The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats

Objective: to examine the effect of erythropoietin testing on rat model and particularly the ischemia reperfusion protocol. Methods: experimental study of 40 rats weighing 247.7 g as average. The beneficial effect or non-effectiveness of the erythroproietin molecule on the blood creatine phosphokina...

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Main Authors: Constantinos Tsompos, Constantinos Panoulis, Konstantinos Toutouzas, George Zografos, Apostolos Papalois
Format: Article
Language:Spanish
Published: Editorial Ciencias Médicas - ECIMED
Series:Revista Cubana de Medicina Militar
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Online Access:http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0138-65572014000300002&lng=en&tlng=en
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author Constantinos Tsompos
Constantinos Panoulis
Konstantinos Toutouzas
George Zografos
Apostolos Papalois
author_facet Constantinos Tsompos
Constantinos Panoulis
Konstantinos Toutouzas
George Zografos
Apostolos Papalois
author_sort Constantinos Tsompos
collection DOAJ
description Objective: to examine the effect of erythropoietin testing on rat model and particularly the ischemia reperfusion protocol. Methods: experimental study of 40 rats weighing 247.7 g as average. The beneficial effect or non-effectiveness of the erythroproietin molecule on the blood creatine phosphokinase levels was biochemically studied. It was measured 60 min (groups A and C) and 120 min (groups B and D) after reperfusion with no administration of erythropoietin in groups C and D. Results: Erythropoietin administration significantly increased the creatine phosphokinase levels to 3586.05 IU/L (1873.115 IU/L-5298.985 IU/L;p= 0.0001). This finding was in accordance with the result of paired t-test (p= 0.0001). Reperfusion time significantly increased the CPK levels to 557.35 IU/L (-1513.284 IU/L-2627.984 IU/L; p= 0.5890), also in accordance with paired t-test (p= 0.4661). The interaction of erythropoietin administration and reperfusion time significantly increased the creatine phosphokinase levels to 1988.282 IU/L (918.2646 IU/L-3058.299 IU/L; p= 0.0006). Conclusions: Erythropoietin administration, reperfusion time and their interaction generally increase short-term effects on blood creatine phosphokinase after ischemia reperfusion injury.
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spelling doaj-art-daf2f75e523e4f0d8e6fd9e3584995892025-08-20T03:09:58ZspaEditorial Ciencias Médicas - ECIMEDRevista Cubana de Medicina Militar1561-3046433277284S0138-65572014000300002The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in ratsConstantinos Tsompos0Constantinos Panoulis1Konstantinos Toutouzas2George Zografos3Apostolos Papalois4Mesologi County HospitalAthens UniversityAthens UniversityAthens UniversityExperimental Research CenterObjective: to examine the effect of erythropoietin testing on rat model and particularly the ischemia reperfusion protocol. Methods: experimental study of 40 rats weighing 247.7 g as average. The beneficial effect or non-effectiveness of the erythroproietin molecule on the blood creatine phosphokinase levels was biochemically studied. It was measured 60 min (groups A and C) and 120 min (groups B and D) after reperfusion with no administration of erythropoietin in groups C and D. Results: Erythropoietin administration significantly increased the creatine phosphokinase levels to 3586.05 IU/L (1873.115 IU/L-5298.985 IU/L;p= 0.0001). This finding was in accordance with the result of paired t-test (p= 0.0001). Reperfusion time significantly increased the CPK levels to 557.35 IU/L (-1513.284 IU/L-2627.984 IU/L; p= 0.5890), also in accordance with paired t-test (p= 0.4661). The interaction of erythropoietin administration and reperfusion time significantly increased the creatine phosphokinase levels to 1988.282 IU/L (918.2646 IU/L-3058.299 IU/L; p= 0.0006). Conclusions: Erythropoietin administration, reperfusion time and their interaction generally increase short-term effects on blood creatine phosphokinase after ischemia reperfusion injury.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0138-65572014000300002&lng=en&tlng=eneritropoyetinacreatín fosfoquinasareperfusión
spellingShingle Constantinos Tsompos
Constantinos Panoulis
Konstantinos Toutouzas
George Zografos
Apostolos Papalois
The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats
Revista Cubana de Medicina Militar
eritropoyetina
creatín fosfoquinasa
reperfusión
title The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats
title_full The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats
title_fullStr The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats
title_full_unstemmed The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats
title_short The effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats
title_sort effect of erythropoietin on creatine phosphokinase levels during ischemia reperfusion injury in rats
topic eritropoyetina
creatín fosfoquinasa
reperfusión
url http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0138-65572014000300002&lng=en&tlng=en
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