Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensis
The tea plant cultivar ‘Zhonghuang 2’ (ZH2) possesses albino-induced yellow leaves that contain low levels of catechins but high contents of amino acids. However, the molecular mechanism underlying the yellow leaf phenotype of ZH2 has not been elucidated clearly. In the current research, the yellow...
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KeAi Communications Co., Ltd.
2025-01-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S246801412400044X |
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| author | Nana Li Weizhong He Yufan Ye Mingming He Taimei Di Xinyuan Hao Changqing Ding Yajun Yang Lu Wang Xinchao Wang |
| author_facet | Nana Li Weizhong He Yufan Ye Mingming He Taimei Di Xinyuan Hao Changqing Ding Yajun Yang Lu Wang Xinchao Wang |
| author_sort | Nana Li |
| collection | DOAJ |
| description | The tea plant cultivar ‘Zhonghuang 2’ (ZH2) possesses albino-induced yellow leaves that contain low levels of catechins but high contents of amino acids. However, the molecular mechanism underlying the yellow leaf phenotype of ZH2 has not been elucidated clearly. In the current research, the yellow shoots (ZH2-Y) and naturally converted green shoots (ZH2-G) of ZH2 were studied using metabolic and proteomic profiling for a better understanding of the mechanism underlying phenotype formation. In total, 107 differentially changed metabolites (DCMs) were identified from the GC‒MS-based metabolomics, and 189 differentially accumulated proteins (DAPs) were identified from the tandem mass tag (TMT)-based quantitative proteomics. Subsequently, integrated analysis revealed that ‘porphyrin and chlorophyll metabolism’, ‘carbon fixation in photosynthetic organisms’, and ‘phenylpropanoid biosynthesis’ pathways were commonly enriched for DAPs and DCMs. We further found that the inhibition of chlorophyll biosynthesis, the deficiency of photosynthetic proteins and the imbalance of the ROS-scavenging system were the crucial reasons responsible for the chlorosis, chloroplast abnormality and photooxidative damage of ZH2 leaves. Altogether, our research combines metabolomics and proteomics approaches to uncover the molecular mechanism leading to the yellow leaf phenotype of tea plants. |
| format | Article |
| id | doaj-art-daf0333fb09f43e08e4b90c77d508c14 |
| institution | DOAJ |
| issn | 2468-0141 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj-art-daf0333fb09f43e08e4b90c77d508c142025-08-20T02:51:18ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412025-01-0111141743010.1016/j.hpj.2023.07.010Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensisNana Li0Weizhong He1Yufan Ye2Mingming He3Taimei Di4Xinyuan Hao5Changqing Ding6Yajun Yang7Lu Wang8Xinchao Wang9National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Lishui Academy of Agricultural and Forestry Sciences, Lishui, Zhejiang 323000, ChinaNational Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, China; College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, Fujian 350002, ChinaNational Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaNational Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaNational Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaNational Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaNational Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaNational Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, China; Corresponding authors.National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, China; Corresponding authors.The tea plant cultivar ‘Zhonghuang 2’ (ZH2) possesses albino-induced yellow leaves that contain low levels of catechins but high contents of amino acids. However, the molecular mechanism underlying the yellow leaf phenotype of ZH2 has not been elucidated clearly. In the current research, the yellow shoots (ZH2-Y) and naturally converted green shoots (ZH2-G) of ZH2 were studied using metabolic and proteomic profiling for a better understanding of the mechanism underlying phenotype formation. In total, 107 differentially changed metabolites (DCMs) were identified from the GC‒MS-based metabolomics, and 189 differentially accumulated proteins (DAPs) were identified from the tandem mass tag (TMT)-based quantitative proteomics. Subsequently, integrated analysis revealed that ‘porphyrin and chlorophyll metabolism’, ‘carbon fixation in photosynthetic organisms’, and ‘phenylpropanoid biosynthesis’ pathways were commonly enriched for DAPs and DCMs. We further found that the inhibition of chlorophyll biosynthesis, the deficiency of photosynthetic proteins and the imbalance of the ROS-scavenging system were the crucial reasons responsible for the chlorosis, chloroplast abnormality and photooxidative damage of ZH2 leaves. Altogether, our research combines metabolomics and proteomics approaches to uncover the molecular mechanism leading to the yellow leaf phenotype of tea plants.http://www.sciencedirect.com/science/article/pii/S246801412400044XTea plantChlorophyll-deficient mutantChlorophyll biosynthesisPhotosynthetic proteinAntioxidant capacityPhotooxidative stress |
| spellingShingle | Nana Li Weizhong He Yufan Ye Mingming He Taimei Di Xinyuan Hao Changqing Ding Yajun Yang Lu Wang Xinchao Wang Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensis Horticultural Plant Journal Tea plant Chlorophyll-deficient mutant Chlorophyll biosynthesis Photosynthetic protein Antioxidant capacity Photooxidative stress |
| title | Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensis |
| title_full | Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensis |
| title_fullStr | Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensis |
| title_full_unstemmed | Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensis |
| title_short | Integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of Camellia sinensis |
| title_sort | integrated metabolomics and proteomics analyses reveal the molecular mechanism underlying the yellow leaf phenotype of camellia sinensis |
| topic | Tea plant Chlorophyll-deficient mutant Chlorophyll biosynthesis Photosynthetic protein Antioxidant capacity Photooxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S246801412400044X |
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