Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing Technology
Tuberculosis (TB) remains the leading cause of human mortality in infectious diseases. Drug-resistant TB, particularly multidrug-resistant TB and extensively drug-resistant TB, poses a pressing clinical and public health challenge. The main causative agents of TB are known as Mycobacterium tuberculo...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-02-01
|
| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5170&type=0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825206649559711744 |
|---|---|
| author | Chen Tang Feng Xu Xiaoqun Zheng Guangxin Xiang |
| author_facet | Chen Tang Feng Xu Xiaoqun Zheng Guangxin Xiang |
| author_sort | Chen Tang |
| collection | DOAJ |
| description | Tuberculosis (TB) remains the leading cause of human mortality in infectious diseases. Drug-resistant TB, particularly multidrug-resistant TB and extensively drug-resistant TB, poses a pressing clinical and public health challenge. The main causative agents of TB are known as Mycobacterium tuberculosis (MTB), which exhibits a highly complex drug resistance profile. Traditional culture-based phenotypic drug susceptibility testing is time-consuming, and PCR-based assays are restricted to detecting known mutational hotspots. In this study, we present a protocol leveraging high-throughput nanopore sequencing technology in conjunction with multiplex PCR, termed targeted nanopore sequencing, for the identification of MTB and analysis of its drug resistance. Our method for MTB drug resistance assessment offers the benefits of being culture-free, efficient, high-throughput, and highly accurate, which could significantly aid in clinical patient management and the control of TB infections. |
| format | Article |
| id | doaj-art-dad81d1198f14d429feb7628fe5b7800 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-dad81d1198f14d429feb7628fe5b78002025-02-07T08:16:46ZengBio-protocol LLCBio-Protocol2331-83252025-02-0115310.21769/BioProtoc.5170Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing TechnologyChen Tang0Feng Xu1Xiaoqun Zheng2Guangxin Xiang3School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Laboratory Medicine, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaSchool of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Laboratory Medicine, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaSchool of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Laboratory Medicine, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaSchool of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Laboratory Medicine, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaTuberculosis (TB) remains the leading cause of human mortality in infectious diseases. Drug-resistant TB, particularly multidrug-resistant TB and extensively drug-resistant TB, poses a pressing clinical and public health challenge. The main causative agents of TB are known as Mycobacterium tuberculosis (MTB), which exhibits a highly complex drug resistance profile. Traditional culture-based phenotypic drug susceptibility testing is time-consuming, and PCR-based assays are restricted to detecting known mutational hotspots. In this study, we present a protocol leveraging high-throughput nanopore sequencing technology in conjunction with multiplex PCR, termed targeted nanopore sequencing, for the identification of MTB and analysis of its drug resistance. Our method for MTB drug resistance assessment offers the benefits of being culture-free, efficient, high-throughput, and highly accurate, which could significantly aid in clinical patient management and the control of TB infections.https://bio-protocol.org/en/bpdetail?id=5170&type=0 |
| spellingShingle | Chen Tang Feng Xu Xiaoqun Zheng Guangxin Xiang Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing Technology Bio-Protocol |
| title | Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing Technology |
| title_full | Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing Technology |
| title_fullStr | Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing Technology |
| title_full_unstemmed | Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing Technology |
| title_short | Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing Technology |
| title_sort | identification of mycobacterium tuberculosis and its drug resistance by targeted nanopore sequencing technology |
| url | https://bio-protocol.org/en/bpdetail?id=5170&type=0 |
| work_keys_str_mv | AT chentang identificationofmycobacteriumtuberculosisanditsdrugresistancebytargetednanoporesequencingtechnology AT fengxu identificationofmycobacteriumtuberculosisanditsdrugresistancebytargetednanoporesequencingtechnology AT xiaoqunzheng identificationofmycobacteriumtuberculosisanditsdrugresistancebytargetednanoporesequencingtechnology AT guangxinxiang identificationofmycobacteriumtuberculosisanditsdrugresistancebytargetednanoporesequencingtechnology |