Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida
A bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess toxicity of the environmentally relevant concentrations of mercury species (MeHg and Hg(II)) that represent important pollutants of aquatic ecosystems at sites of industrial or mining activities. In addition...
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| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2010-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/14702 |
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| author | Maša VODOVNIK Mirjana BISTAN Maša ZOREC Romana MARINŠEK LOGAR |
| author_facet | Maša VODOVNIK Mirjana BISTAN Maša ZOREC Romana MARINŠEK LOGAR |
| author_sort | Maša VODOVNIK |
| collection | DOAJ |
| description | A bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess toxicity of the environmentally relevant concentrations of mercury species (MeHg and Hg(II)) that represent important pollutants of aquatic ecosystems at sites of industrial or mining activities. In addition to direct monitoring of bacterial growth, we also analyzed fatty acid profiles of exposed and non-exposed cultures to determine possible toxic effects manifested on membrane level. The results showed that exposure of P. putida to Hg(II) in concentrations of 0.2–200 µg/L did not have any significant effects on growth nor fatty acid composition of exposed bacterial culture. On the other hand, when bacteria were exposed to up to 1600-times lower concentrations of MeHg (0.12–12.5 µg/L), growth inhibition as well as significant changes in fatty acid composition were detected. Observed adaptive membrane changes due to MeHg exposure were similar to those associated with responses to organic solvents and some other membrane-disrupting compounds. |
| format | Article |
| id | doaj-art-dae69b2e09a742a8a5991f132f5e989c |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2010-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-dae69b2e09a742a8a5991f132f5e989c2025-08-20T02:58:55ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412010-12-01962879310.14720/aas.2010.96.2.1470221094Methylmercury inhibits growth and induces membrane changes in Pseudomonas putidaMaša VODOVNIK0Mirjana BISTAN1Maša ZOREC2Romana MARINŠEK LOGAR3Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaLaboratory for environmental science and engineering, National institute of chemistry, Hajdrihova 19, SI-1000 Ljubljana, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaA bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess toxicity of the environmentally relevant concentrations of mercury species (MeHg and Hg(II)) that represent important pollutants of aquatic ecosystems at sites of industrial or mining activities. In addition to direct monitoring of bacterial growth, we also analyzed fatty acid profiles of exposed and non-exposed cultures to determine possible toxic effects manifested on membrane level. The results showed that exposure of P. putida to Hg(II) in concentrations of 0.2–200 µg/L did not have any significant effects on growth nor fatty acid composition of exposed bacterial culture. On the other hand, when bacteria were exposed to up to 1600-times lower concentrations of MeHg (0.12–12.5 µg/L), growth inhibition as well as significant changes in fatty acid composition were detected. Observed adaptive membrane changes due to MeHg exposure were similar to those associated with responses to organic solvents and some other membrane-disrupting compounds.https://journals.uni-lj.si/aas/article/view/14702microbiologyenvironmental protectionbacteriapseudomonas putidaaquatic ecosystemspollutionmercurymethylmercurygrowth inhibitionmembrane adaptationcis-trans isomerization |
| spellingShingle | Maša VODOVNIK Mirjana BISTAN Maša ZOREC Romana MARINŠEK LOGAR Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida Acta Agriculturae Slovenica microbiology environmental protection bacteria pseudomonas putida aquatic ecosystems pollution mercury methylmercury growth inhibition membrane adaptation cis-trans isomerization |
| title | Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida |
| title_full | Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida |
| title_fullStr | Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida |
| title_full_unstemmed | Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida |
| title_short | Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida |
| title_sort | methylmercury inhibits growth and induces membrane changes in pseudomonas putida |
| topic | microbiology environmental protection bacteria pseudomonas putida aquatic ecosystems pollution mercury methylmercury growth inhibition membrane adaptation cis-trans isomerization |
| url | https://journals.uni-lj.si/aas/article/view/14702 |
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