Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration
This study explores the efficacy of a vancomycin-loaded silk fibroin/calcium phosphate/methylcellulose-based in situ thermosensitive hydrogel (VM-SF/CaP/MC) in promoting the osteogenic differentiation of preosteoblast cells. Three VM-SF/CaP/MC formulations with varying low (L) and high (H) concentra...
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MDPI AG
2024-10-01
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| author | Premchirakorn Phewchan Artit Laoruengthana Pratthana Chomchalao Supaporn Lamlertthon Waree Tiyaboonchai |
| author_facet | Premchirakorn Phewchan Artit Laoruengthana Pratthana Chomchalao Supaporn Lamlertthon Waree Tiyaboonchai |
| author_sort | Premchirakorn Phewchan |
| collection | DOAJ |
| description | This study explores the efficacy of a vancomycin-loaded silk fibroin/calcium phosphate/methylcellulose-based in situ thermosensitive hydrogel (VM-SF/CaP/MC) in promoting the osteogenic differentiation of preosteoblast cells. Three VM-SF/CaP/MC formulations with varying low (L) and high (H) concentrations of silk fibroin (SF) and calcium phosphate (CaP) were prepared: VM-HSF/LCaP/MC, VM-LSF/HCaP/MC, and VM-HSF/HCaP/MC. These hydrogels significantly enhanced MC3T3-E1 cell migration and proliferation in a dose- and time-dependent manner, achieving complete cell migration within 48 h. In addition, they significantly promoted alkaline phosphatase activity, collagen content, and mineralization in MC3T3-E1 cells, indicating their potential for osteogenesis. Among the hydrogel formulations, the VM-HSF/HCaP/MC hydrogel, with high SF and CaP content, demonstrated superior potential in promoting the osteogenic differentiation of MC3T3-E1 cells. It exhibited the highest ALP activity (11.13 ± 0.91 U/mg protein) over 14 days, along with increased collagen content (54.00 ± 1.71 µg/mg protein) and mineralization (15.79 ± 1.48 mM) over 35 days. Therefore, this formulation showed a promising candidate for clinical application in localized bone regeneration, particularly in treating osteomyelitis. |
| format | Article |
| id | doaj-art-dafc3d70a3f7403fbaae69af01da7a0f |
| institution | OA Journals |
| issn | 2310-2861 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Gels |
| spelling | doaj-art-dafc3d70a3f7403fbaae69af01da7a0f2025-08-20T02:04:57ZengMDPI AGGels2310-28612024-10-01101169510.3390/gels10110695Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone RegenerationPremchirakorn Phewchan0Artit Laoruengthana1Pratthana Chomchalao2Supaporn Lamlertthon3Waree Tiyaboonchai4Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, ThailandDepartment of Orthopedics, Faculty of Medicine, Naresuan University, Phitsanulok 65000, ThailandCollege of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Sciences, Naresuan University, Phitsanulok 65000, ThailandDepartment of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, ThailandThis study explores the efficacy of a vancomycin-loaded silk fibroin/calcium phosphate/methylcellulose-based in situ thermosensitive hydrogel (VM-SF/CaP/MC) in promoting the osteogenic differentiation of preosteoblast cells. Three VM-SF/CaP/MC formulations with varying low (L) and high (H) concentrations of silk fibroin (SF) and calcium phosphate (CaP) were prepared: VM-HSF/LCaP/MC, VM-LSF/HCaP/MC, and VM-HSF/HCaP/MC. These hydrogels significantly enhanced MC3T3-E1 cell migration and proliferation in a dose- and time-dependent manner, achieving complete cell migration within 48 h. In addition, they significantly promoted alkaline phosphatase activity, collagen content, and mineralization in MC3T3-E1 cells, indicating their potential for osteogenesis. Among the hydrogel formulations, the VM-HSF/HCaP/MC hydrogel, with high SF and CaP content, demonstrated superior potential in promoting the osteogenic differentiation of MC3T3-E1 cells. It exhibited the highest ALP activity (11.13 ± 0.91 U/mg protein) over 14 days, along with increased collagen content (54.00 ± 1.71 µg/mg protein) and mineralization (15.79 ± 1.48 mM) over 35 days. Therefore, this formulation showed a promising candidate for clinical application in localized bone regeneration, particularly in treating osteomyelitis.https://www.mdpi.com/2310-2861/10/11/695silk fibroincalcium phosphateMC3T3-E1 cellsosteogenic differentiationosteomyelitisbone regeneration |
| spellingShingle | Premchirakorn Phewchan Artit Laoruengthana Pratthana Chomchalao Supaporn Lamlertthon Waree Tiyaboonchai Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration Gels silk fibroin calcium phosphate MC3T3-E1 cells osteogenic differentiation osteomyelitis bone regeneration |
| title | Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration |
| title_full | Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration |
| title_fullStr | Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration |
| title_full_unstemmed | Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration |
| title_short | Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration |
| title_sort | vancomycin loaded silk fibroin calcium phosphate methylcellulose based in situ thermosensitive hydrogel a potential function for bone regeneration |
| topic | silk fibroin calcium phosphate MC3T3-E1 cells osteogenic differentiation osteomyelitis bone regeneration |
| url | https://www.mdpi.com/2310-2861/10/11/695 |
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