Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s Disease
Objectives. This study compares glycoproteomes in Thai Alzheimer’s disease (AD) patients with those of cognitively normal individuals. Methods. Study participants included outpatients with clinically diagnosed AD (N=136) and healthy controls without cognitive impairment (N=183). Blood samples were c...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2021/1434076 |
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| author | Naphatthakarn Kerdsaeng Sittiruk Roytrakul Suwannee Chanprasertyothin Piangporn Charernwat Sirintorn Chansirikarnjana Piyamitr Sritara Jintana Sirivarasai |
| author_facet | Naphatthakarn Kerdsaeng Sittiruk Roytrakul Suwannee Chanprasertyothin Piangporn Charernwat Sirintorn Chansirikarnjana Piyamitr Sritara Jintana Sirivarasai |
| author_sort | Naphatthakarn Kerdsaeng |
| collection | DOAJ |
| description | Objectives. This study compares glycoproteomes in Thai Alzheimer’s disease (AD) patients with those of cognitively normal individuals. Methods. Study participants included outpatients with clinically diagnosed AD (N=136) and healthy controls without cognitive impairment (N=183). Blood samples were collected from all participants for biochemical analysis and for Apolipoprotein E (APOE) genotyping by real-time TaqMan PCR assays. Comparative serum glycoproteomic profiling by liquid chromatography-tandem mass spectrometry was then performed to identify differentially abundant proteins with functional relevance. Results. Statistical differences in age, educational level, and APOE ɛ3/ɛ4 and ɛ4/ɛ4 haplotype frequencies were found between the AD and control groups. The frequency of the APOE ɛ4 allele was significantly higher in the AD group than in the control group. In total, 871 glycoproteins were identified, including 266 and 259 unique proteins in control and AD groups, respectively. There were 49 and 297 upregulated and downregulated glycoproteins, respectively, in AD samples compared with the controls. Unique AD glycoproteins were associated with numerous pathways, including Alzheimer’s disease-presenilin pathway (16.6%), inflammation pathway mediated by chemokine and cytokine signaling (9.2%), Wnt signaling pathway (8.2%), and apoptosis signaling pathway (6.7%). Conclusion. Functions and pathways associated with protein-protein interactions were identified in AD. Significant changes in these proteins can indicate the molecular mechanisms involved in the pathogenesis of AD, and they have the potential to serve as AD biomarkers. Such findings could allow us to better understand AD pathology. |
| format | Article |
| id | doaj-art-dad5cd1f47e940beba8185bc11ad7a1e |
| institution | Kabale University |
| issn | 1875-8584 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-dad5cd1f47e940beba8185bc11ad7a1e2025-02-03T01:26:56ZengWileyBehavioural Neurology1875-85842021-01-01202110.1155/2021/1434076Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s DiseaseNaphatthakarn Kerdsaeng0Sittiruk Roytrakul1Suwannee Chanprasertyothin2Piangporn Charernwat3Sirintorn Chansirikarnjana4Piyamitr Sritara5Jintana Sirivarasai6Doctor of Philosophy Programme in Molecular MedicineFunctional Ingredients and Food Innovation Research GroupResearch & InnovationDepartment of MedicineDepartment of MedicineDepartment of MedicineGraduate Program in NutritionObjectives. This study compares glycoproteomes in Thai Alzheimer’s disease (AD) patients with those of cognitively normal individuals. Methods. Study participants included outpatients with clinically diagnosed AD (N=136) and healthy controls without cognitive impairment (N=183). Blood samples were collected from all participants for biochemical analysis and for Apolipoprotein E (APOE) genotyping by real-time TaqMan PCR assays. Comparative serum glycoproteomic profiling by liquid chromatography-tandem mass spectrometry was then performed to identify differentially abundant proteins with functional relevance. Results. Statistical differences in age, educational level, and APOE ɛ3/ɛ4 and ɛ4/ɛ4 haplotype frequencies were found between the AD and control groups. The frequency of the APOE ɛ4 allele was significantly higher in the AD group than in the control group. In total, 871 glycoproteins were identified, including 266 and 259 unique proteins in control and AD groups, respectively. There were 49 and 297 upregulated and downregulated glycoproteins, respectively, in AD samples compared with the controls. Unique AD glycoproteins were associated with numerous pathways, including Alzheimer’s disease-presenilin pathway (16.6%), inflammation pathway mediated by chemokine and cytokine signaling (9.2%), Wnt signaling pathway (8.2%), and apoptosis signaling pathway (6.7%). Conclusion. Functions and pathways associated with protein-protein interactions were identified in AD. Significant changes in these proteins can indicate the molecular mechanisms involved in the pathogenesis of AD, and they have the potential to serve as AD biomarkers. Such findings could allow us to better understand AD pathology.http://dx.doi.org/10.1155/2021/1434076 |
| spellingShingle | Naphatthakarn Kerdsaeng Sittiruk Roytrakul Suwannee Chanprasertyothin Piangporn Charernwat Sirintorn Chansirikarnjana Piyamitr Sritara Jintana Sirivarasai Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s Disease Behavioural Neurology |
| title | Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s Disease |
| title_full | Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s Disease |
| title_fullStr | Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s Disease |
| title_full_unstemmed | Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s Disease |
| title_short | Serum Glycoproteomics and Identification of Potential Mechanisms Underlying Alzheimer’s Disease |
| title_sort | serum glycoproteomics and identification of potential mechanisms underlying alzheimer s disease |
| url | http://dx.doi.org/10.1155/2021/1434076 |
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