Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens

Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens

ABSTRACT Long-read metagenomics provides a promising alternative approach to fungal identification, circumventing methodological biases, associated with DNA amplification, which is a prerequisite for DNA barcoding/metabarcoding based on the primary fungal DNA barcode (Internal Transcribed Spacer (IT...

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Main Authors: Nattapong Langsiri, Wieland Meyer, Laszlo Irinyi, Navaporn Worasilchai, Nuttapon Pombubpa, Thidathip Wongsurawat, Piroon Jenjaroenpun, J. Jennifer Luangsa-ard, Ariya Chindamporn
Format: Article
Language:English
Published: American Society for Microbiology 2025-06-01
Series:mSystems
Subjects:
DNA preparation
long read
simulated hemoculture
Oxford Nanopore Technology
Online Access:https://journals.asm.org/doi/10.1128/msystems.01166-24
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https://journals.asm.org/doi/10.1128/msystems.01166-24

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