Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environment
Flammulina filiformis is an important edible and medicinal mushroom widely cultivated in East Asia, with its quality and health strongly influenced by associated microbial communities. However, limited data exist on the bacterial communities associated with F. filiformis cultivation in Chinese farms...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1429213/full |
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| author | Zhenghui Liu Zhenghui Liu Zhenghui Liu Yunlong Cong Frederick Leo Sossah Frederick Leo Sossah Hongyan Sheng Yu Li |
| author_facet | Zhenghui Liu Zhenghui Liu Zhenghui Liu Yunlong Cong Frederick Leo Sossah Frederick Leo Sossah Hongyan Sheng Yu Li |
| author_sort | Zhenghui Liu |
| collection | DOAJ |
| description | Flammulina filiformis is an important edible and medicinal mushroom widely cultivated in East Asia, with its quality and health strongly influenced by associated microbial communities. However, limited data exist on the bacterial communities associated with F. filiformis cultivation in Chinese farms. This study investigated bacterial communities associated with F. filiformis and its production environment using high-throughput 16S rRNA gene amplicon sequencing and culture-dependent methods. A total of 42 samples were collected from farms in Jilin and Guizhou provinces, China, for microbial community profiling. The analysis revealed diverse bacterial phyla, including Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Cyanobacteria. Genera such as Pseudomonas, Lactobacillus, Acinetobacter, Flavobacterium, and Phyllobacterium were identified, with notable regional variations in the relative abundance of Pseudomonas and Lactobacillus. Pathogenic species, including Pseudomonas tolaasii, Ewingella americana, Stenotrophomonas maltophilia, Pseudomonas sp., Lelliottia amnigena, and Janthinobacterium lividum, were identified through phenotypic, biochemical, and molecular analyses. Pathogenicity tests confirmed the disease-causing potential of P. tolaasii, E. americana, and J. lividum in F. filiformis. These findings highlight regional differences in bacterial community composition and emphasize the need for tailored management practices. This study contributes to safe, high-quality mushroom cultivation and provides insights into improved cultivation practices, including Mushroom Good Agricultural Practices (MGAP). |
| format | Article |
| id | doaj-art-dacc9ca8a96d473dbea0cb1ce61c1279 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-dacc9ca8a96d473dbea0cb1ce61c12792025-08-20T02:49:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14292131429213Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environmentZhenghui Liu0Zhenghui Liu1Zhenghui Liu2Yunlong Cong3Frederick Leo Sossah4Frederick Leo Sossah5Hongyan Sheng6Yu Li7Engineering and Research Center for Southwest Bio-pharmaceutical Resources of National Education Ministry, Guizhou University, Guiyang, ChinaEngineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, ChinaInstitute of Edible Fungi, Guizhou University, Guiyang, ChinaResearch Institute of Science and Technology, Guizhou University, Guiyang, ChinaEngineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, ChinaCouncil for Scientific and Industrial Research (CSIR), Oil Palm Research Institute, Coconut Research Programme, Sekondi, GhanaDepartment of Plant Pathology, Washington State University, Pullman, WA, United StatesEngineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, ChinaFlammulina filiformis is an important edible and medicinal mushroom widely cultivated in East Asia, with its quality and health strongly influenced by associated microbial communities. However, limited data exist on the bacterial communities associated with F. filiformis cultivation in Chinese farms. This study investigated bacterial communities associated with F. filiformis and its production environment using high-throughput 16S rRNA gene amplicon sequencing and culture-dependent methods. A total of 42 samples were collected from farms in Jilin and Guizhou provinces, China, for microbial community profiling. The analysis revealed diverse bacterial phyla, including Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Cyanobacteria. Genera such as Pseudomonas, Lactobacillus, Acinetobacter, Flavobacterium, and Phyllobacterium were identified, with notable regional variations in the relative abundance of Pseudomonas and Lactobacillus. Pathogenic species, including Pseudomonas tolaasii, Ewingella americana, Stenotrophomonas maltophilia, Pseudomonas sp., Lelliottia amnigena, and Janthinobacterium lividum, were identified through phenotypic, biochemical, and molecular analyses. Pathogenicity tests confirmed the disease-causing potential of P. tolaasii, E. americana, and J. lividum in F. filiformis. These findings highlight regional differences in bacterial community composition and emphasize the need for tailored management practices. This study contributes to safe, high-quality mushroom cultivation and provides insights into improved cultivation practices, including Mushroom Good Agricultural Practices (MGAP).https://www.frontiersin.org/articles/10.3389/fmicb.2024.1429213/fullFlammulina filiformiscultivation environment16S ampliconbacterial diversitycultivable bacteriapathogenicity determination |
| spellingShingle | Zhenghui Liu Zhenghui Liu Zhenghui Liu Yunlong Cong Frederick Leo Sossah Frederick Leo Sossah Hongyan Sheng Yu Li Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environment Frontiers in Microbiology Flammulina filiformis cultivation environment 16S amplicon bacterial diversity cultivable bacteria pathogenicity determination |
| title | Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environment |
| title_full | Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environment |
| title_fullStr | Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environment |
| title_full_unstemmed | Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environment |
| title_short | Identification of bacterial communities associated with needle mushroom (Flammulina filiformis) and its production environment |
| title_sort | identification of bacterial communities associated with needle mushroom flammulina filiformis and its production environment |
| topic | Flammulina filiformis cultivation environment 16S amplicon bacterial diversity cultivable bacteria pathogenicity determination |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1429213/full |
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