EXPERIMENTAL EVALUATION OF THE METHODS FOR <i>FRANCISELLA TULARENSIS </i>15 NIIEG BIOMASS CONCENTRATION TO BE USED IN MANUFACTURING OF LIVE TULAREMIA VACCINE

The possibility of applying sedimentation, centrifuge, and filtration techniques for concentration of F. tularensis 15 NIIEG suspensions obtained after submerged cultivation has been evaluated. It is established that realization of tangential ultrafiltration as part of technological process of live...

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Bibliographic Details
Main Authors: D. N. Bibikov, O. A. Volokh, A. V. Komissarov, Yu. I. Samokhvalova, N. G. Avdeeva, A. K. Nikiforov
Format: Article
Language:Russian
Published: LLC Center of Pharmaceutical Analytics (LLC «CPHA») 2019-01-01
Series:Разработка и регистрация лекарственных средств
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Online Access:https://www.pharmjournal.ru/jour/article/view/418
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Summary:The possibility of applying sedimentation, centrifuge, and filtration techniques for concentration of F. tularensis 15 NIIEG suspensions obtained after submerged cultivation has been evaluated. It is established that realization of tangential ultrafiltration as part of technological process of live tularemia vaccine production is of distinct advantage. Characteristics of Francisella tularensis 15 NIIEG concentrate stored under different temperature/time-dependent conditions have also been studies. It is demonstrated that it is practical to preserve them at 4 °C within ≤6 days.
ISSN:2305-2066
2658-5049