Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner
The dietary habits of fruit flies profoundly influence their fitness, morphology, and physiology yet the mechanisms underlying these effects remain incompletely understood. To address this gap, the relationship between dietary regimens and the composition and function of adult <i>Drosophila me...
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MDPI AG
2025-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/16/2/141 |
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| author | Repac Jelena Trajković Jelena Rakić Marija Lunić Tanja Savić Tatjana Božić Bojan Božić Nedeljković Biljana Sofija Pavković-Lučić |
| author_facet | Repac Jelena Trajković Jelena Rakić Marija Lunić Tanja Savić Tatjana Božić Bojan Božić Nedeljković Biljana Sofija Pavković-Lučić |
| author_sort | Repac Jelena |
| collection | DOAJ |
| description | The dietary habits of fruit flies profoundly influence their fitness, morphology, and physiology yet the mechanisms underlying these effects remain incompletely understood. To address this gap, the relationship between dietary regimens and the composition and function of adult <i>Drosophila melanogaster</i> microbiota was investigated in the present study. The adult fly microbiota communities that were reared for long time on five different diets were characterized by means of 16S rRNA sequencing. Obtained results revealed distinct community structures associated with each dietary regimen, which was additionally corroborated through machine learning-based analysis. In general, sugar-rich diets correlate with microbial ecosystems of higher richness/diversity. Dominance of the phyla <i>Proteobacteria</i> and <i>Firmicutes</i> in the microbiota was confirmed irrespective of diet, with the varying proportions of the most abundant families: <i>Acetobacteraceae</i>, <i>Lactobacillaceae</i>, <i>Moraxellaceae</i>, <i>Bradyrhizobiaceae</i>, and <i>Leucostonocaceae</i>. Bacterial families of lower abundance also emerged as differentially present among the studied fly groups. Additionally, functional prediction provided initial clues into how nutrient availability might modulate the metabolic traits of adult fly microbiota in a sex-specific manner to meet host metabolic needs. Overall, the presented findings highlight the intricate interplay between diet, microbiota composition, and host phenotype in fruit flies, underscoring the importance of diet as a determinant of host-microbiota interactions. |
| format | Article |
| id | doaj-art-dac859ae4b4f4f3dbd4be287678be665 |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-dac859ae4b4f4f3dbd4be287678be6652025-08-20T02:45:00ZengMDPI AGInsects2075-44502025-02-0116214110.3390/insects16020141Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific MannerRepac Jelena0Trajković Jelena1Rakić Marija2Lunić Tanja3Savić Tatjana4Božić Bojan5Božić Nedeljković Biljana6Sofija Pavković-Lučić7Faculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković” National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaThe dietary habits of fruit flies profoundly influence their fitness, morphology, and physiology yet the mechanisms underlying these effects remain incompletely understood. To address this gap, the relationship between dietary regimens and the composition and function of adult <i>Drosophila melanogaster</i> microbiota was investigated in the present study. The adult fly microbiota communities that were reared for long time on five different diets were characterized by means of 16S rRNA sequencing. Obtained results revealed distinct community structures associated with each dietary regimen, which was additionally corroborated through machine learning-based analysis. In general, sugar-rich diets correlate with microbial ecosystems of higher richness/diversity. Dominance of the phyla <i>Proteobacteria</i> and <i>Firmicutes</i> in the microbiota was confirmed irrespective of diet, with the varying proportions of the most abundant families: <i>Acetobacteraceae</i>, <i>Lactobacillaceae</i>, <i>Moraxellaceae</i>, <i>Bradyrhizobiaceae</i>, and <i>Leucostonocaceae</i>. Bacterial families of lower abundance also emerged as differentially present among the studied fly groups. Additionally, functional prediction provided initial clues into how nutrient availability might modulate the metabolic traits of adult fly microbiota in a sex-specific manner to meet host metabolic needs. Overall, the presented findings highlight the intricate interplay between diet, microbiota composition, and host phenotype in fruit flies, underscoring the importance of diet as a determinant of host-microbiota interactions.https://www.mdpi.com/2075-4450/16/2/141<i>Drosophila melanogaster</i>adult fly microbiotametabolismnutritionsex |
| spellingShingle | Repac Jelena Trajković Jelena Rakić Marija Lunić Tanja Savić Tatjana Božić Bojan Božić Nedeljković Biljana Sofija Pavković-Lučić Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner Insects <i>Drosophila melanogaster</i> adult fly microbiota metabolism nutrition sex |
| title | Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner |
| title_full | Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner |
| title_fullStr | Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner |
| title_full_unstemmed | Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner |
| title_short | Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner |
| title_sort | different long term nutritional regimens of i drosophila melanogaster i shape its microbiota and associated metabolic activity in a sex specific manner |
| topic | <i>Drosophila melanogaster</i> adult fly microbiota metabolism nutrition sex |
| url | https://www.mdpi.com/2075-4450/16/2/141 |
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