigation of modulatory effect of i fagonia cretica i plant extract on letrozole induced polycystic ovary syndrome in female rats |
| topic | <i>Fagonia cretica</i> PCOS letrozole induced PCOS modulatory effect in vivo investigation hydro-alcoholic |
| url | https://www.mdpi.com/2079-7737/14/7/903 |
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