Brain metabolites in ISIAH and Wistar rats
Hypertension is one of the most common human diseases. This disease leads to serious disturbances such as myocardial infarction and stroke. Due to the development of nuclear magnetic resonance spectroscopy (NMRS), a decrease in neuron viability in different parts of the brain in humans with hyperten...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-12-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/430 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575259931312128 |
|---|---|
| author | O. B. Shevelev A. A. Seryapina A. L. Markel M. P. Moshkin |
| author_facet | O. B. Shevelev A. A. Seryapina A. L. Markel M. P. Moshkin |
| author_sort | O. B. Shevelev |
| collection | DOAJ |
| description | Hypertension is one of the most common human diseases. This disease leads to serious disturbances such as myocardial infarction and stroke. Due to the development of nuclear magnetic resonance spectroscopy (NMRS), a decrease in neuron viability in different parts of the brain in humans with hypertension has been shown. Translation of NMRS tools to the clinic requires the accumulation of empirical data about neurometabolic changes in a strictly controlled experiment. It is particularly interesting to compare the metabolic parameters of laboratory animals with normal and high blood pressure kept in standard conditions on exactly the same diet. In this study, cortex and hypothalamus metabolites of ISIAH and Wistar male rats at the age of 8–9 weeks were examined. Cortex and hypothalamus metabolites were measured in animals under isoflurane anesthesia using proton magnetic resonance spectroscopy (1Н MRS). Processing of primary data using Partial least squares Discriminant Analysis (PLS-DA) allowed us to identify the main discriminating axis (Y1), its variations reflecting the predominance of excitatory neurometabolites (glutamine and glutamate) over inhibitory ones (GABA and glycine). In the cortex, the values of the Y1-axis were lower in ISIAH than in Wistar rats. This fact indicates a decrease in cortical excitability in hypertensive animals. By contrast, in the hypothalamus, the values of the Y1-axis were higher in ISIAH than in Wistar rats and the predominance of excitatory neurometabolites positively correlated with the level of mean blood pressure, which agrees well with the view of caudal hypothalamic activation in hypertensive animal models. |
| format | Article |
| id | doaj-art-dafd5b8235224175ad2be7882c36f314 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-dafd5b8235224175ad2be7882c36f3142025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119442743110.18699/VJ15.056389Brain metabolites in ISIAH and Wistar ratsO. B. Shevelev0A. A. Seryapina1A. L. Markel2M. P. Moshkin3Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaHypertension is one of the most common human diseases. This disease leads to serious disturbances such as myocardial infarction and stroke. Due to the development of nuclear magnetic resonance spectroscopy (NMRS), a decrease in neuron viability in different parts of the brain in humans with hypertension has been shown. Translation of NMRS tools to the clinic requires the accumulation of empirical data about neurometabolic changes in a strictly controlled experiment. It is particularly interesting to compare the metabolic parameters of laboratory animals with normal and high blood pressure kept in standard conditions on exactly the same diet. In this study, cortex and hypothalamus metabolites of ISIAH and Wistar male rats at the age of 8–9 weeks were examined. Cortex and hypothalamus metabolites were measured in animals under isoflurane anesthesia using proton magnetic resonance spectroscopy (1Н MRS). Processing of primary data using Partial least squares Discriminant Analysis (PLS-DA) allowed us to identify the main discriminating axis (Y1), its variations reflecting the predominance of excitatory neurometabolites (glutamine and glutamate) over inhibitory ones (GABA and glycine). In the cortex, the values of the Y1-axis were lower in ISIAH than in Wistar rats. This fact indicates a decrease in cortical excitability in hypertensive animals. By contrast, in the hypothalamus, the values of the Y1-axis were higher in ISIAH than in Wistar rats and the predominance of excitatory neurometabolites positively correlated with the level of mean blood pressure, which agrees well with the view of caudal hypothalamic activation in hypertensive animal models.https://vavilov.elpub.ru/jour/article/view/430brain metabolites1h mrshypertensionreishi |
| spellingShingle | O. B. Shevelev A. A. Seryapina A. L. Markel M. P. Moshkin Brain metabolites in ISIAH and Wistar rats Вавиловский журнал генетики и селекции brain metabolites 1h mrs hypertension reishi |
| title | Brain metabolites in ISIAH and Wistar rats |
| title_full | Brain metabolites in ISIAH and Wistar rats |
| title_fullStr | Brain metabolites in ISIAH and Wistar rats |
| title_full_unstemmed | Brain metabolites in ISIAH and Wistar rats |
| title_short | Brain metabolites in ISIAH and Wistar rats |
| title_sort | brain metabolites in isiah and wistar rats |
| topic | brain metabolites 1h mrs hypertension reishi |
| url | https://vavilov.elpub.ru/jour/article/view/430 |
| work_keys_str_mv | AT obshevelev brainmetabolitesinisiahandwistarrats AT aaseryapina brainmetabolitesinisiahandwistarrats AT almarkel brainmetabolitesinisiahandwistarrats AT mpmoshkin brainmetabolitesinisiahandwistarrats |